Klhl3 (NM_001195075) Mouse Untagged Clone

CAT#: MC220053

Klhl3 (untagged) - Mouse kelch-like 3 (Drosophila) (Klhl3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhl3
• Synonyms: 7530408C15Rik; AI430941; EG627648
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220053 representing NM_001195075
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTACTATATCATGATCCCATGCCAGGTTGGAAAGCGTGGCCATAGGCTCTGGACTTGGACGAGAG
  TTGCAGCACGGATCAACGTGGCCTTATGCTTCGTAGGAGGAGAAAGTGCCTGGTTCCTGCTAGTGTCCAG
  GCTGACCTCCGCATCAGGGGGAAAGGGTTTGAGTGTCAAGCCGAGCCCTCAGCCCACGGCGCAGGCTGAG
  GATGAAGAGAAGAACCGGAGGACTGTAACCGTCAACGCTGCCCACATGGGGAAGGCCTTCAAGGTCATGA
  ATGAGCTGCGAAGTAAGCGGCTGCTGTGTGACGTGATGATCGTGGCTGAAGACGTGGAAGTAGAAGCTCA
  CCGTGTGGTCCTGGCAGCCTGCAGCCCTTACTTCTGTGCAATGTTCACAGGTGACATGTCTGAGAGCAAG
  GCCAAGAAGATCGAAATCAAGGACGTGGACGGACAGACTCTCAGCAAGCTCATCGACTACATCTACACCG
  CAGAGATCGAGGTGACAGAAGAGAACGTGCAGGTGCTGCTTCCAGCAGCCAGCTTGCTGCAGCTCATGGA
  TGTGCGACAGAACTGCTGTGATTTCCTGCAGTCTCAGCTGCATCCCACCAACTGCCTGGGCATCCGAGCT
  TTTGCAGATGTGCACACGTGCACCGACCTCCTGCAGCAGGCGAACGCCTATGCAGAGCAGCACTTCCCAG
  AGGTGATGCTGGGGGAAGAGTTTCTGAGCTTAAGTCTGGACCAGGTGTGCAGCTTGATATCCAGCGACAA
  GCTGACGGTCTCCTCAGAAGAGAAGGTGTTCGAAGCTGTGATTTCATGGATCAATTACGAGAAGGAGACT
  CGCCTAGACCACATGGCAAAGCTGATGGAGCACGTCCGGCTCCCTCTGTTACCGAGAGATTACCTGGTCC
  AGACAGTTGAGGAGGAAGCTTTGATCAAGAACAACAACACCTGTAAGGACTTCCTCATCGAAGCCATGAA
  GTACCACCTCCTGCCTCTTGACCAGAGGCTCTTGATCAAGAACCCCAGGACCAAGCCCCGGACTCCAGTC
  AGCCTGCCCAAGGTCATGATTGTGGTTGGTGGCCAGGCACCCAAGGCGATCCGCAGCGTGGAGTGCTATG
  ACTTCGAGGAGGGCCGCTGGGACCAGATAGCCGAGCTTCCCTCCAGAAGATGCAGAGCAGGTGTGGTGTT
  CATGGCAGGCCACGTGTATGCCGTGGGTGGCTTTAACGGCTCTCTGCGGGTCCGGACAGTGGATGTGTAC
  GATGGAGTGAAGGACCAGTGGACGTCCATTGCCAGCATGCAGGAGCGCCGGAGCACCCTGGGTGCGGCCG
  TGCTCAATGACCTTCTCTACGCAGTCGGAGGCTTCGACGGCAGTACCGGCCTGGCATCCGTAGAAGCCTA
  CAGCTACAAGACCAATGAGTGGTTCTTCGTGGCCCCTATGAACACACGGCGGAGCAGCGTGGGAGTGGGT
  GTTGTGGAGGGGAAGCTGTATGCCGTCGGGGGTTACGATGGTGCTTCCAGGCAGTGCCTGAGCACCGTGG
  AGCAGTACAACCCAGCCACCAACGAGTGGATATACGTGGCAGACATGAGCACCCGACGCAGTGGCGCTGG
  GGTTGGGGTGCTCAGCGGACAGCTGTATGCCACCGGGGGACATGACGGACCCTTGGTGAGGAAGAGTGTT
  GAAGTTTACGATCCTGGGACAAACACTTGGAAGCAGGTGGCAGACATGAACATGTGCCGGCGCAACGCAG
  GGGTCTGTGCGGTGAATGGGCTACTGTACGTAGTTGGAGGGGACGACGGATCGTGCAATCTGGCTTCAGT
  AGAGTACTACAACCCAGTCACAGACAAGTGGACACTGCTGCCCACCAACATGAGCACTGGGAGGAGCTAT
  GCAGGTGTTGCTGTGATTCACAAATCCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195075
• Insert Size: 1923 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195075.1, NP_001182004.1
• RefSeq Size: 2142 bp
• RefSeq ORF: 1923 bp
• Locus ID: 100503085
• UniProt ID: E0CZ16
• Cytogenetics: 13 B1
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron (PubMed:25831548, PubMed:28052936). The BCR(KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation (By similarity). The BCR(KLHL3) complex also mediates ubiquitination and degradation of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney (PubMed:25831548).[UniProtKB/Swiss-Prot Function]