Wdr26 (NM_145514) Mouse Untagged Clone

CAT#: MC220036

Wdr26 (untagged) - Mouse WD repeat domain 26 (Wdr26), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wdr26
• Synonyms: 1600024A01Rik; AA693241; AI447817; AU044014; C77982; Gid7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220036 representing NM_145514
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCCAACGGGGCGGGAGGAGGCGGCGGCGGGGGCGGCGGGGGCCAGGGCCAGACCCCGGAGCTCG
  CCTGCCTGTCGGCGCAGAACGGGGAGTCGTCCCCTTCGGCGACGTCCGCGGGGGACCTGGCCCACGCCAA
  CGGGCTCCTGCCTGCCGCCCCCTCCGCCGCTGGCAACAATAGCAACAGCCTGAGTGTCAATAACGGGGTT
  CCCGGCGGGGCCGCCGCGGCCTCCGCCACCGCCGCCGCCGCCCAGGCCACCCCCGAGCTGGGCAGCAGCC
  TCAAGAAGAAGAAGCGGCTCTCGCAGTCCGATGAGGATGTCATTAGGCTCATAGGACAGCACCTCAATGG
  CCTAGGGCTCAACCAGACTGTTGATCTCCTCATGCAAGAGTCAGGATGTCGTTTAGAGCATCCTTCTGCT
  ACCAAATTCCGAAATCATGTCATGGAAGGAGACTGGGATAAGGCAGAGAATGACCTGAATGAGCTAAAGC
  CTTTAGTGCATTCTCCTCACGCTATTGTGGTAAGAGGCGCACTTGAAATCTCTCAAACGTTGTTGGGAAT
  AATTGTGAGGATGAAGTTTCTGCTGCTGCAGCAGAAGTACCTGGAATACCTGGAGGACGGCAAGGTCCTG
  GAGGCACTTCAAGTTCTACGCTGCGAACTGACGCCGTTGAAATACAACACCGAGCGCATCCATGTCCTTA
  GTGGGTATCTGATGTGCAGCCATGCCGAAGACCTACGGGCAAAAGCTGAATGGGAAGGCAAGGGCACAGC
  GTCCCGGTCCAAACTGCTGGACAAGCTTCAGACCTATTTACCACCATCAGTGATGCTTCCCCCACGGCGT
  TTACAGACTCTCCTGCGGCAGGCGGTGGAACTACAAAGGGATCGGTGCCTATATCACAATACCAAACTTG
  ACAATAATCTAGATTCTGTGTCTCTACTTATAGATCATGTTTGTAGTAGGAGGCAGTTTCCCTGTTACAC
  TCAACAGATACTTACAGAGCATTGTAATGAAGTGTGGTTCTGTAAATTTTCTAATGATGGCACTAAACTA
  GCAACAGGATCAAAGGATACCACAGTTATCATATGGCAAGTTGATCCGGATACGCACCTGTTAAAACTGC
  TTAAAACGTTAGAAGGACATGCGTATGGTGTCTCTTATATAGCATGGAGTCCAGATGACAGCTATCTTGT
  TGCTTGTGGTCCAGATGACTGCTCTGAGCTTTGGCTTTGGAATGTACAGACGGGAGAATTAAGAACAAAA
  ATGAGCCAATCTCATGAAGACAGTTTGACCAGTGTGGCCTGGAATCCAGATGGGAAACGCTTTGTGACGG
  GAGGTCAGCGTGGTCAGTTCTACCAGTGTGACCTGGACGGAAATCTTCTGGACTCCTGGGAAGGAGTGCG
  AGTACAGTGCCTGTGGTGCTTGAGTGACGGGAAGACTGTGCTGGCTTCCGACACGCACCAGAGAGTCCGG
  GGCTACAACTTTGAGGACCTGACAGATAGAAACATAGTACAGGAAGATCATCCTATTATGTCATTTACTA
  TTTCCAAAAATGGCCGATTAGCTTTGTTAAATGTAGCAACTCAGGGAGTTCATTTATGGGACTTGCAAGA
  CAGAGTTTTAGTAAGGAAATATCAAGGTGTTACCCAAGGGTTTTATACAATCCACTCGTGTTTTGGAGGC
  CATAATGAAGACTTCATTGCTAGTGGCAGCGAAGATCACAAGGTTTACATCTGGCACAAACGTAGTGAAC
  TGCCAATTGCGGAGCTCACAGGGCACACGCGCACAGTAAATTGTGTTAGCTGGAACCCACAGATTCCATC
  CATGATGGCCAGTGCCTCAGACGATGGCACTGTTAGAATATGGGGACCAGCACCTTTCATAGACCACCAG
  AATATTGAAGAGGAATGCAGTAGCATGGATAGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145514
• Insert Size: 1926 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145514.5, NP_663489.4
• RefSeq Size: 7063 bp
• RefSeq ORF: 1926 bp
• Locus ID: 226757
• UniProt ID: Q8C6G8
• Cytogenetics: 1 H4
• Gene Summary: G-beta-like protein involved in cell signal transduction. Acts as a negative regulator in MAPK signaling pathway. Functions as a scaffolding protein to promote G beta:gamma-mediated PLCB2 plasma membrane translocation and subsequent activation in leukocytes. Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (By similarity). Acts as a negative regulator of the canonical Wnt signaling pathway through preventing ubiquitination of beta-catenin CTNNB1 by the beta-catenin destruction complex, thus negatively regulating CTNNB1 degradation. Serves as a scaffold to coordinate PI3K/AKT pathway-driven cell growth and migration. Protects cells from oxidative stress-induced apoptosis via the down-regulation of AP-1 transcriptional activity as well as by inhibiting cytochrome c release from mitochondria (By similarity). Protects also cells by promoting hypoxia-mediated autophagy and mitophagy (By similarity).[UniProtKB/Swiss-Prot Function]