Zfp143 (NM_009281) Mouse Untagged Clone

CAT#: MC219983

Zfp143 (untagged) - Mouse zinc finger protein 143 (Zfp143), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp143
• Synonyms: AA959806; AU015869; D7Ertd805e; KRAB14; pHZ-1; SBF; Staf; Zfp79; Zfp80-rs1; Znf143
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219983 representing NM_009281
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTTAGCACAAATAAATCGGGATTCTCAGGGAATGACAGAATTTCCTGGAGGAGGAATGGAGGCTC
  AACATGTTACCCTGTGCCTGACGGAGGCAGTAACCGTGGCAGATGGTGACAATTTAGAAAATATGGAAGG
  TGTGAGTTTACAAGCTGTGACACTCGCAGACGGCTCTACTGCTTACATACAGCATAATTCTAAAGACGGG
  AGACTCATTGATGGCCAGGTCATTCAGCTGGAAGATGGTTCTGCTGCCTATGTTCAGCATGTCCCCATAC
  CTAAAAGTAGGGACAGTTTGCGGCTGGAGGATGGGCAAGCAGTGCAGCTAGAAGACGGGACCACTGCATT
  TATCCACCACACCTCGAAAGACAGTTATGACCAGAGCTCACTTCAGGCCGTTCAGTTAGAAGATGGGACA
  ACAGCTTATATCCACCATGCAGTGCAAGTCCCACAGTCTGATACCATCTTGGCAATTCAGGCTGATGGGA
  CAGTGGCAGGGCTGCACACTGGGGATGCCACAATTGACCCAGATACCATTAGTGCTTTGGAGCAGTACGC
  AGCAAAGGTATCCATTGATGGCAGTGACGGTGTAACAAGTACAGGAATGATTGGAGAGAATGAGCAAGAG
  AAAAAAATGCAGATTGTCTTACAAGGCCATGCTACTCGGGTAACTCCTAAATCTCAGCAGAGTGGAGAAA
  AGGCATTTCGGTGCAAATACGACGGCTGTGGGAAACTCTATACAACAGCTCATCACCTTAAGGTCCATGA
  GCGCTCACACACAGGAGACCGGCCTTACCAGTGTGAGCATTCAGGCTGTGGGAAAGCATTTGCAACAGGT
  TATGGATTAAAAAGTCATTTCAGAACTCATACTGGAGAAAAGCCATATCGGTGTTCAGAAGATAATTGTA
  CAAAGTCTTTCAAAACTTCAGGAGATCTACAGAAGCACATCAGAACCCATACAGGAGAAAGGCCCTTTAA
  GTGTCCTATTGAAGGCTGTGGTCGGTCCTTTACCACATCAAATATCAGAAAAGTGCACATTAGGACACAC
  ACAGGAGAAAGACCTTATTACTGCACAGAACCTGGATGTGGGAGGGCATTTGCCAGCGCAACTAATTATA
  AAAACCATGTGAGGATACACACAGGAGAAAAGCCATATGTCTGTACAGTTCCTGGGTGTGACAAACGGTT
  TACAGAATATTCCAGTTTGTATAAACACCATGTCGTTCACACTCACTCTAAACCATACAACTGTAACCAC
  TGTGGGAAGACGTACAAACAGATTTCCACACTGGCCATGCACAAACGGACAGCCCACAACGACACAGAGC
  CCATTGAGGAGGAGCAGGAAGCCTTCTTCGAGCCTCCCCCAGGTCAAGGTGATGATGTTCTTAAAGGGTC
  CCAGATCACATATGTTACAGGTGTAGATGGAGAGGACATTGTATCTACACAAGTAGCCACAGTAACCCAG
  TCTGGGCTGAGTCAACAAGTCACACTCATATCACAGGATGGGACTCAACATGTCAACATATCTCAAGCTG
  ACATGCAGGCCATTGGCAACACCATCACCATGGTAACACAGGATGGCACACCCATCACGGTCCCCACTCA
  CGATGCAGTCATCTCCTCAGCAGGAACACATTCTGTTGCTATGGTTACCGCGGAGGGCACAGAAGGACAG
  CAGGTCGCAATTGTAGCTCAAGATCTGGCAGCGTTCCATACAGCATCTTCAGAGATGGGACACCAACAAC
  ATAGCCATCACCTAGTAACTACAGAAACCAGACCTCTGACCTTAGTGGCAACATCCAACGGCACCCAGAT
  CGCAGTTCAGCTTGGAGAGCAGCCGTCTCTGGAAGAAGCTATCAGAATAGCATCGAGGATCCAACAAGGG
  GAGACGCCAGGGTTGGATGATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009281
• Insert Size: 1914 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009281.3, NP_033307.2
• RefSeq Size: 3013 bp
• RefSeq ORF: 1914 bp
• Locus ID: 20841
• UniProt ID: O70230
• Cytogenetics: 7 57.7 cM
• Gene Summary: Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 (By similarity).[UniProtKB/Swiss-Prot Function]