Scnn1b (NM_011325) Mouse Untagged Clone

CAT#: MC219982

Scnn1b (untagged) - Mouse sodium channel, nonvoltage-gated 1 beta (Scnn1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Scnn1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219982 representing NM_011325
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGTGAAGAAGTACCTCCTGAAGTGCCTGCACCGGCTGCAGAAGGGCCCAGGCTACACCTACAAGG
  AGCTGCTAGTGTGGTACTGCAATAACACCAACACCCACGGCCCCAAACGCATCATCTGTGAGGGGCCCAA
  GAAGAAGGCCATGTGGTTCCTGCTTACGCTGCTCTTCGCCTGCCTGGTGTGCTGGCAGTGGGGAGTCTTC
  ATCCAGACCTACCTGAGCTGGGAGGTCAGCGTCTCGCTCTCCATGGGCTTCAAGACGATGAACTTCCCGG
  CGGTCACCGTCTGCAATTCCAGCCCCTTCCAGTACTCCAAGGTCAAGCACTTGCTCAAGGACTTGGATGA
  GCTAATGGAGGCAGTCCTGGAAAAGATTCTGGCTCCAGAGGCCAGCCACAGCAACACCACCAGGACCCTG
  AACTTTACCATCTGGAACCACACACCCCTGGTCCTTATTGATGAGCGGAACCCTGACCACCCGGTGGTTC
  TCAATTTGTTTGGGGACAGCCACAACAGCAGCAACCCGGCTCCAGGAAGCACCTGCAATGCCCAGGGATG
  CAAAGTGGCCATGAGGCTGTGCAGTGCCAATGGAACTGTGTGTACCTTGCGGAACTTCACCAGTGCCACC
  CAGGCCGTAACTGAGTGGTACATCCTGCAGGCCACCAACATCTTCTCACAAGTGCTTCCTCAGGACCTGG
  TGGGGATGGGCTATGCCCCTGATCGCATAATCCTAGCTTGCCTGTTTGGAACAGAGCCCTGCAGTCATCG
  GAACTTCACGCCTATCTTCTACCCTGATTATGGCAACTGCTACATCTTCAACTGGGGCATGACAGAGGAG
  ACACTTCCTTCTGCCAACCCTGGGACTGAATTTGGTCTCAAGTTGATCCTGGACATCGGTCAGGAGGACT
  ATGTCCCCTTCCTTGCGTCCACAGCAGGGGCTAGGCTGATGCTTCATGAGCAGAGGACGTACCCCTTCAT
  TAGAGAGGAGGGCATCTATGCCATGGCAGGAACTGAGACGTCTATTGGGGTGCTGGTGGACAAGCTGCAA
  CGCAAGGGGGAGCCCTACAGTCCCTGCACCATGAACGGCTCCGATGTTGCCATAAAGAACCTCTACAGTG
  TCTACAACACCACCTATTCCATCCAGGCCTGTCTTCATTCCTGTTTCCAAGACCACATGATCCGTAACTG
  CAGTTGTGGCCACTACTTATACCCACTGCCTGAAGGAGAGAAATACTGCAACAACAGGGACTTCCCAGAC
  TGGGCCTATTGCTATCTAAACCTGCAGATGAGTGTGACCCAGAGAGAGACCTGCCTCAGCATGTGCAAGG
  AGTCCTGCAATGACACCCAGTATAAGATGACCATCTCCATGGCTGACTGGCCATCTGAGGCCTCTGAGGA
  TTGGATCTTACATGTCCTGTCTCAGGAGCGGGACCAGAGCTCGAATATCACCCTGAGCAGGAAGGGTATT
  GTCAAGCTCAATATCTACTTCCAAGAGTTCAACTACCGTACCATTGAGGAATCTCCAGCCAACAATATTG
  TGTGGCTGCTCTCTAACCTGGGGGGCCAGTTTGGCTTCTGGATGGGGGGCTCGGTGCTGTGCCTCATTGA
  GTTTGGGGAGATCATTATCGACTTCATTTGGATCACCATCATCAAGCTGGTGGCCTCCTGCAAAGGCCTG
  CGCAGGAGGCGGCCACAGGCACCCTACACTGGCCCACCGCCCACTGTGGCTGAGCTGGTAGAGGCCCACA
  CCAACTTTGGCTTCCAGCCTGACACAACCAGCTGCAGGCCCCACGGCGAGGTCTACCCTGACCAACAGAC
  TCTGCCCATCCCGGGGACTCCACCCCCCAACTATGACTCCCTGAGGCTGCAGCCGCTGGACACCATGGAG
  TCGGACAGTGAGGTGGAGGCCATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011325
• Insert Size: 1917 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011325.2, NP_035455.1
• RefSeq Size: 2694 bp
• RefSeq ORF: 1917 bp
• Locus ID: 20277
• UniProt ID: Q9WU38
• Cytogenetics: 7 65.07 cM
• Gene Summary: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shortest transcript. Both variants 1 and 2 encode the same protein.