Ctcfl (NM_001081387) Mouse Untagged Clone

CAT#: MC219960

Ctcfl (untagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctcfl
• Synonyms: Boris; OTTMUSG00000016680
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219960 representing NM_001081387
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCGCTGAGGTCCCTGTCCCTTCTGGGTACTTCACCCAGATCAAAGAGCAGAAGTTGAAGCCTG
  GAGACCTAGAGGAGGAGAAAGAGGAGGACGGGGTACAAAGAGTGGAAGCCCAGGAGGGAGTTGTCAAGGA
  GGTGGAGGCCGAGAACAGTTGCCTGCTTCTGGAGGCCAGGGCCCCGGTGGAGAGCGACAGGCGGATCCTG
  ACCCTGCAAACGGTGCACCTGGAGTCCCAGGATGTGCACCTACAGGGGCTGGGATGGCTGAGCGTGCCAC
  ACTCTGAGGAGCTTTCAGGGACGGTACCAGAGGCGGAAGGCATACTGCAGTTGCCATCCGTGCTGTGGCT
  CGACCCAGAGCCCCAGCTCAGCCTTCAGCATTGCGTGACGGTCAGCATCCCGGAAGAGCTGTACCCACCA
  GAGGAGCTGCAGCGGATACATTTTCACCTGCTGAGAGAGAATGTGCTAATGGCCGAGGAGAACCCAGAGT
  TAACACCAGACTTGGACGAAAGCACAGCCCTGAAAAAGCCCGAAGAAGATGAAAAGGACCAGCTCCCGCC
  CCAGGGAGAGACAGACAAGAGAGAAGAGAGGTTGCTCCTTCTGGAAATGAAACCAAAAGAGGGAAAAGAC
  GACGAAATTGTCCTGACCATTTCCCATCTAAGCCTCGAAGAACAGCAAGATCCACCAGCGGCCAATCAGA
  CAAGTGTGCCGGGAGCCAAAGCCGCAAAACCAAAACGGCGGAGGCAGACCAAGGGAAAGCCTCAGAGCTT
  TCAGTGTGACACCTGCCCGTTCACTTCCTCCAAGCTCTCAACTTTCAATCGTCACATCAAAATTCACAGC
  AATGAGAGGCCACACCTGTGTCACCTGTGCCTGAAGGCCTTCCGGACTGTCACTCTTCTTAGGAACCATG
  TGAACACCCACACAGGAACCAGGCCCCACAAGTGCAGGGACTGCGACATGGCGTTTGTCACCAGTGGAGA
  ACTCGTCCGGCACAGGCGTTACAAACACACTTATGAGAAGCCCTTCAAGTGCTCCCTGTGCAAGTACGCC
  AGCGTCGAGGCAAGCAAGATGAAGCGTCACATCCGCTCACACACGGGTGAGCGTCCCTTCCAGTGTTGCC
  AGTGTGCTTACGCCAGCAGGGACTCCTACAAGCTGAAGCGCCACATGAGGACACACTCAGGTGAGAAGCC
  GTATGAATGTCCCACCTGTCACGTCCGGTTCACCCAGAGCGGGACCATGAAAATCCATATAGCACAGAAG
  CACGGAGAGAATGTGCCCAAATACGAGTGTCCCCACTGTGCCACCATCATCGCGAGGAAGAGCGACCTGC
  GTGTCCATCTGCGTAACCTGCACAGCCAGAGCCCGGAGGAGATGAAGTGCCGATACTGTCCCGCTGGCTT
  CCATGAGCGCTATGCCCTCATTCAGCACCAGAGGACCCACAAGAACGAGAAGAAGTTCAAGTGCAAGCAG
  TGCGATTACGCGTGCAAGCAGGAGCGATGCTTGAAGGCGCACATGCGCATGCACACAGGAGAGAAGCCCT
  TCTCCTGCCTGGCCTGCAACAAGCACTTCCGACAGAAGCAGCTACTGACCGTGCACCTGAGGAAGTACCA
  TGACCCGAACTTCGTCCCCAATCTGCACCTGTGCCTCAAGTGTGATAAACGTTTCTCCCGCTGGAGTAAC
  CTGCAGAGACACAGAAAGAAGTGTGACCCGGAGCATGAGACGTTAGCCCCCAACAAGGACAGGAGACCAG
  TGACAAGGACACAGGCCTCGGAGGGAGAAGCAGGACACAAGGAAGGGGAGCCTCAGTGCCCTGGGGAGCA
  GGCTCTGGGCCACCAAGGAGAAGCAGCGGGGAGCCAGAGCCCAGACCACGGCCTTACCTGCGAGATAATC
  TTTAACATGATGGATAAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001081387
• Insert Size: 1911 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081387.2, NP_001074856.1
• RefSeq Size: 3217 bp
• RefSeq ORF: 1911 bp
• Locus ID: 664799
• UniProt ID: A2APF3
• Cytogenetics: 2 H3
• Gene Summary: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame 3' splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.