Crtc1 (NM_001004062) Mouse Untagged Clone

CAT#: MC219955

Crtc1 (untagged) - Mouse CREB regulated transcription coactivator 1 (Crtc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Crtc1
• Synonyms: AI413414; Mect1; mKIAA0616; R74955; TORC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219955 representing NM_001004062
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTTCGAACAATCCGCGGAAATTTAGCGAGAAGATCGCACTGCACAACCAGAAGCAGGCGGAGG
  AGACGGCGGCCTTCGAGGAGGTCATGAAGGACCTGAGCCTGACGCGGGCCGCGCGGCTTCAGCTGCAGAA
  GTCCCAGTACCTGCAGCTGGGCCCCAGCCGTGGCCAGTACTACGGTGGGTCCCTGCCCAACGTGAACCAG
  ATTGGAAGCAGCAGCGTGGACCTGGCCTTCCAGACCCCATTTCAGTCCTCAGGCCTGGACACGAGTCGGA
  CCACACGACATCATGGGCTTGTGGACAGAGTATATCGTGAGCGTGGCAGACTTGGCTCCCCGCACCGTCG
  ACCCCTGTCAGTAGACAAGCATGGGCGACAGGCTGACAGCTGCCCCTATGGCACCGTGTACCTCTCGCCT
  CCTGCGGACACCAGCTGGAGGAGGACCAACTCTGACTCTGCCCTGCACCAGAGCACAATGACACCCAGCC
  AGGCAGAGTCCTTCACAGGCGGGTCCCAGGATGCGCACCAGAAGAGAGTCTTACTGCTAACTGTCCCAGG
  AATGGAGGACACCGGGGCTGAGACAGACAAGACCCTTTCTAAGCAGTCATGGGACTCAAAGAAGGCGGGT
  TCCAGGCCCAAGTCCTGTGAGGTCCCCGGAATCAACATCTTTCCGTCTGCAGACCAGGAGAACACAACAG
  CCCTGATCCCTGCCACCCACAACACAGGGGGCTCCCTTCCTGACCTCACCAACATCCACTTCCCCTCCCC
  ACTCCCGACACCACTGGACCCTGAGGAGCCTCCGTTCCCTGCTCTCACCAGCTCCAGCAGCACCGGCAGC
  CTTGCACATCTGGGCGTTGGCGGCGCAGGCCAGGGTATGAACACCCCCAGCTCTTCTCCACAGCACCGGC
  CAGCAGTCGTCAGCCCCCTGTCCCTGAGCACAGAGGCCAGGCGGCAGCAGGCCCAGCAGGTGTCACCCAC
  CCTGTCTCCGTTGTCACCCATCACTCAGGCCGTGGCTATGGATGCCCTGTCCTTGGAGCAGCAGCTGCCC
  TATGCCTTCTTCACCCAGACTGGCTCCCAGCAGCCTCCCCCACAGCCCCAGCCACCGCCTCCACCTCCAC
  CGGTATCCCAGCAGCAGCCACCACCTCCACAGGTGTCTGTGGGCCTCCCCCAGGGTGGTCCACTGCTGCC
  CAGTGCCAGCCTGACTCGGGGGCCCCAGCTGCCACCACTCTCAGTTACTGTACCATCCACTCTTCCCCAG
  TCCCCTACAGAGAACCCAGGCCAGTCACCAATGGGGATCGATGCCACTTCGGCACCAGCTCTGCAGTACC
  GCACGAGTGCAGGGTCACCTGCCACCCAGTCTCCCACCTCTCCGGTCTCCAACCAAGGCTTCTCCCCTGG
  AAGCTCCCCACAGCACACGTCCACCCTGGGCAGCGTGTTTGGGGATGCGTACTATGAGCAGCAGATGACA
  GCCAGGCAGGCCAATGCTCTGTCCCGCCAGCTGGAGCAGTTCAACATGATGGAGAACGCCATCAGCTCCA
  GCAGCCTATACAACCCGGGCTCCACACTCAACTATTCCCAGGCTGCCATGATGGGTCTGAGCGGGAGCCA
  CGGGGGCCTACAGGACCCGCAGCAGCTCGGCTACACAGGCCACGGTGGAATCCCCAACATCATCCTCACG
  GTGACAGGAGAGTCACCACCGAGCCTCTCTAAGGAACTGAGCAGCACACTGGCAGGAGTCAGTGATGTCA
  GCTTTGATTCAGACCATCAGTTTCCACTGGACGAGCTGAAGATTGACCCTCTGACCCTGGACGGACTCCA
  TATGTTGAATGACCCAGACATGGTTTTAGCCGACCCAGCCACCGAGGACACCTTCCGAATGGACCGCCTG
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004062
• Insert Size: 1893 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004062.2, NP_001004062.1
• RefSeq Size: 5674 bp
• RefSeq ORF: 1893 bp
• Locus ID: 382056
• UniProt ID: Q68ED7
• Cytogenetics: 8 B3.3
• Gene Summary: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites (PubMed:29211348). Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated (PubMed:29211348). Acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells (By similarity). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons. In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.[UniProtKB/Swiss-Prot Function]