Trim25 (NM_009546) Mouse Untagged Clone

CAT#: MC219941

Trim25 (untagged) - Mouse tripartite motif-containing 25 (Trim25), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim25
• Synonyms: AA960166; AL022677; EFP; Zfp147
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219941 representing NM_009546
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGAATCCTCTGGCCGAGGAGCTGTCCTGCTCCGTCTGCCTGGAGCTCTTCAAGGAACCTG
  TCACCACTCCGTGCGGCCACAATTTCTGCATGTCATGCCTGGATGAGACGTGGGTCGTCCAGGGCCCGCC
  GTACCGATGCCCGCAATGTCGCAAAGTGTACCAGGTGCGCCCGCAGCTGCAAAAGAACACGGTAATGTGC
  GCGGTGGTGGAGCAGTTCCTGCAGGCCGAGCAGGCGCGGACCCCGGTGGACGACTGGACGCCTCCTGCCC
  GCTTCTCCGCCTCCAGCGCAGCCACCCAGGTGGCCTGCGACCACTGCCTGACGGAGATCGCAGTGAAGAC
  GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGAGGCCTCACTTCGACAGTCCGGCCTTCCAG
  GATCACCCCCTACAGTCGCCCATTCGTGACCTTCTGCGCCGCAAATGTACCCAGCACAACCGCCTTCGGG
  AATTGTTTTGTCCCGAGCATGGCGAGTGCATCTGCCATATCTGCTTGGTAGAGCACAAGACCTGCTCCCC
  TACGACCCTAAGTCAAGCCAGCGCCGACCTGGAGTACAAACTGAGGAATAAACTCACTATCATGCACAGC
  CACATCAATGGGGCAACCAAAGCATTGGAGGATGTGAGATCCAAGCAACAGTGTGTGCAGGATTCTATGA
  AGAGGAAGATGGAACAGCTGAGACAGGAGTATATGGAAATGAAGGCTGTCATCGACGCAGCAGAGACCAG
  CTCCTTGCGCAAGTTAAAGGAGGAGGAGAAGAGAGTCTACGGAAAATTCGACACCATCTACCAGGTTCTC
  GTCAAGAAGAAGAGTGAGATGCAGAAACTGAAGGCAGAGGTTGAGCTCATTATGGACAAAGGGGATGAGT
  TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAAGGAGAGTCAACAAAACCAGTCTACATCCCTAAGATAGA
  TCTGGATCATGATTTGATAATGGGAATCTACCAAGGGGCGGCGGACCTCAAGAGTGAACTGAAGCACTCC
  ATTAAGAAGCTACAGAAGAAGTCTGAGGAGCACAATGGCTCAGGTAACAAGGGAGACCAGACACAGTCCA
  CTTTTAAACCCGTACAACCTTCGAAGAAGACCATTCAAGAAAAGAAGACCAAGAAAACCCCTGTTGCTCC
  CGGCCCTCCCTCCCACTTCTCACCTAACAAGTTGCCCACCTTTGGAGCTCCTGGCCAATCACTGGATTCA
  AAAGCAACTTCCCCTGATGCCGCACCCAAAGCCAGTGCTGCACAGCCAGACTCTGTAGGAGTCAAGGCTA
  AGGTGCTAGAAAACTTCTTAACCAAGTCCAGAACGGAGCTTCTGGAGTATTTCGTGAAAGTCATCTTCGA
  CTACAATACCGCCCACAACAAAGTGTCTCTGTCAAACAAGTACACCACCGCCTCTGTGTCCGATGGGCTC
  CAGCACTACCGGTCCCATCCCCAGAGGTTCACCTACTGCTCTCAGGTCCTGGGGCTGCACTGCTACAAGA
  ACGGCATTCACTACTGGGAGGTGGAACTACAGAAGAACAACTTCTGCGGCGTAGGCATCTGCTACGGCAG
  CATGGAACGGCAGGGCCCCGAGAGCCGGCTGGGCCGCAACCCCAACTCATGGTGTGTGGAGTGGTTCAAT
  AACAAGATCTCTGCCTGGCACAACAACGTGGAGAAGACTCTGCCCTCCACCAAGGCCACTCGAGTCGGCG
  TGCTGCTCAACTGTGACCACGGCTTCGTCATTTTCTTTGCGGTCACCGAAAAGGTGCACCTGATGTACAA
  GTTCAAGGTGGACTTCACCGAAGCTCTGTACCCAGCCTTCTGGGTGTTCTCGGCCGGTACCACCCTCTCT
  ATCTGCTCCAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009546
• Insert Size: 1905 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009546.2, NP_033572.2
• RefSeq Size: 5596 bp
• RefSeq ORF: 1905 bp
• Locus ID: 217069
• UniProt ID: Q61510
• Cytogenetics: 11 C
• Gene Summary: Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.[UniProtKB/Swiss-Prot Function]