Dach2 (NM_033605) Mouse Untagged Clone

CAT#: MC219930

Dach2 (untagged) - Mouse dachshund 2 (Drosophila) (Dach2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dach2
• Synonyms: 9430028N04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219930 representing NM_033605
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTCTCTGCACCTCCGGTGATCTCTGCAACTTCCAGCAGCGCCGGCGTCCCGGGGGGTTTATTCC
  GGGCTGAACCCCTGTATTCGTCTCCAGGAGAGCCCCCTCGCCTAACCCCTAACATGATCAACAGTTTCAT
  GGCCAATAACCATAACGGCAGTGTCTTGGGTGGCGGGATTGGTGGTGGCAGCGGTGGCAGCAGCAACACC
  AACACAAACGAGTGCCGGATGGTCGACATGCACGGGGTGAAGGTAGCTTCATTCCTGATGGATGGCCAGG
  AACTGATCTGCCTGCCGCAAGTCTTTGACCTTTTCCTCAAGCACCTGGTGGGAGGGTTGCACACAGTGTA
  CACTAAGCTGAAGAGACTCGACATATCTCCGGTGGTGTGCACTGTAGAGCAAGTTCGAATCCTCCGTGGG
  CTGGGGGCCATCCAGCCAGGGGTGAATCGTTGCAAGCTCATCACCAGGAAAGACTTTGAAACTTTGTTCA
  CCGATTGCACCAACGCCAGAAGGAAGAGGCAAATGACAAGGAAACAAGCTGTTAACAGTTCAAGGCCTGG
  CAGGCCCCCTAAGCGTTCTTTGGGAGTATTGCAAGATAATGCCCGACTTCTGCCCCATGCAGTCCCAGGC
  CTCTTATCACCAGGACTGATCACTCCAACAGGTATCACAGCTGCAGCGATGGCTGAGGCCATGAAGCTCC
  AGAAGATGAAGCTTATGGCAATGAATACTCTTCAGGGTAATGGAAGCCAAAATGGGACTGAGTCAGAGCC
  AGATGACCTTAATTCTACGACAGGTGGAAGTGAATCCTCCTGGGATAAAGATAAGATACAATCTCCACTT
  GCTGCTTCTGGACCTCAACATGGAATTGCTCATGCAGCACTTGCTGGTCAGCCAGGCCTTGGAGGTGCTC
  CGACCCTTAATCCACTTCAGCAGAATCACCTGCTAAGCAATCGTCTGGATCTCCCATTTATGATGATGCC
  TCATCCCCTACTTCCAGTCAGCTTACCTCCTGCATCAGTTGCCATGGCAATGAATCAGATGAATCATCTC
  AATACTATTGCCAACATGGCTGCTGCAGCCCAGATTCACAGTCCACTCTCCAGAGCTGGTGCCTCTGTTA
  TAAAGGAACGGATCCCAGAGAGTCCTTCTCCTGCTCCCTCTCTGGAAGAGAGTCATCGTCCTGGGAGCCA
  GACCTCCTCCCACCCAAGCAGCAGTGTGTCCAGCTCTCCCTCGCAGATGGATCATCATTCAGAGAGAATG
  GTTATGATGCCCAACAATCGAGAAGAGCTTATTGTTGACCAAGATAATGGACAAAGCATAAAAAAATTCC
  AGAGGGATAATAAAGAAGAAGTACCAGCTCAAATCCCAGTCATGAAGTCACCCTTGGATAAGATCCAGTT
  GGCTCCTGGACAGGCATTGCATCCTGGATTCCCTGGACCATTCATTTTTGCAGATAGTCTATCTTCTGTG
  GAGACTCTGTTGACCAACATTCAGGGTCTACTGAAAGTGGCTTTGGATAATGCTCGTATCCAGGAGAAGC
  AGATTCAGCAGGAAAAGAAGGAACTGCGAATAGAGCTCTTCAGAGAAAGAGAAATTAGAGAAAACCTGGA
  GCGACAACTTGCAGTTGAGCTTCAAAGCAGAAGTACAATGCAAAAGCGTCTGAAAAAAGAGAAAAAGGCT
  AAGAGAAAACTTCAGGAAGCCTTGGAATTTGAATCAAAGCGCCGCGAGCAAGTGGAGCAAGCACTTAAAC
  AAGCTACATCTGGTGACAGTGGACTGAGAATGTTAAAAGATTCTGGAATTCCAGATATTGAAATAGAAAA
  CAGTGGAACGCCGCATGACAGTGCTGCTATGCAAGGAGGTAACTATTACTGTTTAGCAATGGCACAGCAG
  TTGTGTTCAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033605
• Insert Size: 1905 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033605.2, NP_291083.1
• RefSeq Size: 3014 bp
• RefSeq ORF: 1905 bp
• Locus ID: 93837
• UniProt ID: Q925Q8
• Cytogenetics: X 49.13 cM
• Gene Summary: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame segment in the 5' coding region and uses a different segment for its 3' coding region and UTR, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1.