Klhl8 (NM_178741) Mouse Untagged Clone

CAT#: MC219895

Klhl8 (untagged) - Mouse kelch-like 8 (Drosophila) (Klhl8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhl8
• Synonyms: 2310001P09Rik; D5Ertd431e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219895 representing NM_178741
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCAGAATCTACCAATGGGAAACAAGCTAGGAGTCACGTGACAAAGGGGCGGCGTCAGTACCAGC
  ACCAGCACCAACAGCAGCAGCAACAGCAGCAGCAAGTGAGGAGCAGGTCCTCTGTCAGTGAGTGTGATGG
  CGACGACTCCTTCATCTTCGAAGCCAACGAGGCCTGGAAGGACTTTCACGGCTCTCTCCTGGGCTTCTAT
  GAAAACGGGGAGCTCTGCGACGTCACCCTGAAGGTTGGCTCAAAGCTAATATCTTGTCACAAACTGGTGC
  TGGCTTGTGTGATCCCCTACTTCAGAGCCATGTTTCTTTCCGAAATGTCTGAAGCCAAGCAGGCGTTAAT
  CGAGATCCGAGACTTTGACGGTGATGCCGTGGAAGACTTGGTGAAGTTTGTCTACTCCTCACGGCTCACT
  TTAACAGTGGACAATGTCCAGCCCCTGCTGTATGCAGCGTGTATTCTGCAGGTTGAGCTGGTGGCCAGAG
  CTTGTTGTGAATACATGCAGCTACACTTTCATCCCTCCAACTGCCTGGCTGTACGAGCCTTCGCAGAGAG
  TCACAACCGAATAGACCTAATGGACATGGCGGACCAGTATGCCTGTGAGCACTTTACTGAAGTGGTGGAG
  TGCGAAGACTTCGTGAGTGTGTCCCCGCAACATCTCCACAAGCTCCTCTCCTCTAGCGACCTCAACATTG
  ACAGTGAGAAGCAGGTCTACAGTGCTGCCATCAAGTGGCTTCTTGCCAATCCGCAACATCATCCTAAATG
  GCTGGACGAGACACTTGCACAGGTCCGTCTGCCATTGCTGCCAGTTGATTTTCTCATGGGTGTTGTGGCG
  AAAGAACAGATCGTCAAGCAGAATCTGAAGTGCAGAGATCTGCTGGACGAAGCGAGAAATTACCACCTTC
  ACTTGAGCAGTAAACCAGTCCCGGACTTTGAGTACACAGTCAGGACGACCCCGAGGAAGCACACTGCTGG
  TGTGCTGTTTTGTGTGGGTGGTCGAGGTGGATCTGGTGACCCCTTCCGTAGTATTGAATGCTATTCTATC
  AACAAAAACAGCTGGTTCTTTGGACCAGAAATGAACAGCCGCAGGCGACATGTGGGTGTGATCTCTGTGG
  AAGGGAAGGTGTACGCAGTGGGTGGCCATGATGGGAACGAACACCTGGGCAGCATGGAGATGTTTGACCC
  TCTCACTAACAAGTGGATGATGAAGGCATCGATGAACACCAAGAGGCGAGGAATTGCCTTGGCCTCCTTA
  GGGGGCCCAATATATGCAATTGGAGGATTAGATGACAATACGTGCTTCAGTGACGTGGAGAGATACGACA
  TCGAATCTGACCAATGGAGCACAGTGGCACCAATGAACACTCCCCGTGGAGGCGTTGGCTCTGTGGCTTT
  AATAAACCACGTTTACGCAGTGGGCGGCAACGACGGCGTAGCCTCCTTATCCAGTGTGGAGAGGTATCAC
  CCGCATCTGGACAAGTGGATAGAAGTGAAAGAGATGGGTCAGCGCAGAGCTGGCAACGGCGTCAGCGAGC
  TTCACGGTTGTTTATATGTTGTTGGTGGTTTTGACGACAACTCCCCTTTGAGTTCCGTGGAACGGTACGA
  CCCTCGAAGCAACAAGTGGGACTATGTGGCAGCTCTTACCACTCCCAGGGGTGGCGTAGGGATCGCAACA
  GTGATGGGCAAAATCTTTGCAGTTGGCGGTCACAATGGCAACGCGTACCTAAACACCGTGGAGGCGTTTG
  ACCCCGTGCTGAACAAGTGGGAGCTGGTCGGACCTGTGTCTCACTGCCGAGCTGGTGCCGGTGTGGCCGT
  GTGTGACTGTTTAACTAGCCAGATCCGAGACGTGGGTCACGGGTCCACTAATGTGGTTGACTGTATGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_178741
• Insert Size: 1890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178741.3, NP_848856.1
• RefSeq Size: 3163 bp
• RefSeq ORF: 1890 bp
• Locus ID: 246293
• UniProt ID: P59280
• Cytogenetics: 5 50.45 cM
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for The BCR(KLHL8) ubiquitin ligase complex mediates ubiquitination and degradation of RAPSN.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein.