Clpx (NM_001044389) Mouse Untagged Clone

CAT#: MC219879

Clpx (untagged) - Mouse caseinolytic peptidase X (E.coli) (Clpx), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clpx
• Synonyms: AU014732; E330029I21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219879 representing NM_001044389
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGTTGCGGCGCTTGTACCTGCGGCGCTGCGGCCGCCCGGCTTCTCACCACTTCGCTCACCTCCG
  CGCAGAGAGGTATTTCGTGTGGGCGAATCCATGTGCCAGTGTTAGGAAGACTGGGGACGACCTTGGACGC
  TCAGGCTCTGCGCAGAGCTCCTCTTAGAACCTTTTCAGAAACACCAGCATACTTTGCCTCAAAAGACGGG
  GCAAACAAGGATGGCTCCGGAGATGGAAATAAGAAATCAGTGACTGAAGGAAGCAGTAAGAAATCAGGCT
  CTGGGAATTCTGGGAAAGGTGGAAACCAGCTTCGCTGTCCTAAATGTGGTGATTTGTGTACACACGTGGA
  GACCTTTGTGTCTTCCACGCGTTTTGTTAAGTGTGAAAAATGTCATCATTTTTTTGTGGTGCTGTCGGAA
  GCAGACTCAAAGAAAAGCATAATTAAGGAACCCGAGTCCGCTGCAGAGGCTGTGAAGTTGGCATTCCAGC
  AGAAGCCACCTCCGCCCCCCAAAAAGATTTATAACTACCTCGACAAGTATGTTGTTGGCCAGTCGTTTGC
  TAAGAAAGTGCTTTCAGTTGCCGTGTATAATCATTATAAGAGAATATATAATAATATTCCAGCTAATCTG
  AGACAGCAAGCAGAGGCTGAGAAGCAGACATCACTAACACCTAGAGAGTTGCTTCAGATCGCTGGGATCA
  GCCCACACGGCAATGCCTTGGGAGCCTCTATGCAGCAGCAGGTAAACCAGCAAATGCCTCAGGAGAAAAG
  AGGAGGTGAAGTGTTGGACTCTTCTCAAGATGATATAAAACTGGAAAAAAGTAATATTTTGCTGCTTGGA
  CCAACTGGGTCAGGTAAAACCCTTCTGGCACAAACTTTAGCTAAATGCCTTGATGTTCCTTTTGCTATCT
  GTGACTGTACAACTTTGACCCAGGCTGGATATGTAGGTGAAGATATTGAATCTGTGATTGCCAAACTACT
  CCAAGATGCCAATTACAATGTAGAGAAAGCACAACAAGGAATTGTCTTTCTGGATGAAGTAGATAAGATT
  GGCAGTGTGCCAGGCATTCATCAATTACGGGATGTAGGTGGAGAAGGCGTTCAGCAAGGTTTATTAAAAC
  TTCTAGAAGGCACAATAGTCAATGTTCCAGAAAAGAATTCTCGAAAACTACGTGGAGAGACAGTTCAAGT
  TGATACAACAAATGTCTTGTTTGTGGCATCTGGTGCCTTTAATGGCTTAGACAGAATCATCAGCAGGAGG
  AAAAATGAAAAGTATCTCGGCTTTGGAACACCATCTAACCTGGGAAAGGGCAGAAGGGCTGCAGCTGCAG
  CGGATCTTGCTAACCGAAGTGGGGAATCTAACACTCATCAGGACATTGAGGAGAAAGACCGGTTACTCCG
  CCATGTGGAAGCCAGAGATCTTATTGAGTTTGGCATGATACCAGAGTTTGTGGGACGGTTGCCTGTGGTG
  GTTCCTTTGCACAGCCTAGATGAGAAGACACTTGTACAAATACTGACCGAGCCACGGAATGCTGTTATTC
  CTCAGTATCAGGCCTTGTTCAGCATGGATAAGTGTGAGCTGAATGTTACTGAGGATGCTCTGAAAGCCAT
  AGCGAGATTGGCCCTGGAAAGAAAAACAGGTGCACGAGGCCTTCGGTCTATAATGGAAAAGCTGTTACTA
  GAACCAATGTTCGAAGTTCCTAATTCTGACATTGTATGTGTGGAAGTTGACAAAGAAGTAGTAGAAGGCA
  AAAAGGAACCAGGATACATTCGGGCACCCTCAAAAGAGTCCTCTGAAGAGGAATATGACTCTGGAGTGGA
  GGAGGATGGATGGCCTCGTCAAGCGGATGCTGCAAACAGCTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001044389
• Insert Size: 1863 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001044389.2, NP_001037854.1
• RefSeq Size: 2844 bp
• RefSeq ORF: 1863 bp
• Locus ID: 270166
• Cytogenetics: 9 35.22 cM
• Gene Summary: ATP-dependent specificity component of the Clp protease complex. Hydrolyzes ATP. Targets specific substrates for degradation by the Clp complex. Can perform chaperone functions in the absence of CLPP. Enhances the DNA-binding activity of TFAM and is required for maintaining a normal mitochondrial nucleoid structure (PubMed:10347188). ATP-dependent unfoldase that stimulates the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis. Important for efficient erythropoiesis through upregulation of heme biosynthesis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.