Pias3 (NM_001165949) Mouse Untagged Clone

CAT#: MC219868

Pias3 (untagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pias3
• Synonyms: Pias3l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219868 representing NM_001165949
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGGGCGAGTTAAAGCACATGGTGATGAGTTTCCGAGTGTCTGAGCTCCAGGTGCTCCTCG
  GCTTCGCTGGCAGGAACAAGAGTGGGCGGAAACACGAGCTGCTGGCCAAGGCCCTGCACCTCCTCAAGTC
  TAGCTGCGCCCCCAGCGTCCAGATGAAGATCAAAGAACTTTATCGCAGGCGCTTTCCCCGGAAGACCCTG
  GGGCCTTCTGATCTCTCCTTGCTTTCTTTGCCCCCTGGCACCTCTCCTGTAGGCTCCCCCGGCCCCCTTG
  CTCCCATCCCTCCCACCCTTCTGACCCCTGGCACCTTGCTAGGCCCTAAGCGTGAGGTGGACATGCATCC
  TCCTCTGCCGCAGCCTGTGCACCCCGATGTCACCATGAAGCCACTGCCCTTCTATGAAGTCTATGGGGAG
  CTCATCCGACCCACCACCCTTGCGTCCACCTCCAGCCAGAGGTTCGAGGAAGCCCACTTCACCTTCGCGC
  TCACTCCCCAGCAGCTGCAGCAGATTCTCACGTCCAGGGAGGTTCTGCCAGGAGCCAAGTGTGATTACAC
  CATACAAGTGCAGCTCAGATTCTGTCTCTGTGAGACCAGCTGCCCTCAGGAGGACTATTTCCCCCCTAAC
  CTCTTTGTTAAGGTTAATGGGAAACTCTGCCCCCTGCCGGGTTACCTCCCTCCAACCAAGAATGGAGCTG
  AGCCCAAGAGGCCCAGCCGTCCGATCAACATCACACCCTTGGCTCGACTCTCAGCCACTGTCCCCAACAC
  CATCGTAGTTAATTGGTCATCTGAGTTTGGACGGAATTACTCCTTGTCCGTGTACCTGGTGAGGCAATTG
  ACTGCAGGGACCCTTCTACAAAAACTCAGAGCCAAGGGGATCCGGAATCCAGACCATTCCCGGGCACTGA
  TCAAGGAGAAACTGACTGCTGACCCCGACAGTGAAGTGGCTACTACAAGTCTCCGGGTGTCACTCATGTG
  CCCGCTAGGGAAGATGCGCCTGACTGTCCCGTGTCGTGCCCTCACCTGTGCCCATCTGCAGAGTTTCGAT
  GCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCGACATGGACGTGTCCTGTGTGTGACAAGAAGGCTC
  CCTATGAGTCGCTGATTATTGATGGTTTATTCATGGAAATTCTTAATTCCTGTTCGGATTGTGATGAGAT
  CCAGTTCATGGAAGATGGATCCTGGTGTCCGATGAAACCCAAGAAGGAGGCATCAGAGGTTTGCCCCCCG
  CCAGGGTATGGGCTGGATGGTCTCCAGTACAGCGCAGTCCAGGAGGGAATTCAGCCAGAGAGTAAGAAGA
  GGGTCGAAGTCATTGACTTGACCATCGAAAGCTCATCAGATGAGGAGGATTTGCCCCCCACCAAGAAGCA
  CTGCCCTGTCACCTCAGCGGCCATTCCAGCCCTTCCTGGAAGCAAAGGAGCCCTGACCTCTGGTCACCAG
  CCATCCTCGGTGCTGCGGAGCCCTGCAATGGGCACACTGGGCAGTGACTTCCTGTCTAGTCTCCCGCTAC
  ATGAGTACCCACCTGCCTTCCCACTGGGGGCTGACATCCAAGGTTTAGATTTATTTTCTTTCCTTCAGAC
  TGAGAGTCAGCACTACGGCCCTTCAGTTATCACTTCGCTAGATGAACAGGACACCCTTGGCCACTTCTTC
  CAGTACCGGGGAACCCCTTCCCACTTCCTGGGCCCACTGGCCCCCACACTGGGGAGCTCTCACCGCAGCT
  CCACTCCAGCGCCCCCTCCTGGTCGTGTCAGCAGCATTGTGGCTCCTGGGAGCTCCTTGAGGGAAGGGCA
  TGGAGGACCCCTGCCTTCAGGTCCCTCTTTGACTGGCTGTCGGTCAGACGTCATTTCCTTGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001165949
• Insert Size: 1887 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165949.1, NP_001159421.1
• RefSeq Size: 2938 bp
• RefSeq ORF: 1887 bp
• Locus ID: 229615
• UniProt ID: O54714
• Cytogenetics: 3 F2.1
• Gene Summary: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Repressor of STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Repressor of MITF transcriptional activity. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1 (By similarity). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and uses an alternate translational start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is longer than isoform 1.