Ttc25 (NM_028918) Mouse Untagged Clone

CAT#: MC219841

Ttc25 (untagged) - Mouse tetratricopeptide repeat domain 25 (Ttc25), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttc25
• Synonyms: 4933404O19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219841 representing NM_028918
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACGGTGATATCTTAAGAAGCACTTTTCCCTCTTACATGGCAGAGGGGGAGAGGCTTTATCTGT
  GCGGGGAGTTCACTAAGGCCATTCAAAGCTTTACCAACGCTCTTCACCTTCAAAGTGGAGATAAGAACTG
  CCTGGTTGCCCGCTCCAAATGCTACCTGAAGATGGGAGACTTAGAGAAGTCCTTGAACGATGCTGAGGCC
  TCACTTCGGAACGACCCGACTTTCTGCAAGGGGATCTTACAGAAGGCTGAGACACTGTACACCATGGGAG
  ACTTTGAGTTTGCCCTGGTGTTCTATCATCGAGGCTACAAACTGAGACCTGATCGGGAATTCAAAGTAGG
  AATTCAGAAGGCCCAGGAAGCCATCAACAACTCGGTGGGAAGTCCTTCTTCAATCAAGCTGGAGAACAAA
  GGCGACCTCTCCTTCTTAAGCAAGCAAGCTGAGAGTAAGAAAGCCCAACAGAAGCATCTGCCTATAAAGC
  AACTCTCGTACAGCACCAAGCATGAAATCAAGCGGAAGGGCTCGCTCAAGAGCGAGAAGACCGTGCGGCA
  GCTGCTCGGGGAGCTGTACGTGGACAAGGAGTATCTGGAGAAGCTCCTTCTGGATGAAGATCTCATCAAA
  GGCACCATCAAGAGTGGCCTGACCGTGGAGGACCTCATTATGACTGGCATCAACTACCTGGACACCCGCA
  GCAACTTCTGGAGGCAGCAGAAGCCCATCTATGCCAGGGAGCGAGACCGGAAGCTGATGCAAGAGAAGTG
  GCTGAGGGACCGGAAACGCAGCCCCTCGCAGACAGCCCACTACATCCTCAAGAGCCTAGAGGACATTGAC
  ATGTTGTTGACCAGTGGCAGTGCTGATGGGAGCCTCCAAAAAGCTGAGAAAGTCCTTAAGAAGGTTCTGG
  AATGGAACCAAGAGGAGGTACCCAACAAGGACGAGCTGGTCGGGAACTTGTACAGCTGCATAGGCAACGC
  CCAGATTGAGCTGGGGCAGATGGTGGCTGCCTTGCAGAGCCACCGAAAGGACCTGGAGATCGCCAAGGAA
  CATGACCTTCCAGATGCAAAATCGAGAGCCCTTGACAATATTGGCAGAGTTTTTGCCAGAGTTGGGAAAT
  TCCAGCAAGCCATTGACACGTGGGAGGAGAAGATCCCTCTAGCCAAAACCACCCTGGAGAAGACCTGGCT
  CTTCCACGAGATTGGCCGCTGTTACTTGGAGCTGGACCAGGCGTGGCAGGCCCAGAGTTATGGGGAAAAG
  TCTCAGCAGTACGCTGAGGAGGAAGGGGACCTCGAGTGGCAGCTGAACGCCAGTGTCCTGGTGGCGCAGG
  CCCAAGTGAAGCTGAGAGACTTCGAGTCAGCTGTGAATAACTTTGAGAAGGCCCTGGAGAGAGCCAAGCT
  GGTCCACAACAACGAGGCGCAGCAGGCCATCATCAGCGCCTTGGACGATGCCAACAAGGGCATCATCGAA
  GAACTTAAGAAAACGAACTACAGGGAGATACTCAGGGAAAAGGCAGAGAGACAGGACATCATGTCACAGA
  TGGATTTGCAGGGAGCATCTGAGAAGGAGCCGCTGCGAGGGCGAGAGGAGCAGGAGAGGGTGGTGAAGCA
  GTGGGAGAGGGACCAGGAAAGTGAGAGAGAAGCCACGGATGACGAGCAAGACAGGAAAAGCTCGGGAGGA
  CTCAGCAAAAAACTGTTGGGAGACGGTCACTCGTCAAACCTAGGGATTAGGAGAGAGTCCAGAGAAATCT
  ACAGAAGGCTCTCGGACTACTCCAGCCACCTACCCTCAGAGGACGGCAGCCAAAAACAAGAGAAGAAACA
  AGCAGAAGCAGCCAAAGGGGAGGTCCAAAAGTTGGAGAAGACAAAAGAGGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028918
• Insert Size: 1875 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028918.2, NP_083194.2
• RefSeq Size: 2818 bp
• RefSeq ORF: 1875 bp
• Locus ID: 74407
• UniProt ID: Q9D4B2
• Cytogenetics: 11 D
• Gene Summary: Required for the docking of the outer dynein arm to cilia, hence plays an essential role in cilia motility.[UniProtKB/Swiss-Prot Function]