Sh3kbp1 (NM_021389) Mouse Untagged Clone

CAT#: MC219829

Sh3kbp1 (untagged) - Mouse SH3-domain kinase binding protein 1 (Sh3kbp1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sh3kbp1
• Synonyms: 1200007H22Rik; 1700125L08Rik; 5830464D22Rik; AI447724; Cin85; IN85; Ruk; Seta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219829 representing NM_021389
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTTTCTGCAGCCAAAGCCCCAAGCCCCACAGACTTGCCTGAGAGTGAAATAAAGAAAGATATGA
  AGAAAGATCTTCTCTCTAACAAAGCTCCAGAGAAACCCATGCACGATGTATCCAGTGGAAACGCTCTGCT
  TTCTTCTGAAACCATTTTAAGAACCAATAAACGAGGAGAACGACGGAGACGCCGATGCCAGGTGGCATTC
  AGCTACCTGCCCCAGAATGACGATGAGCTGGAGCTGAAAGTCGGGGACATCATAGAGGTTGTAGGAGAGG
  TAGAGGAAGGATGGTGGGAAGGTGTGCTCAATGGGAAGACTGGAATGTTTCCTTCCAACTTCATCAAGGA
  GCTGTCAGGGGAGTCGGATGAGCTTGGCATCTCCCAGGATGAGCAGCTTTCCAAGTCAAGCTTAAGAGAA
  ACCACAGGCTCCGAGAGTGATGGAGGCGACTCCAGCAGTACCAAGTCCGAAGGTGCCAACGGGACCATGG
  CAACTGCAGCCATCCAGCCCAAGAAAGTTAAAGGAGTGGGGTTTGGAGACATTTTCAAAGACAAGCCAAT
  TAAACTAAGACCAAGGTCGATTGAAGTGGAAAATGACTTCCTGCCCGTCGAAAAGACAATTGGGAAGAAG
  TTACCTCCAGCTACATCAACTCCAGACCCATCGAAGACGGAGATGGACAGCAGGACAAAGACCAAGGATT
  ACTGCAAAGTAATATTTCCATATGAGGCACAGAATGATGATGAATTGACAATCAAGGAAGGAGATATAGT
  GACTCTCATCAATAAGGACTGCATCGACGTAGGCTGGTGGGAAGGAGAACTCAATGGCAGACGAGGCGTG
  TTTCCTGACAACTTCGTGAAGTTACTTCCATCGGACTTTGACAAGGAAGGGAATAGACCCAAGAAACCGC
  CACCTCCATCAGCTCCTGTCGTCAAACAAGGGGCAGGTACCACAGAGAGAAAACATGAAATTAAAAAAAT
  ACCTCCTGAAAGACCAGAAACCCTTCCAAACAGAACAGAAGAGAAAGAAAGACCAGAGAGAGAGCCAAAA
  CTGGATTTACAGAAGCCTTCAGTTCCTGCCATCCCACCAAAAAAGCCTCGGCCACCTAAGACCAATTCTC
  TGAACAGACCTGGTGCATTGCCCCCAAGAAGACCTGAGCGACCAGTGGGCCCACTGACACACACCAGGGG
  TGACAGTCCAAAGATTGACTTGGCAGGCAGCGCACTCTCTGGCATCCTGGATAAGGACCTCTCAGACCGC
  AGCAATGATATTGACTTAGAAGGTTTTGACTCTGTGATATCATCTACTGAGAAACTCAGCCACCCAACCA
  CAAGCAGACCAAAAGCTACAGGAAGACGGCCTCCATCCCAGTCACTCACATCTTCTTCCCTTTCAAGCCC
  CGATATCTTTGACTCCCCAAGTCCCGAAGAAGATAAAGAGGAACACATTTCGCTTGCGCACAGAGGAATA
  GACGTGTCAAAGAAGACTTCCAAGACTGTTACCATATCCCAAGTGTCTGATAACAAGACATCCCTGCCAC
  CCAAGCCAGGGACCATGGCAGCTGCCAGCAGTGGGCCAGCTTCTCTCTCTTCAGTGGCATCCTCACCCAT
  GTCATCCTCTTTGGGAACAGCTGGACAGAGAGCCAGTTCTCCATCTCTGTTCAGCACAGAAGGAAAGCCA
  AAGATGGAGCCAGCAGTGAGCAGCCAGGCTGCTATCGAGGAGCTTAAGATGCAAGTCCGTGAGCTGAGGA
  CCATCATTGAGACCATGAAGGACCAGCAGAAACGTGAGATTAAGCAGTTACTGTCAGAATTGGATGAAGA
  GAAAAAGATCCGGCTCCGGTTGCAGATGGAAGTGAACGACATAAAGAAAGCTCTTCAATCAAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021389
• Insert Size: 1887 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021389.6, NP_067364.2
• RefSeq Size: 4733 bp
• RefSeq ORF: 1887 bp
• Locus ID: 58194
• UniProt ID: Q8R550
• Cytogenetics: X F4
• Gene Summary: Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may be inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2, also known as RukdeltaA) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.