Ngef (NM_019867) Mouse Untagged Clone

CAT#: MC219824

Ngef (untagged) - Mouse neuronal guanine nucleotide exchange factor (Ngef), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ngef
• Synonyms: ephexin; Tims2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219824 representing NM_019867
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCTGGCTGCAGCCTTCAGCGCCGCCTGCGCCGTGGACCACGACAGCTCCACCTCGGAGAGCG
  ACACGCGCGACTCGGCGGCGGGACACCTGCCGGGCAGCGAGTCATCCTCCACCCCTGGAAATGGAACCAC
  ACCCGAGGAGTGCCCAGCCCTCACCGACAGCCCCACCACTCTCACGGAGGCCCTGCAGATGATCCATCCC
  ATTCCCGCCGACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAAGAGTATAGAGACAAAT
  CGACTCTCCAAGAAATTGAAACACGGAGGCAGCAGGATGCAGAAATCCAAGGCAACTCCGATGGGTCCCA
  GGTTGGGGAAGACGCTGGAGAGGAGGAGGAGGAGGAGGAGGAGGGAGAGGAGGAGGAGCTGGCCAGCCCT
  CCTGAGAGGAGAGCTCTGCCTCAGATCTGCCTGCTCAGCAACCCCCACTCCAGGTTCAACCTCTGGCAAG
  ACCTTCCTGAGATCCAGAGCAGTGGCGTGCTGGACATTCTCCAGCCGGAGGAGATCAGGCTGCAGGAGGC
  CATGTTTGAGTTGGTTACCTCTGAGGCCTCCTACTATAAGAGCCTGAACCTGCTGGTGTCGCACTTCATG
  GAGAACGAGCGTCTGAAGAAGATCCTGCATCCATCTGAGGCCCACATCCTCTTTTCCAATGTCCTGGATG
  TCATGGCTGTCAGTGAGCGGTTTTTGCTGGAGCTAGAGCACCGCATGGAGGAGAACATTGTTATCTCGGA
  TGTGTGCGACATCGTGTACCGTTACGCAGCTGATCACTTCTCGGTCTATATCACTTACGTCAGTAACCAG
  ACCTACCAGGAAAGGACATACAAGCAGCTCCTACAGGAGAAGGCCGCTTTCCGGGAACTGATCGCACAGT
  TGGAGCTGGACCCCAAATGCAAGGGCCTGCCTTTCTCCTCCTTCCTCATCTTGCCTTTCCAGAGGATCAC
  GAGACTCAAGCTGCTGGTCCAGAATATCCTGAAGAGAGTGGAGGAGAGGTCTGAACGTGAAGGCACCGCC
  TTGGATGCCCACAAGGAGCTAGAAATGGTGGTAAAGGCATGCAATGAGGGTGTCCGGAAGATGAGCCGCA
  CAGAACAGATGATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTACCCATCATCTCACACTC
  CCGGTGGCTGCTGAAGCAGGGTGAGCTGCAGCAGATGTCCGGCCCCAAGACCTCCCGCACCCTGCGGACC
  AAGAAGCTCTTCAGAGAAATTTACCTCTTCCTCTTCAATGACCTGCTGGTGATCTGCCGGCAGATCCCTG
  GAGACAAGTACCAGGTGTTTGATTCGGCCCCAAGGGGCCTGCTTCGAGTGGAGGAGCTGGAGGACCAGGG
  TCAAACACTGGCTAATGTGTTCATCCTGCGGCTGCTGGAAAATGCAGATGACCGAGAGGCCACCTATATG
  CTGAAGGCATCCTCCCAGAGCGAGATGAAGCGCTGGATGACCTCACTGGCCCCCAACAGGAGGACCAAGT
  TTGTATCCTTCACATCTCGGCTGTTGGACTGTCCCCAGGTCCAGTGTGTGCACCCGTATGTGGCCCAGCA
  GCCTGATGAACTGACGCTGGAACTGGCAGATATCCTGAACATCCTGGAGAAGACAGAGGATGGGTGGATC
  TTTGGTGAGCGGCTGCATGACCAGGAGAGAGGCTGGTTCCCCAGTTCCATGACAGAGGAGATCCTGAACC
  CCAAGATCCGCTCCCAGAACCTCAAGGAATGTTTCCGGGTACATAAGATGGAAGACCCTCAGCGCAGCCA
  GAATAAGGACCGCAGGAAGCTGGGCAGCCGGAATCGTCAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019867
• Insert Size: 1863 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019867.2, NP_063920.2
• RefSeq Size: 2764 bp
• RefSeq ORF: 1863 bp
• Locus ID: 53972
• UniProt ID: Q8CHT1
• Cytogenetics: 1 44.42 cM
• Gene Summary: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains a different segment for its 5' UTR and 5' coding region, and lacks other coding region exons, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct N-terminus when it is compared to isoform 1.