Dnajc2 (NM_009584) Mouse Untagged Clone

CAT#: MC219814

Dnajc2 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 2 (Dnajc2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnajc2
• Synonyms: AU020218; MIDA1; Zrf1; Zrf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219814 representing NM_009584
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCCTGCCGAGCGCCGCGGAGGGCCAGGGCACCGCCATCACCCACGCTCTGACCTCCGCCTCTT
  CAGTCTGTCAAGTTGAACCTGTGGGAAGATGGTTTGAAGCTTTTGTTAAGAGGAGAAACAGAAATGCTTC
  TACATCTTTTCAGGAACTGGAAGATAAGAAAGAGTTGTCAGAGGAATCAGAAGATGAAGAGTTACAGTTA
  GAAGAATTCCCCATGCTGAAAACACTTGATCCCAAAGATTGGAAGAATCAAGATCATTATGCGGTTCTTG
  GACTTGGCCACGTCAGATACACAGCTACACAGAGACAGATCAAAGCAGCTCATAAAGCAATGGTCTTGAA
  ACATCATCCAGACAAACGGAAAGCGGCTGGGGAACCCATCAAAGAAGGAGACAATGACTACTTCACTTGC
  ATAACTAAAGCTTATGAAATGTTGTCTGATCCAGTGAAAAGGCGGGCATTTAACAGTGTAGATCCTACTT
  TTGACAACTCAGTTCCTTCTAAAAGTGAAGCTAAGGACAATTTTTTTCAAGTGTTTTCTCCAGTGTTTGA
  AAGGAATTCAAGATGGTCAAATAAAAAAAATGTTCCCAAGCTTGGTGATATGAACTCCTCGTTTGAAGAT
  GTAGATGCGTTTTACTCTTTCTGGTATAATTTTGATTCTTGGAGAGAATTTTCTTATTTAGATGAAGAAG
  AAAAAGAAAAAGCAGAATGCCGTGATGAGAGGAAGTGGATTGAAAAGCAGAACAGAGCCACGAGGGCACA
  AAGGAAAAAGGAAGAGATGAACAGAATAAGAACATTAGTTGATAATGCTTACAGTTGTGACCCAAGGATA
  AAAAAATTTAAGGAAGAAGAAAAGGCCAAGAAAGAAGCAGAAAAGAAAGCAAAAGCAGAGGCCAGGCGGA
  AGGAGCAGGAAGCCAAAGAAAAGCAAAGACAAGCTGAATTAGAAGCTGTTCGGTTGGCTAAGGAGAAAGA
  AGAGGAAGAAGTTAGACAGCAAGCATTGTTGGCCAAGAAGGAAAAAGATATCCAGAAGAAAGCCATTAAA
  AAGGAAAGGCAGAAACTTCGGAACTCGTGCAAGAGCTGGAATCACTTCTCGGACAATGAGGCAGACCGTG
  TTAAAATGATGGAAGAGGTGGAAAAACTCTGTGATCGGCTTGAACTAGCAAGCTTGCAGGGCTTGAATGA
  AATCCTGGCTTCATCTACGAGAGAAGTAGGAAAAGCTGCACTTGAAAAACAGATAGAAGAAGTGAATGAG
  CAGATGAGGAGAGAGAAGGAGGAAGCTGATGCTCGGATGCGGCAAGCATCCAAGAATGCAGAGAAGTCAA
  CTGGTGGAAGTGGGAGTGGCAGTAAAAATTGGTCAGAAGATGATCTGCAATTATTAATTAAAGCTGTCAA
  TCTCTTCCCTGCTGGAACAAACTCAAGATGGGAAGTCATTGCTAATTATATGAACATACATTCTTCTTCT
  GGCGTCAAAAGAACTGCCAAAGATGTTATTAGCAAGGCTAAAAGCCTCCAAAAACTTGACCCTCATCAAA
  AAGATGACATTAACAAAAAGGCTTTTGATAAGTTTAAAAAAGAACATGGAGTGGCCTCTCAGGCTGACAG
  TGCAGCACCATCAGAACGGTTTGAAGGTCCCTGCATAGATTCCACCCCTTGGACAACAGAAGAACAGAAA
  CTTTTAGAACAGGCTTTGAAAACATACCCAGTAAATACTCCTGAACGATGGGAAAAGATAGCCGAAGCTG
  TTCCTGGCAGGACGAAGAAGGACTGCATGAGGCGATACAAGGAACTTGTGGAGATGGTGAAAGCCAAGAA
  AGCTGCTCAGGAGCAAGTGCTGAATGCAAGCAGAGCCAGGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009584
• Insert Size: 1866 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052027, AAH52027
• RefSeq Size: 2086 bp
• RefSeq ORF: 1866 bp
• Locus ID: 22791
• UniProt ID: P54103
• Cytogenetics: 5 9.97 cM
• Gene Summary: Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation (By similarity). Specifically binds DNA sequence 5'-GTCAAGC-3'.[UniProtKB/Swiss-Prot Function]