Dtx1 (NM_008052) Mouse Untagged Clone

CAT#: MC219798

Dtx1 (untagged) - Mouse deltex 1 homolog (Drosophila) (Dtx1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dtx1
• Synonyms: Fxit1; mKIAA4160
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219798 representing NM_008052
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACGGCCAGGCCAAGGTGTGATGGTGCCGGTGAACGGGCTGGGCTTCCCGCCGCAGAACGTGGCCA
  GGGTGGTGGTGTGGGAGTGGCTCAATGAGCACAGCCGCTGGCGACCCTACACGGCCACCGTGTGCCACCA
  CATCGAGAATGTTCTTAAGGAGGATGCCCGGGGCTCTGTGGTCCTTGGCCAGGTGGACGCCCAGCTGGTG
  CCCTACATCATCGACCTACAATCCATGCATCAGTTCCGGCAAGACACAGGTACCATGCGGCCGGTGCGAC
  GCAACTTCTACGATCCATCGTCGGCGCCGGGCAAGGGCATCGTGTGGGAATGGGAGAACGACGGCGGGGC
  GTGGACGGCCTACGACATGGACATCTGCATCACCATCCAGAACGCGTACGAGAAGCAGCACCCGTGGCTC
  GACCTCTCATCGCTCGGCTTCTGCTACCTCATCTACTTCAACAGCATGTCCCAGATGAACCGCCAGACGC
  GCCGCCGCCGCCGCCTGCGCCGTCGCCTGGACCTGGCTTACCCGCTCACTGTCGGCTCCATTCCCAAGTC
  GCAATCCTGGCCCGTGGGAGCCAGCTCGGGTCAGCCCTGCTCCTGTCAGCAGTGCCTGCTGGTCAACAGC
  ACGCGCGCCGCCTCCAACGCCATCCTGGCCTCGCAGCGCCGCAAGGCTCCCATTGCGCCAGCCGCGCCTC
  CAGCGCCCCCTCCGCCCCCGCCGCCGCTGCCACCCGGAGGACCTCCGGGTGCGCTCGTTGTGCGCCCCAG
  CGCCACTTTCGCCGGAGCTGCGCTCTGGGCCGCACCTGCCACCGGCCCCACGGAGCCTGCGCCGCCTCCA
  GGAGTTCCCCCAAGGAGCCCTAGTGCCCCCAACGGAGCGCCCACACCGGGCCAAAACAACCTCAGTCGAC
  CAGGACCACAGAGGTCCACCAGCGTCAGCGCACGCGCCTCTATCCCGCCTGGGGTTCCGGCGCTCCCCGT
  GAAGAACTTGAATGGCACTGGCCCTGTCCACCCAGCCTTGGCAGGGATGACCGGGATCCTGCTGTGTGCA
  GCGGGGCTGCCGGTGTGCCTGACACGAGCACCCAAACCCATCCTGCACCCACCACCAGTAAGCAAAAGCG
  ACGTGAAGCCTGTGCCTGGAGTGCCCGGCGTGTGCCGCAAGACCAAGAAGAAACACCTCAAGAAAAGCAA
  GAATCCTGAGGATGTGGTTCGGAGGTACATGCAGAAGGTGAAAAACCCGCCTGATGAGGACTGTACCATT
  TGCATGGAGCGGCTGGTCACAGCATCTGGCTATGAGGGCGTGCTCCGAAACAAGAGTGTGCGGCCCGAGC
  TTGTGGGCCGCCTGGGCCGCTGCGGCCACATGTATCACCTGCTCTGCCTGGTGGCCATGTACTCCAATGG
  CAACAAGGATGGCAGCCTGCAGTGTCCAACCTGCAAAGCCATCTACGGGGAGAAGACAGGGACACAGCCA
  CCAGGGAAGATGGAGTTTCACCTCATCCCGCACTCGCTGCCTGGTTTTGCAGACACCCAGACGATCCGCA
  TCGTCTATGACATCCCCACGGGCATCCAGGGCCCTGAACATCCCAACCCAGGCAAGAAGTTCACAGCCAG
  AGGCTTCCCTCGCCACTGCTACCTACCCAACAATGAGAAGGGCCGAAAGGTGCTGAGATTGCTCATCACC
  GCCTGGGAACGCAGACTCATCTTCACTATCGGAACATCCAACACCACGGGCGAGTCGGACACCGTGGTGT
  GGAACGAGATTCACCACAAGACGGAGTTTGGTTCCAACCTCACTGGTCACGGCTACCCCGACGCCAGCTA
  CCTAGACAACGTGCTGGCTGAGCTCACCGCCCAGGGGGTTTCTGAGGCCATGGCCAAGGCCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_008052
• Insert Size: 1884 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008052.3, NP_032078.2
• RefSeq Size: 3715 bp
• RefSeq ORF: 1884 bp
• Locus ID: 14357
• UniProt ID: Q61010
• Cytogenetics: 5 60.64 cM
• Gene Summary: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.[UniProtKB/Swiss-Prot Function]