Lbr (NM_133815) Mouse Untagged Clone

CAT#: MC219788

Lbr (untagged) - Mouse lamin B receptor (Lbr), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lbr
• Synonyms: AI505894; ic
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219788 representing NM_133815
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGTAGGAAGTTTGTTGAGGGTGAAGTGGTCAGAGGCCGATGGCCTGGGAGCTCCCTTTATTATG
  AAGTAGAGATTCTGAGCCACGACAACAAATCCCAGCTCTACACTGTAAAATACAAAGATGGCACCGAACT
  GGAACTGAAGGAGAGTGATATCAAGCCTTTAAAATCCTTTAAGCAAAGAAAAAGTGGCTCGATTTCCAGC
  TCTCCCTCTCGACGCCGTGGCAGCCGCAGCCGCAGCCGCTCAAGATCACGTTCCCGATCCCCGGGCAGAG
  CACCGAAAGGCTCCAGAAGATCCGTCTCTGCTTCCCATGAGGGTGACGTGAAGGAGAAGAAGGAGAAGGA
  GATGAGGAGGGAAATTCTGCAAGTTAAGCTGACTCCGCTTGTGTTGAAGCCGTTCGGAAACAGCGTCAGC
  GTGTACAACGGGGAGCCCGAACACATGGAGAAGAACGCTACGCCGTATAAAGACAAGCAGGAAAGAATCA
  TTTTGTCAACAGAAGACAGATATATAGTTACACAGTACAGCCTTCGTCCCAGGAGAGAAGAGGTCAAAGC
  CAAAGAAATAGAGTCTGAGGAACAAAACCTTGTCACCAAAGGACCCGCACCTTTGGGAACCTTTCAAGTG
  ACCACTCCACAGAGGAAGGACTTGGAGTTTGGAGGAGTACCTGGTGCGGTCCTCATCATGCTGGGCCTGC
  CCGCCTGCGTCCTCTTGCTGCTGCTGCAGTGCAGGCAGAAGGACCCGGGGCTGCTCCACTTCCCTCCACC
  TCTGCCGGCTCTCCACGAGCTGTGGGAACCCAGGGTGTGCGGCGTTTACCTCCTCTGGTTTTTCGTTCAA
  GCTCTCTTCCACCTTCTACCAGTTGGGAAGGTTGCAGAAGGAACACCTCTTGTTGATGGAAGAAGACTCC
  AGTATAGACTGAATGGATTGTATGCCTTCATTCTGACATCTGCAGCCCTGGGAGCAGCTGTCTTCTGGGG
  TGTTGAGCTGTGCTACCTGTACACTCACTTCCTGCAGTTGGCACTGGCAGCCACCGGGTTCTCCGTGCTC
  CTGAGTGCTTACCTCTATGTTCGCTCTCTGCGAGCCCCGAGGGAGGAGCTGTCGCCAGCCAGTTCCGGAA
  ATGCTGTCTACGACTTCTTCATTGGCCGAGAGTTAAACCCGCGACTTGGTGCTTTCGATCTCAAGTTCTT
  TTGTGAGTTGCGCCCTGGATTGATTGGATGGGTGGTTATTAACTTAGTGATGCTTCTGATGGAAATGAAA
  ATTCAGGAGCGAGCTGCTCCGTCCTTGGCAATGATCTTGGTTAACAGTTTCCAGCTCTTATATGTGGTGG
  ACGCCCTCTGGAATGAGGAAGCACTGCTGACCTCCATGGACATCATGCACGACGGCTTTGGCTTCATGCT
  GGCCTTTGGGGATTTAGTGTGGGTTCCATTTACCTACAGCCTCCAGGCCTTTTACCTGGTCAGCCATCCC
  CATGACTTGTCCTGGCCATTGGCTTCTGTCATCATTGCTCTGAAATTGTGTGGATATGTAATCTTCCGTT
  GTGCAAACTCTCAGAAAAATGCATTCCGGAAGAATCCCACTGATCCAAAGCTTGCACATTTGAAGACCAT
  TCATACTTCCACGGGGAAAAGTCTGCTTGTTTCCGGATGGTGGGGCTTTGTTCGCCATCCCAATTACTTG
  GGTGACCTCATCATGGCTCTGGCGTGGTCCCTCCCATGTGGTTTCAACCACCTCCTGCCATACTTCTACA
  TTATCTACTTCACCGCCTTGCTTATCCATCGAGAGGCCCGGGATGAACACCAGTGCAGGAGGAAGTACGG
  CCTGGCCTGGGAGAAGTACTGCCAGCGAGTGCCCTACCGCATATTCCCCTACATTTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133815
• Insert Size: 1881 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133815.2, NP_598576.2
• RefSeq Size: 3568 bp
• RefSeq ORF: 1881 bp
• Locus ID: 98386
• UniProt ID: Q3U9G9
• Cytogenetics: 1 84.89 cM
• Gene Summary: Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis (PubMed:18785926). Plays a critical role in myeloid cell cholesterol biosynthesis which is essential to both myeloid cell growth and functional maturation (PubMed:22140257). Mediates the activation of NADPH oxidases, perhaps by maintaining critical levels of cholesterol required for membrane lipid raft formation during neutrophil differentiation (PubMed:22140257). Anchors the lamina and the heterochromatin to the inner nuclear membrane (By similarity).[UniProtKB/Swiss-Prot Function]