Vps33b (NM_178070) Mouse Untagged Clone

CAT#: MC219766

Vps33b (untagged) - Mouse vacuolar protein sorting 33B (yeast) (Vps33b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps33b
• Synonyms: MGC36556
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219766 representing NM_178070
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTTCCCCATCGGCTGGACGCCCCTGAGCTTCCCGACTTCTCTATGCTCAAGAGGCTGGCTCGGG
  ACCAGCTCATCTATCTGCTAGAACAGCTTCCTGGAAAAAAGGATTTGTTCATTGAGGCAGATCTCATGAG
  CCCTTTGGATCGAATTGCTAACGTCTCTATCCTGAAGCAACATGAAGTGGACAAGCTGTACAAGGTGGAG
  AACAAACCTGCCCTCAGCGCCAATGAACAACTGTGCTTCTTGGTCAGACCTCGAATCAAGAATATGCGCT
  ACATTGCCAGTCTTGTCAATGCTGACAAGCTGGCTGGCAGAATTAGAAAATACAAAGTCATCCTAAGCCC
  TCAGAAGTTTTATGCATGTGAGATGGTGCTTGAGGAAGAGGGAGTCTATGGAGATGTGAGCTGTGATGAG
  TGGGCCTTTTCTCTGCTGCCTCTTGATGTCGATCTGCTGAGCATGGAACTGCCAGAATTTTTCAGGGATT
  ACTTCCTGGAAGGTGATCAGCGTTGGATCAACACTGTGGCTCAGGCCTTACACCTTCTCAGTACTCTCTA
  TGGGCCCTTTCCTAACTGCTATGGCATTGGCAGATGTGCAAAGATGTCATATGACCTGTGGAGGAAACTG
  GAGGAGGAAGAAGACAGTGAAACCAAAGGTCGGAAACCAGAGATTGGACACATCTTTCTCCTGGACAGAG
  ATGTGGACTTTGTGACAGCACTTTGCTCCCAAGTGGTTTACGAGGGCTTGGTAGATGACACCTTCCGAAT
  CAAGTGTGGAAGTGTTGACTTTGGCCCAGAAGTCACATCCTCTGACAAGAGCCTGAAGGTGCTACTCAAC
  GCTGAGGACAAGGTGTTCAGTGAGATCCGCAATGAGCACTTCTCCAATGTCTTTGGCTTCTTGAGCCAGA
  AGGCCCGGAACTTGCAGGCCCAATATGACCGCCGGAGAGGCATGGACATAAAGCAGATGAAGAACTTCGT
  GTCGCAAGAGCTCAAGGGACTGAAGCAGGAGCACCGCCTTCTGAGTCTCCATATTGGGGCTTGTGAATCA
  ATCATGAAGAAGAAAACCAAGCAGGACTTCCAGGAGCTAATCAAGACCGAGCATGCGCTGCTGGAGGGCT
  TCAACATCCGAGAGAGCACTAGCTACATTGAAGAGCACATAGACCGGCAGGTGTCGCCCATAGAGAGCCT
  ACGCCTCATGTGCCTTTTGTCCATCACTGAGAATGGTTTGATACCCAAGGATTATCGGTCCCTGAAAACA
  CAGTATCTGCAGAGCTATGGCCCCGAGCACCTGCTAACCTTCTCCAACCTGCGGAGAGCCGGGCTTCTAA
  CAGAGCAGGCTCCTGGGGACACCCTCACAGCAGTAGAGAGTAAAGTGAGCAAGCTGGTGACGGACAAGGC
  TGCAGGGAAAATCACTGACGCCTTCAGTTCTCTGGCCAAGAGGAGCAATTTTCGTGCCATCAGCAAAAAG
  CTGAATTTGATCCCACGTGTAGATGGGGAGTATGACCTGAAAGTGCCCCGAGACATGGCTTATGTCTTCA
  GTGGTGCCTATGTGCCTCTGAGCTGCCGAATCATTGAGCAGGTGCTGGACCGGCGGAGTTGGCAGGGCCT
  TGATGAAGTGGTACGGCTGCTAAACTGCAGTGAGTTTGCATTCACAGACACGGCTAAGGAAGACAAGGCT
  TCCAGTGAGTCACTGCGCCTCATCTTGGTGGTGTTCCTGGGGGGCTGCACGTTCTCAGAGATATCAGCCC
  TGCGCTTCCTGGGTAGAGAGAAAGGGTACAGATTTATTTTTCTGACAACTGCTGTTACAAACAGTGCTCG
  CCTCATGGAAGCCATGAGTGAGGTGAAATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178070
• Insert Size: 1854 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178070.4, NP_835171.2
• RefSeq Size: 2595 bp
• RefSeq ORF: 1854 bp
• Locus ID: 233405
• UniProt ID: P59016
• Cytogenetics: 7 D2
• Gene Summary: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating in part VIPAS39 (By similarity). In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependentrecycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (PubMed:25947942).[UniProtKB/Swiss-Prot Function]