Os9 (NM_177614) Mouse Untagged Clone

CAT#: MC219754

Os9 (untagged) - Mouse amplified in osteosarcoma (Os9), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Os9
• Synonyms: 4632413K17Rik; AU022351
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219754 representing NM_177614
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAGGTGCTGCTGTCCAGTCTGTTGGGATTGCTATTTCTAGGGCTCCTGTTACCTGCGCGTC
  TGACGGGCGGTGTCGGGAGCCTGAATTTGGAGGAGCTGAGTGAGATGCGTTATGGCATCCAGATCTTGCC
  GTTGCCTGTCATGGGAGGGCAGAGCCAAGCTTCAGACGTGGTGGTTGTGTCTTCAAAGTACAAACAGCGC
  TATGAGTGCCGCCTACCAGCTGGAGCTATTCACTTCCAACGTGAAAGAGAGGAGGAGACGCCTGCTTACC
  AGGGGCCCGGGATCCCTGAATTGTTGAGCCCAATGAGAGATGCCCCTTGTTTGCTGAAGACCAAGGACTG
  GTGGACATATGAATTCTGTTATGGACGTCATATCCAGCAGTACCATATGGAAGACTCGGAGATCAAAGGT
  GACGTCCTCTATCTTGGCCACTACCAGTCCTCCTTCAACTGGGACGACGAAACAGCCAAGGCTTCCAAGC
  AGCATCGGCTGAAACGCTACCACAGCCAGACCTACGGCAACGGGTCCAAATGTGATCTCAACGGGAAGCC
  CCGAGAAGCTGAAGTTCGGTTCCTGTGTGACGAGGGTGCGGGCATATCTGGGGACTACATTGACCGAGTA
  GATGAACCCGTCTCCTGCTCCTACGTACTGACCATTCGCACGTCAAGGCTCTGCCCGCATCCTCTCCTCC
  GGCCACCAGCCAGCGCTGCCCCACAGGCCATTCTTTGTCACCCAGCCCTGCAGCCTGATGAGTACATGGC
  CTACCTCCAGAGGCAAGCTGAGTCAAAGCAGCATGAAGAGAAAACCACAGAGGAAGTCCAGGACACAGAC
  CGCCAAGTGTGGAGTGGGAGCAAGGCTGCCGGAGCACCCCCAAAGAAAGAAGATGTCAGCCCAGCCAAGG
  AAGAGAAGGAATCAGAGCTCTGGAAGCTTCAGGGGCCAGAGGAGCAGGCAGCAGCAAGAGAGGAGGCGCA
  GGCAGGGGAACAGGACCTGAACCACGAGGCCGCAGCAGATCCAGCTCCAAGCCCTCCCAATGATTTTCAG
  AATAACGTGCAGGTGAAACTCATCCGGAGTCCCGCAGACTTGATTCGACTGATTGAGGAGCTGAAAGCTG
  CAGAAAAGGGGAAGCCAAGCGTAAGGCGGGAGCAGCCTGGAGACGATACCACGGAGGCCCCCCAGAGGGA
  AGCAGAGGGAACGAAGGCGAAGGGAAAGGATGGTGAGCCCCCGGGTCTTATGGAAGAGGAGGATGGTGAC
  GACGAAGAGGAGGAGGAGGAGGAGGAAGAGGACGAGGAGGAGCAGCAGCTCCTGGGAGAGTTCGAGAAGG
  AGCTGGAGGGGATGTTGCTGCCCTCAAACCGCGAGCGCCTCCGCTCTGAGGTTAAGGCTGGCATGGAGCG
  GGAGCTGGAGAACATCATCCAGGAGACAGAGAAGGAACTGGACCCAGAAGGGCTGAGGAAGGAGTCCGAG
  CGGGAGCAGGCAATATTGGCTCTAACATCCACTCTGGACAAACTCATCAAGAGGCTGCAGGAGAACCAGA
  GTCCAGAGCTTGTGCAAAAATACAAAAAAAGGAGAGTTGTCCCCCAAAAGCCTCCCCCATCACCACACCC
  TACAGGCAAGATTGAGATCAAGATTGTGCGACCCGGGGCTGAAGGTAAGGAAGAGGACACACGCTGGCTG
  ACTGATGAGGATACAAGAAACCTTAAGGAGATTTTCTTCAATATCTTGGTACAGGGAGCCGAAGAGGCCA
  ATAAAGAGCGCCAGCGACAGAGTGAGCTGGAGAGCAACTACCGCCGGGTGTGGGGCTCTCCCGGTGGTGA
  AGACACGGGGGACCTGGATGAATTTGACTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177614
• Insert Size: 1854 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177614.3, NP_808282.2
• RefSeq Size: 3884 bp
• RefSeq ORF: 1854 bp
• Locus ID: 216440
• UniProt ID: Q8K2C7
• Cytogenetics: 10 D3
• Gene Summary: Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is a shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.