Rmi1 (NM_028904) Mouse Untagged Clone

CAT#: MC219739

Rmi1 (untagged) - Mouse RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (Rmi1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rmi1
• Synonyms: 4932432N11Rik; C79893
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219739 representing NM_028904
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTAGCTAGTGCTGTATTAAGAGTTGAAACCTGGCTTTTGGCAACATGGCATGTTAAGGTACCTC
  CAATGTGGCTGGAAGCTTGTGTTAACTGGATCCAAGAAGAAAATAATAATGCTACTTTGAGTCAGGCACA
  AATAAATAAACAAGTGTTGGAGCAATGGCTTCTTACTGACCTGAGAGACTTGGAACATCCTCTCTTACCT
  GATGACATTTTAGAACTGCCAAAGGGAGAACTGAATGGGTTTTATGCTCTACAGATCAATTCTTTGGTTG
  ATGTGAGTCAGCCTGCTTATTCACAGATACAGAAGCTGAGAGGAAAGAATACAACCAATGATCTCGTCTC
  AGCTGAAACGCAGAGTACTCCAAAACCATGGGAAGTGAGGCCTTCTCGGATGCTGATGCTACAGCTCACT
  GATGGTGTCACACACATTCAGGGAATGGAGTATCAGTCTATCCCAGCTCTCCATAGTGGTCTTCCTCCAG
  GTACAAAAATTTTAGTTCGTGGATGCATTTTGTTCCGTCTTGGTGTTCTCTTACTGAAACCAGAAAATGT
  GAAGGTGCTAGGGGGTGAGGTAGATGGTCTTTCAGAAGAAAATGCCCAAGAAAAAGTACTTGCAAGATTA
  ATTGGGGAACTTGATCCTACAGTTCCAGTCATTCCAAATAATTCCATTCACAACGTGCCCAAAGTTTCAG
  GGGGCTTAGATGCTGTTTTGGGGCCCTCGGATGAAGAACTCTTGGCAAGTCTTGATGAAAGTGAAGAGTC
  TGCAGCAAATAATGACGTGGCTATGGAAAGAAGCTGTTTCAGCACAGGCACTTCCTCAAATACTACTCCG
  ACAAATCCGTCTGGTTTTGAGCCAGGATGTAACATTTCTTCAAGGCCAAAGGAGAAACCACCAAACCAGC
  CCACGCATTTCACTGATGGAGAATTTGATGACTTTTCACTGGAAGAGGCCTTGCTTTTGGAAGAGACTGT
  CCAGAAAGAACAGATGGAAACAAAAGCATCGCAGCCACTAACTTTGAAGGAAAACACTGGTAAATGTATG
  GAGATTTTTTCACATAAACCTAGTAGTCTGAACCACACAGCTTTGATTCATAAACAAGGAAACAGCAATT
  TTGATGAAAAAACATCTGAACAAATGATTCATGAAGACAAATTTTTTGATTGTGCATCTACTAGAAACCA
  TCATAAGAGATTCTCAGCTCATGATTTTACAAATGACAGTAAGATTTCAGAAGTAGATGATGCAGCACAA
  CAGACCCTCAGCAGTTCAAATGTACATTGCTTACGTAATAAAATATTAAACAGAAAGCTGGACCTATCAG
  AAAAGAGTTCACAAATTTCTAAAGAAAATGGCCACCCTTTCCAGGCTTGTTCTTCAAGATCATTTGAGAA
  TAATACTTATCTATCTATTGGCATGGACTTACATTCTCCACCCTTTATCTATTTGTCTGTTCTAATGGCC
  AGAAAGCCAAAGGAAGTTACTACTGTGACAGTCAAAGCGTTTATTGTCACTTTAACTGGAAATCTCTCAA
  GTTCTGGTGGCTTTTGGGGTGTAACTGCAAAAGTTTCTGATGGTACTGCATATCTAGATGTAGATTTTAT
  AGACGAAATACTTACCAGTATGATAGGGTATTCAGTACCAGAAATGAAACAATTAAGAAAGGACCCTCTT
  AAATATAAAACATTCCTAGAAGGGTTACAGAAATGTCAGCGAGATCTGATCGATTTGTGTTGCCTGATGA
  CTATTTCATACGATCCTTCTTCATGTAAAGGGGTGGTGCTGGAATTGCAAGATGTTGGTATGGAACACGT
  GGAGAACCTAAAGAAACGGTTGAATAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028904
• Insert Size: 1851 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028904.3, NP_083180.3
• RefSeq Size: 3522 bp
• RefSeq ORF: 1851 bp
• Locus ID: 74386
• UniProt ID: Q9D4G9
• Cytogenetics: 13 B1
• Gene Summary: Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.