Dtx2 (NM_023742) Mouse Untagged Clone

CAT#: MC219736

Dtx2 (untagged) - Mouse deltex 2 homolog (Drosophila) (Dtx2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dtx2
• Synonyms: 2610524D08Rik; AA408415; AU022494; Deltex2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219736 representing NM_023742
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGCCCCAAGCTCGTCTCTGCCGCAGGTGTACCCTAGCCACGTGGTGGTGGCTGTGTGGGAAT
  GGCAGGATGGGCTTGGCATTTGGCACCCCTACAGTGCCACTGTCTGCTCCTTCATTGAGCAGCATTTTGT
  CCGGCAGAGGGGCCAGCATTTTGGACTGGGAAGCCTGGCCCACAGCATCCCCTTAGGCCAAGCTGACCCC
  TCACTGGCCCCTTACATCATCGACCTCCCCAGCTGGACTCAGTTTCGCCAGAACACTGGCACCATGAGGT
  CTGTGCGCAGACACCTGTTCTCACAGAATTCTGCCCCAGGCCAGGGCATCGTCTGGGAGTGGCTGGGCGA
  TGACGGATCCTGGGTAGCCTATGAAGCTAGAATCTGTGACTATCTGGAACAGCAAGTGGCCCGGGGCATC
  CAGGTCGTGGACTTGGCACCACTGGGGTATAACTATACTGTCAACTATGCCACCCTAACCCAAACCAACA
  AGACTTCCAGCTTCTGCCGGAGTGTGCGACGCCAAGTGGGGCCAGTTTACCCAGTGACTTCAGACATCGC
  TGTTCCACGCCAAATGGGACTTATCTGCTTTTGCCAACAGTGCCTCCATGGTAGCGGAACTGGCCCTGTG
  TCGGGCCGCTACCGCCACTCCATGACCAACCTGCCTGCATATCCTGCCCCCCAAGCACCCCACCGGACCA
  CCACTGTCTCTGGGGCCCACCAGGCCTTTGCCCCATACAATAAACCTTCACTGTCTGGGGCCAGATCTGC
  ACCAAGGTTGAACACCACCAACCCCTGGGCTGCAGCACCTCCTGTTGCAGGCAACCAGTCCCTGTTCCAC
  TCCAGCCTCTCCCACCTGGGGCCTCAGCTCCTGCCCTCAGGACCGTCCACCTCCAGTGGAGCCAGTGCCT
  CCTTCCCCAGCGGGCCCTCCTCCAGCAGCCCAGGGAGCGCCCCCACCACTGTGCCCGTGCAGATGCCAAA
  GGCCAGCAGGGTCCAGCAGGCGCTTGCAGGCATGACGAGTGTGCTGTCAGCCATTGGACTCCCTGTGTGT
  CTTAGCCGTGCACCCCGGCCCACCGGCCCTCCCGCCTCCCGTCCGGCCTCTAAAAGTCACAGCTCAGTTA
  AGAGGCTGAGGAAAATGTCCGTAAAAGGGGCGCCTAAGCCCGAGCCGGAGCAGGTGATCAGAAAGTACAC
  CGAGGAGCTGAAAGTGGCCCCTGAAGAGGACTGCATTATCTGTATGGAGAAACTGGCTGTGGCTTCTGGG
  TACAGTGACATGACTGACAGCAAGGCCCTCGGGCCCATGGTCGTGGGCCGCCTCACCAAGTGCAGCCATG
  CCTTCCACCTGCTGTGCCTGCTGGCCATGTATTGCAACGGGAACAAGGACGGGAGTCTTCAGTGTCCGTC
  GTGCAAAACCATCTATGGGGAGAAGACTGGGACGCAGCCCTGGGGGAAGATGGAGGTGTTCAGGTTCCAA
  ATGTCCCTCCCAGGCCATGAAGACTGTGGGACTATACTCATTGTTTACAACATCCCTCATGGCATCCAGG
  GACCAGAGCACCCCAGCCCTGGGAAGCCATTCACTGCCCGGGGCTTTCCCCGGCAATGCTACCTGCCAGA
  TAGCCCTCAGGGCCGCAAGGTCCTGGAGCTCCTGAAGGTGGCCTGGAAGAGGCGACTCATTTTTACCGTG
  GGCACTTCCAGCACCACAGGTGAGACCGACACCGTGGTGTGGAACGAGATCCACCACAAGACCGAGATGG
  ACCGCAACGTGACAGGCCACGGCTATCCGGACCCCAACTACCTGCAGAATGTGCTGGCTGAGCTCGCTGC
  CCAGGGAGTGACCGAAGACTGCCTGGAACAGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023742
• Insert Size: 1857 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023742.2, NP_076231.1
• RefSeq Size: 2656 bp
• RefSeq ORF: 1857 bp
• Locus ID: 74198
• UniProt ID: Q8R3P2
• Cytogenetics: 5 G2
• Gene Summary: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1.