Sele (NM_011345) Mouse Untagged Clone

CAT#: MC219711

Sele (untagged) - Mouse selectin, endothelial cell (Sele), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sele
• Synonyms: CD62E; E-selectin; Elam; ELAM-1; LECAM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219711 representing NM_011345
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCAACTGCTGGAGTCATGAATGCCTCGCGCTTTCTCTCTGCTCTTGTTTTTGTTCTCCTCGCTG
  GAGAGAGCACAGCTTGGTACTACAATGCCTCCAGTGAGCTCATGACGTATGATGAAGCCAGTGCATACTG
  TCAGCGGGACTACACACATCTGGTGGCAATTCAGAACAAGGAAGAGATCAACTACCTTAACTCCAATCTG
  AAACATTCACCGAGTTACTACTGGATTGGAATCAGAAAAGTCAATAACGTATGGATCTGGGTGGGGACGG
  GGAAGCCTCTGACAGAGGAAGCTCAGAACTGGGCTCCAGGTGAACCAAACAACAAACAAAGAAATGAGGA
  CTGTGTAGAGATTTACATCCAACGAACCAAAGACTCGGGCATGTGGAATGACGAGAGATGTAACAAAAAG
  AAGCTGGCTCTGTGCTACACAGCTTCGTGTACCAATGCATCCTGCAGTGGTCATGGTGAATGCATAGAGA
  CCATCAATAGTTACACCTGCAAGTGCCACCCTGGCTTCCTGGGACCCAACTGTGAGCAAGCTGTGACTTG
  CAAACCACAGGAACACCCTGACTATGGAAGCCTGAACTGCTCCCACCCGTTCGGCCCCTTCAGCTATAAT
  TCCTCCTGCTCCTTTGGCTGTAAAAGGGGCTACCTGCCCAGCAGCATGGAGACCACCGTGCGGTGTACGT
  CCTCTGGAGAGTGGAGTGCGCCTGCTCCAGCCTGCCATGTGGTTGAATGTGAAGCTTTGACCCACCCTGC
  CCACGGTATCAGGAAATGTTCCTCAAATCCTGGGAGCTACCCATGGAACACGACATGCACGTTTGACTGT
  GTGGAAGGGTACAGGCGAGTTGGAGCTCAGAATCTACAGTGTACCTCATCTGGCATCTGGGATAACGAGA
  CGCCATCATGCAAAGCTGTGACCTGTGACGCCATCCCTCAGCCTCAGAATGGCTTTGTGAGCTGCAGCCA
  CTCAACAGCTGGAGAACTTGCGTTTAAGTCATCCTGTAACTTCACCTGTGAGCAGAGTTTCACGTTGCAG
  GGGCCAGCGCAGGTTGAATGCAGCGCACAAGGGCAGTGGACACCACAAATCCCAGTCTGCAAAGCTGTCC
  AGTGTGAAGCCTTATCTGCGCCACAGCAGGGCAACATGAAATGTCTTCCCAGTGCTTCTGGACCTTTCCA
  AAATGGGTCCAGTTGTGAGTTCTCCTGCGAAGAAGGATTTGAACTGAAGGGATCAAGAAGACTTCAGTGT
  GGTCCAAGAGGGGAATGGGATAGCAAGAAGCCCACGTGTTCAGCTGTGAAATGTGATGATGTCCCTCGGC
  CCCAGAATGGCGTCATGGAGTGTGCTCATGCTACTACTGGAGAATTCACCTACAAGTCCTCATGTGCCTT
  TCAATGCAATGAGGGCTTTAGCTTGCATGGCTCAGCTCAACTTGAGTGCACATCTCAGGGAAAGTGGACC
  CAGGAAGTCCCCTCCTGCCAAGTGGTACAATGTCCAAGCCTTGACGTCCCGGGAAAGATGAACATGAGCT
  GCAGCGGAACAGCAGTTTTCGGCACAGTGTGTGAGTTTACATGTCCTGATGATTGGACACTCAATGGATC
  TGCAGTTCTGACGTGTGGTGCCACGGGACGCTGGTCTGGGATGCCGCCTACCTGTGAAGCCCCAGTCAGC
  CCCACCCGTCCCTTGGTAGTTGCACTTTCTGCGGCAGGAACCTCACTCCTGACATCGTCCTCATTGCTCT
  ACTTGTTGATGAGATACTTTCGGAAGAAAGCAAAGAAATTTGTTCCTGCTAGCAGCTGCCAAAGCCTTCA
  ATCGTTTGAAAACTACCATGTGCCTTCTTACAACGTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011345
• Insert Size: 1860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC119083, AAI19084
• RefSeq Size: 2030 bp
• RefSeq ORF: 1860 bp
• Locus ID: 20339
• UniProt ID: Q00690
• Cytogenetics: 1 71.35 cM
• Gene Summary: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1 (PubMed:1375914). May have a role in capillary morphogenesis.[UniProtKB/Swiss-Prot Function]