9030617O03Rik (NM_145448) Mouse Untagged Clone

CAT#: MC219685

9030617O03Rik (untagged) - Mouse RIKEN cDNA 9030617O03 gene (9030617O03Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 9030617O03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219685 representing NM_145448
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATCTCATTTCTCCTGAGGTCCTGTCTTCGCTCTGCTGTAAGGAGTCTACCCAAGGCAGCACTTA
  TCAGAAACACTTCCAGCATGACGGAAGGACTCCAGCCGGCTAGTGTGGTGGTCCTGCCCAGATCCCTAGC
  ACCAGCTTTTGAAAGCTTCTGCCAGGGCAACCGGGGTCCTCTGCCCCTCCTTGGACAAAGTGAGGCGGTG
  AAGACACTCCCTCAGCTGAGCGCTGTTTCAGACATAAGGACCATCTGTCCACAGTTGCAGAAATACAAGT
  TTGGCACCTGCACAGGCATCCTGACCTCACTGGAAGAGCACTCAGAACAACTAAAAGAAATGGTGACCTT
  CATCATAGACTGCAGCTTCTCCATAGAAGAGGCCTTGGAGCAGGCAGGGATCCCCAGAAGAGACCTAACA
  GGTCCCAGCCATGCAGGAGCATACAAGACAACAGTGCCCTGTGCCACCATTGCTGGCTTCTGCTGCCCTC
  TGGTGGTCACAATGAGACCCATTCCCAAGGACAAGCTGGAAAGGCTGTTGCAGGCCACTCACGCCATAAG
  AGGACAGCAAGGACAACCCATTCACATCGGTGACCCAGGTCTTTTGGGAATTGAGGCACTTTCCAAACCT
  GACTACGGGAGTTATGTGGAGTGTCGGCCCGAGGATGTCCCTGTGTTCTGGCCATCTCCGCTGACCAGTC
  TGGAAGCAGTCATCAGCTGCAAGGCTCCATTGGCTTTCGCCAGCCCTCCAGGCTGCATGGTGATGGTCCC
  GAAGGACACAGCGTCTTCAGCCAGTTGTCTGACTCCTGAGATGGTTCCAGAAGTCCATGCCATTTCCAAA
  GACCCTTTGCATTACAGCATAGTGTCAGCCCCTGCTGCTCAAAAGGTCAGAGAGCTAGAGTCCACAATTG
  CCGTAGACCCAGGGAACCGAGGAATCGGGCACCTACTACTTAAAGATGAGCTACTGCAAGCTGCTTTGTC
  ACTGTCTCATGCCCGCTCCGTACTCGTCACCACTGGATTCCCAACACATTTCAATCATGAGCCCCCAGAA
  GAGACAGATGGCCCACCAGGAGCCATCGCCTTAGCTGCCTTCCTACAGGCTCTGGGGAAGGAGACCGCCA
  TGGTAGTAGACCAGAGAGCCTTGAACTTGCATATGAGGATTGTTGAAGACGCCATTAGGCAAGGAGTTCT
  AAAGACACCGATTCCCATATTAACTTACCAAGGAAGATCCATGGAAGATGCTCGGGCATTTTTGTGCAAA
  GATGGGGACCCTAAGTCTCCTAGATTTGACCATCTGGTGGCCATAGAGCGTGCGGGAAGGGCTGCTGATG
  GCAATTACTACAACGCGAGGAAGATGAACATCAAACACTTAGTTGACCCCATTGATGACATTTTCCTTGC
  TGCACAAAAGATTCCTGGCATCTCATCAACTGGTGTTGGTGACGGAGGCAATGAGCTTGGAATGGGCAAA
  GTAAAGGCGGCCGTGAAGAAGCACATTAGAAATGGAGATGTCATTGCCTGTGATGTGGAGGCTGATTTTG
  CTGTCATTGCCGGTGTTTCTAACTGGGGAGGCTACGCCCTGGCCTGTGCACTGTATATTCTGAACTCATG
  TCAAGTCCATGAGCGCTACCTGAGGAGGGCAACTGGACCTTCCAGGAGAGCTGGGGAACAGAGCTGGATC
  CAGGCCCTGCCATCTGTCGCTAAGGAAGAAAAGATGTTGGGCATCCTGGTAGAGAACCAAGTCCGCAGTG
  GTGTCTCAGGCATCGTGGGCATGGAAGTGGATGGGCTGCCTTTCCATGACGTTCATGCTGAGATGATCCG
  GAAGCTGGTGGGTGCCACCACAGTGCACATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145448
• Insert Size: 1854 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145448.4, NP_663423.2
• RefSeq Size: 3229 bp
• RefSeq ORF: 1854 bp
• Locus ID: 217830
• UniProt ID: Q8BH86
• Cytogenetics: 12 E
• Gene Summary: D-glutamate cyclase that converts D-glutamate to 5-oxo-D-proline.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Both variants 2 and 3 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.