Map3k9 (NM_177395) Mouse Untagged Clone

CAT#: MC219673

Map3k9 (untagged) - Mouse mitogen-activated protein kinase kinase kinase 9 (Map3k9), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map3k9
• Synonyms: E130314H24Rik; Mlk1; Prke1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219673 representing NM_177395
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCTCCAGATCGCTTCTCGGCTGCCTGGCGAGTGCCACTGCCGCCCCGCCGGGGGACGATGCCA
  CGGGCGCTGGGGCCGAAGAGGAGGAGGACGAGGAGGAGGCGGCGGCCGAGCTGGGATCTCACGCCGCGCT
  GCCCTACTGGACGGCTGTGTTCGAGTACGAGGCGGCGGGCGAGGACGAGCTGACCCTGCGGCTGGGCGAT
  GTGGTAGAGGTGCTGTCCAAGGACTCGCAGGTGTCCGGCGATGAGGGCTGGTGGACCGGACAGCTGAACC
  AGCGGGTGGGCATCTTCCCCAGCAACTACGTGACCCCGCGTAGCGCCTTCTCCAGCCGCTGCCAGCCGGG
  CGCCGAGGACCCCAGCTGCTACCCGCCCATTCAGCTGTTAGAGATTGATTTTGCGGAGCTAACCCTGGAG
  GAGATCATCGGCATTGGGGGCTTTGGGAAAGTTTATCGTGCTTTCTGGGCGGGCGATGAGGTGGCCGTGA
  AGGCAGCTCGTCACGACCCTGATGAGGACATCAGCCAGACCATAGAGAACGTTCGCCAAGAGGCCAAGCT
  CTTTGCCATGCTGAAGCACCCGAACATCATTGCGCTCAGAGGGGTGTGCCTGAAGGAACCCAACCTCTGC
  TTGGTCATGGAGTTTGCTCGTGGAGGGCCTCTGAACAGAGTATTGTCTGGGAAGAGGATTCCCCCGGACA
  TCCTGGTGAACTGGGCCGTGCAGATCGCCAGAGGGATGAACTATCTACATGATGAGGCGATCGTACCCAT
  CATCCACCGAGACCTTAAGTCCAGCAACATATTGATCCTGCAGAAAGTGGAGAATGGAGACCTGAGTAAC
  AAGATTCTGAAGATCACCGACTTTGGGCTGGCGCGGGAATGGCACCGGACCACCAAGATGAGTGCGGCGG
  GAACATACGCTTGGATGGCACCTGAAGTCATCCGTGCTTCCATGTTTTCCAAAGGCAGCGATGTGTGGAG
  CTACGGTGTACTGCTTTGGGAGCTGTTGACTGGCGAGGTGCCCTTCCGGGGCATTGATGGCTTAGCAGTG
  GCTTACGGTGTGGCCATGAACAAACTCGCCCTTCCTATCCCCTCTACATGTCCAGAGCCTTTTGCCAAAC
  TCATGGAAGACTGCTGGAATCCCGACCCCCACTCGCGCCCATCTTTCACGAGTATCCTGGACCAGCTAAC
  GACTATAGAGGAGTCCGGTTTCTTTGAGATGCCCAAGGACTCCTTCCACTGCCTGCAGGACGACTGGAAA
  CATGAGATTCAGGAGATGTTTGACCAACTCAGGGCCAAAGAAAAGGAGCTCCGAACCTGGGAAGAGGAGC
  TGACCCGGGCTGCGCTCCAGCAGAAGAACCAGGAGGAGCTGCTCCGGCGTAGGGAGCAGGAGCTGGCGGA
  GCGGGAGATCGACATCCTGGAGCGAGAGCTCAATATCATCATCCACCAGCTGTGCCAGGAAAAGCCCCGG
  GTGAAGAAACGCAAGGGCAAGTTCAGGAAGAGCCGGCTGAAGCTCAAGGACGGCAACCGCATCAGCCTCC
  CCTCCGATTTCCAGCACAAGTTCACGGTGCAGGCCTCCCCGACCATGGATAAAAGGAAGAGTCTGATCAG
  CAACCGGTCGAGTCCTCCTGCAAGCCCCACCATCATCCCTCGCCTTCGAGCCATCCAGTTGACACCTGGT
  GAAAGCAGTAAAACCTGGGGCCGGAGCTCAGTTGTTCCAAAAGAGGAAGGGGAGGAGGAGGAGAAGAGGG
  CCCCAAAGAAGAAGGGCCGGACATGGGGACCAGGAACACTTGGGCAGAAAGAGCTCACATCAGGAGATGA
  AGGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177395
• Insert Size: 1827 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177395.5, NP_796369.2
• RefSeq Size: 3396 bp
• RefSeq ORF: 1827 bp
• Locus ID: 338372
• UniProt ID: Q3U1V8
• Cytogenetics: 12 D1
• Gene Summary: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.