Arhgef25 (NM_001166413) Mouse Untagged Clone

CAT#: MC219625

Arhgef25 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 25 (Arhgef25), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef25
• Synonyms: 2410008H17Rik; D10Ertd610e; GEFT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219625 representing NM_001166413
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGTCGCAAGGATCCATAAATTCAGTTACGAGGGCCATATGCCAAGTTCTGGTTGACGCTTTCA
  GACCCCCGGAGAGAGATCCCTTAGGGCCTGGGAGTACCAAGACAGAGAGTGATTGTATCGAGGAGGATCA
  GACAGGGCAGCGCGAACCTGAGGTCCTCGCCTGGGCTCCGCAGCCCGAATCCTATTCTATTGCCGTCAGT
  GAGGGGAGCATGTCAGCGTCTGCTGTCTCGGGGCTGGCTGCCCTGTCTGGCCCCAGCTCTGGCCTCAGCT
  CTGATCCGTGTTCTCCGATTCCCCCTGGACCAGTAACTGGCCTGAGGAGATGGTTGGATCATTCCAAACA
  TTGTCTCAGTGTGGAAACTGAGGCAGAGAGTGGTCAGACCGGACAATGTGAGAACTGGACGCTGGAGCCA
  ACTTTAACCACAGGACAAGAGCTGCCCGAACTGACCTTACTGACTACATTGCTGGAGGGTCCTGGAGACA
  AGGCACAGCCAGCTGAAGAGGAAACTTTGTCCCAAGCTCCCAAGAATGAGGAAGAGCAGAAGAAGATGGC
  TCTGGAAAGGAGTATGTTTGTCCTGGGTGAGCTGGTGGAAACAGAAAGAACATATGTGGACGACTTGGGA
  CAGATCGTAGAGGGTTACATGGCTACCATGGCTACTCAGGGGGTACCTGAGAGTCTCCGAGGTCGTGACA
  GGATTGTGTTTGGGAACATTCAGCAGATCTATGAGTGGCACCGAGACTACTTCCTGCAGGAGCTGCAGCA
  GTGTTTGAAAGATCCCGATTGGTTGGCTCAGCTGTTCATCAAACATGAGCGCCGACTGCATATGTATGTG
  GTGTACTGCCAGAATAAACCCAAGTCAGAGCATGTGCTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGC
  TGAGGCAGCAGCTGGGACACCGACTGCAGCTCAATGACCTCCTCATTAAACCAGTGCAGCGAATCATGAA
  ATACCAGTTGCTGCTGAAGGATTTTCTAAAGTATTACAGAAGAGCTGGGAAGGATACTGAAGAGCTGGAG
  CAAGCTGTGGAGGTCATGTGTTTCGTACCTAAGCGATGCAATGATATGATGTCCCTGGGGAGACTTCGGG
  GATTTGAGGGAAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACATTCTTGGTTACAGAGCCTGA
  AGCTGGGGGTCTGCTTTCTTCCCGGGGTCGGGAGAGGCGTGTCTTCCTGTTCGAGCAAATCATCATCTTC
  AGTGAGGCCCTGGGAGGAGGAGGCCGAGGTGGCGCGCAGCCTGGCTACGTGTACAAGAACAGCATCAAGG
  TGAGCTGCCTAGGGCTGGAGGGGAATCTCCAAGGCAACCCTTGCCGGTTTGCGCTGACCTCTAGAGGGCC
  AGAAGGTGGGATCCAGCGCTACGTTCTGCAGGCTTCAGACCCTGCTGTCAGTCAAGCCTGGATAAAGCAA
  GTGGCCCAGATCCTGGAAAGCCAGCGTGACTTTCTCAACGCATTACAGTCACCCATTGAATACCAGAGAC
  GTGAGAGCCAGACCAATAGCCTGGGGCGACCAGGAGGGCCTTGGGTAGGGAGCCCTGGGAGAATGCGGCC
  TGGAGACCTGGCCCAGGCCAGCATGCACACACCCATCAATGGCTCTCTCCCTTCCCTGCTACTTCTGCCC
  AGAGGGGAGGTGTCCAGAGTGCTGTTGCCCCTGGATACACAGGCTCTCAGTGATACCCCCCAGACTCCTC
  ATGACTCTCCAGCCCTTCCCACTGTGAACACTCCTCCCTGTCAGGCCAGGCTCGCCAAGCTGGATGAAGA
  TGAGCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166413
• Insert Size: 1830 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166413.1, NP_001159885.1
• RefSeq Size: 2199 bp
• RefSeq ORF: 1830 bp
• Locus ID: 52666
• UniProt ID: Q9CWR0
• Cytogenetics: 10 74.5 cM
• Gene Summary: May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation (By similarity). May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.