Ntn1 (NM_008744) Mouse Untagged Clone

CAT#: MC219613

Ntn1 (untagged) - Mouse netrin 1 (Ntn1), (10ug)


  "NM_008744" in other vectors (4)

Reconstitution Protocol

USD 844.00

In Stock*

Size
    • 10 ug

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Frequently bought together (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Ntn1
Synonyms AI561871; BC019633; Netrin-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219613 representing NM_008744
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCGCGCTGTGTGGGAGGCGCTGGCGGCGCTGGCGGCGGTGGCGTGCCTGGTGGGCGCGGTGCGCG
GCGGGCCCGGGCTTAGCATGTTCGCCGGCCAGGCGGCGCAGCCTGATCCTTGCTCGGATGAGAATGGACA
CCCGCGCCGCTGCATCCCGGACTTTGTCAACGCGGCCTTCGGCAAGGACGTGCGCGTGTCCAGCACCTGC
GGCCGGCCCCCGGCGCGCTACTGCGTGGTGAGCGAGCGTGGTGAAGAGCGGCTGCGCTCCTGTCACCTCT
GCAACTCTTCGGATCCCAAGAAAGCGCACCCGCCCGCCTTCCTCACCGACCTCAATAACCCGCACAACCT
GACGTGCTGGCAGTCCGAGAACTACCTGCAGTTCCCGCACAACGTGACGCTCACTCTGTCGCTCGGCAAG
AAGTTTGAGGTGACCTATGTGAGCCTGCAATTCTGCTCGCCGCGGCCAGAGTCCATGGCCATCTACAAGT
CCATGGACTACGGGCGCACGTGGGTGCCCTTCCAGTTCTATTCCACGCAGTGCCGCAAAATGTACAACCG
GCCGCACCGCGCGCCTATCACCAAACAGAACGAGCAGGAGGCCGTGTGCACCGACTCGCACACCGACATG
CGCCCGCTCTCTGGCGGGCTGATCGCTTTCAGCACGCTGGACGGGCGGCCCTCGGCGCACGACTTCGACA
ACTCGCCGGTGCTGCAGGACTGGGTCACGGCCACCGACATCCGCGTGGCTTTCAGCCGCCTGCACACGTT
CGGCGACGAGAACGAAGACGACTCGGAGCTGGCGCGCGACTCCTATTACTATGCAGTGTCTGACCTGCAG
GTTGGCGGCCGCTGCAAGTGCAACGGCCACGCGGCGCGTTGCGTGCGCGACCGAGACGACAGTCTGGTGT
GTGACTGTAGGCACAACACGGCCGGCCCTGAATGCGACCGTTGCAAGCCCTTCCACTACGACCGGCCCTG
GCAGCGCGCCACGGCCCGCGAGGCCAACGAGTGCGTGGCCTGCAACTGCAACCTCCATGCTCGGCGCTGC
AGATTCAACATGGAGCTCTATAAGCTATCAGGGCGCAAGAGCGGGGGAGTCTGTCTCAACTGCCGCCACA
ACACTGCGGGCCGCCACTGCCACTACTGCAAGGAGGGCTTCTACCGAGACATGGGCAAGCCTATCACCCA
CCGGAAGGCTTGCAAAGCCTGTGATTGCCACCCAGTGGGTGCTGCTGGCAAGACCTGCAATCAAACCACT
GGCCAATGTCCCTGCAAGGACGGCGTGACGGGCATCACCTGCAACCGATGTGCCAAAGGCTACCAGCAGA
GCCGCTCCCCCATCGCCCCTTGCATCAAGATTCCTGTGGCGCCACCCACCACTGCAGCCAGCAGCGTGGA
GGAACCGGAAGACTGTGACTCCTATTGCAAGGCCTCCAAAGGCAAGCTGAAGATGAACATGAAGAAATAC
TGCAGGAAGGACTATGCTGTCCAGATCCACATCCTGAAGGCCGACAAAGCAGGGGACTGGTGGAAGTTCA
CCGTGAACATCATCTCCGTGTACAAGCAGGGCACAAGTCGTATTCGCCGTGGTGACCAGAGTTTGTGGAT
CCGCTCACGAGACATCGCCTGCAAGTGTCCCAAAATCAAGCCCCTCAAGAAGTACTTGCTGTTGGGTAAT
GCCGAGGACTCACCTGACCAGAGTGGCATCGTGGCAGACAAGAGCAGCCTGGTGATCCAGTGGCGGGACA
CATGGGCACGGCGGCTGCGCAAGTTCCAGCAACGGGAGAAGAAGGGCAAGTGCAAGAAGGCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_008744
Insert Size 1815 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008744.2, NP_032770.2
RefSeq Size 5757 bp
RefSeq ORF 1815 bp
Locus ID 18208
UniProt ID O09118
Cytogenetics 11 B3
Gene Summary Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]

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