Epha7 (NM_001122889) Mouse Untagged Clone

CAT#: MC219607

Epha7 (untagged) - Mouse Eph receptor A7 (Epha7), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Epha7
• Synonyms: Cek11; Ebk; Ehk3; Hek11; Mdk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219607 representing NM_001122889
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGTTCAAACTCGGTTCCCTTCGTGGATTATTTTGTGTTACATCTGGCTGCTTGGCTTTGCACACA
  CGGGGGAGGCGCAGGCTGCGAAGGAAGTACTATTACTGGACTCGAAAGCACAACAAACAGAATTGGAATG
  GATTTCCTCTCCACCCAGTGGGTGGGAAGAAATTAGTGGTTTGGATGAGAACTACACTCCGATAAGAACA
  TACCAGGTGTGCCAGGTCATGGAGCCCAACCAGAACAACTGGCTGCGGACTAACTGGATTTCTAAAGGCA
  ACGCACAAAGGATTTTTGTAGAATTGAAATTCACCTTGAGGGATTGTAATAGTCTTCCCGGAGTCCTGGG
  AACTTGCAAGGAAACGTTTAATTTGTACTATTATGAAACAGACTACGACACCGGCAGGAATATACGAGAA
  AACCTTTATGTTAAAATAGACACCATTGCTGCAGATGAAAGTTTCACACAAGGTGACCTTGGTGAAAGAA
  AGATGAAGCTGAACACTGAGGTGAGAGAGATTGGACCTTTGTCCAAAAAGGGATTCTATCTTGCCTTTCA
  GGATGTAGGGGCTTGCATAGCATTGGTTTCTGTCAAAGTGTACTACAAGAAGTGCTGGTCCATTGTTGAG
  AACTTAGCTGTCTTTCCAGATACAGTGACTGGTTCGGAATTTTCCTCCTTAGTCGAGGTCCGTGGGACAT
  GTGTCAGCAGTGCCGAGGAAGAGGCAGAAAATTCCCCCAGAATGCATTGCAGTGCAGAAGGAGAGTGGCT
  AGTACCCATTGGAAAATGCATCTGCAAAGCAGGCTATCAGCAAAAAGGGGACACTTGCGAACCCTGTGGC
  CGCAGGTTCTACAAATCTTCCTCTCAGGATCTCCAGTGTTCTCGTTGTCCAACCCACAGCTTCTCTGACC
  GAGAAGGATCATCCAGGTGTGAATGTGAAGATGGGTACTACAGAGCTCCTTCTGATCCACCATACGTTGC
  ATGCACGAGGCCTCCCTCTGCACCACAGAACCTTATTTTCAATATCAATCAAACGACTGTAAGTTTGGAA
  TGGAGTCCTCCGGCTGACAACGGGGGAAGAAACGATGTCACCTACAGAATACTGTGTAAGCGGTGCAGTT
  GGGAACAGGGAGAATGTGTGCCATGCGGAAGTAACATTGGATACATGCCCCAGCAGACGGGATTAGAGGA
  TAACTATGTCACTGTCATGGACCTACTTGCCCATGCAAATTACACTTTCGAAGTTGAAGCTGTAAATGGA
  GTTTCGGACTTAAGCAGATCCCAGAGGCTCTTCGCTGCTGTTAGCATCACCACCGGTCAAGCAGCTCCCT
  CGCAAGTGAGTGGAGTCATGAAGGAGCGAGTACTGCAGCGGAGTGTGCAGCTTTCCTGGCAGGAGCCGGA
  GCATCCCAATGGAGTCATCACGGAATATGAAATCAAGTATTATGAGAAAGATCAACGGGAAAGGACGTAC
  TCAACACTCAAAACCAAGTCCACCTCCGCCTCCATTAATAATCTGAAACCGGGAACAGTGTACGTCTTTC
  AGATCCGGGCGGTCACTGCTGCCGGTTATGGAAACTACAGCCCTAGGCTTGATGTTGCCACACTTGAGGA
  AGCTTCAGGTAAAATGTTTGAAGCGACAGCAGTCTCCAGTGAACAGAATCCTGTCATCATAATTGCTGTA
  GTGGCTGTAGCAGGGACCATCATCTTGGTGTTCATGGTGTTCGGCTTCATCATTGGAAGAAGGCACTGTG
  GTTATAGCAAGGCTGACCAAGAAGGGGATGAAGAACTCTACTTTCATTCTTTAGTAACAAATGAGCACCT
  GTCAGTTTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001122889
• Insert Size: 1833 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122889.1, NP_001116361.1
• RefSeq Size: 3272 bp
• RefSeq ORF: 1833 bp
• Locus ID: 13841
• Cytogenetics: 4 12.42 cM
• Gene Summary: Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7. Isoform 4 which lacks the kinase domain may regulate isoform 1 adhesive properties.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks multiple 3' exons but has an alternate 3' exon, as compared to variant 1. The resulting isoform (2) has a different and shorter C-terminus, as compared to isoform 1.