Mindy4 (NM_177883) Mouse Untagged Clone

CAT#: MC219589

Fam188b (untagged) - Mouse family with sequence similarity 188, member B (Fam188b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mindy4
• Synonyms: C330043M08Rik; Fam188b; Gm1406
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219589 representing NM_177883
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGTCTGTACGTGGAGGAGGTGGCCGCCTCCCTGGTCAGGGAGTTTCTTAGCAGAAAGGGTTTGA
  ACAAGACATTTGTGACCATGGACCAGGAGCGCCCCCGCTGTGAGCTTAGCATCAACAGCAGAAATGATCT
  CCGCAAAGTTCTGCATCTGGAGTTTCTCTACAAGGAGAACAAGGCAAAAGAAAAGCCTCTAAGAACAAAC
  CTGGAGCTCATTACCAGGTACTTTCTGGATAATGTTGGAAATACAGATAACAGTGAAAGTCAAGAAGTCC
  CAATCCCTGCAATCCCTGTTCCAAAGAAAAACAACAAGTTGCCTTTGAGACACTCAGAGACCACATTGGT
  GAATATATACGACCTTTCAGATGAAGATACGGGAAGACGGACATCATGGTCAGAAGCAGGCAAAGCCAGG
  CATGACAGTCTGGATGGGGACATACTTGGTAATTTCGTATCATCCAAAAAACCCTCACACAAAAGTAAGG
  CTGCACACGTGGACCTGGGTGACAGCCTCCCGTTGGTTCCTGCGTGGGAGAAGGTGGACCAGCTTCACTC
  ATCAGAGCCCGGTATAGATGTGAAGAAGACAATGGAGAGGACCAGGCCTAAGTCTGGCCTGATTGTCCGA
  GGCATGATGGCTGGGCCTGTCGCCAGCTCCCCTCAGGACTCTTTCCGCAAACGCTCTCTGAGAAGGTCCT
  CAGCTCTGAGCAGGAAGCTCCAAACCCCAGAGGAGATCCAGCAGCAGTCTGAGCCTTTTGTCCACACCCC
  AGCCTATCTGGGTCCACAGGAGGTCCCCGATTCCAGCAGTGACTCCGTCTCCAGAAGCCCTCTGGGCCAG
  CTGAATGAACTGTCTATAGAGAAGCCAAATGTCACTTCTAGCAGCCAAGGGCTGTCCCAGAGGGACAGAC
  CCAGGCTAAGAAGTGTTTCAGAGGACAGCCCACTGGGGTACAGCCACACAGAGGGGAATTCCAGGATGGC
  CCAGGACCAGCTGGAAAGAGCGTTCAAGCGTCAGGGCGTCCAGCCTCCATCTCTCAGGAAAAATCAGTTG
  GTGTCTGACAGGACGGATGATAAGCCAGATGCCCTGCAGCTAGAGGATGTTGAGGATGAGTTGATAAAGG
  AAGACATTGTCTTGTTTCCACCTCCATCAATGCTCAAGCTTCAGACTGTGTCAAAGCCAATTGACCTCTC
  ACTGGCTAAGGAGATAAAGACCCTTCTGTTTGGCTCTACCTTTTGCTGCTTCAGTGAAGAATGGAAACTT
  CAGAACTTTTCCTTCAATGACATTGCCTCGTTAAAATACGGCATTGTGCAGAACAAGGGTGGTCCCTGTG
  GGGTTCTGGCAGCTGTTCAAGGCTGTGTGCTTCAGAAACTCCTGTTTGAAGGAGATAACAGAACCAACTC
  TAATCTCCGGCTGCAGCCCTCAGATGCCCAGAGGACTCGCTGCCTTGCCCTGGCCATTGCCGACATCCTG
  TGGCGAGCTGGAGGCAAAGAACAAGCCGTGGTTGCACTGGCTTCAGGAACACCGCACTTCAGTCCAACAG
  GGAAATACAAAGCAGATGGAGTCTTAGAAACAGTACGGCTTTCTGGAAAACTATCGTGCCACCTTTGGCT
  TTGTTTGGGGACTATCTCCTTTCATATAAGTAAATCCTACCCCAGCTGGGGCTGGCTGGCCATCCCTGCA
  GCCCAGGCCCCCAGAATCCTGCCTGCCCTTTGGGGGTTGGGGAGAGCTGAGTGGCTTGGTGGTTTGGCGT
  GGTTTGGCATGGTTTGGCGTGGTTTGGCTCATCAGAGCTGGTTCAGTGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177883
• Insert Size: 1803 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177883.4, NP_808551.2
• RefSeq Size: 2211 bp
• RefSeq ORF: 1803 bp
• Locus ID: 330323
• UniProt ID: Q3UQI9
• Cytogenetics: 6 B3
• Gene Summary: Probable hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks multiple 3' exons but has an alternate 3' sequence, as compared to variant 1. The resulting isoform (2) has a shorter and different C-terminus, as compared to isoform 1.