Cdc14a (NM_001080818) Mouse Untagged Clone

CAT#: MC219573

Cdc14a (untagged) - Mouse CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdc14a
• Synonyms: A830059A17Rik; Cdc14; CDC14a1; CDC14A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219573 representing NM_001080818
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGAGTCTGGGGAACTAATCGGGGCTTGCGAGTTCATGAAAGATCGATTATATTTTGCTACTT
  TAAGGAATAGACCAAAAAGCACAATAAATATCCACTATTTCTCCATCGACGAGGAGCTGGTCTATGAAAA
  TTTCTATGCAGATTTTGGACCTCTGAACTTGGCAATGGTGTACAGATACTGCTGTAAGCTAAACAAGAAA
  CTAAAATCATACAGTTTATCAAGAAAGAAGATAGTGCACTACACCTCTTTCGACCAGCGGAAAAGAGCAA
  ACGCAGCATTCTTGATAGGTGCTTATGCCGTCATCTACTTAAAGAAGACACCAGAAGAAGCGTACAGAGC
  TCTCCTGTCTGGCTCAAACCCTCCTTATCTTCCATTCAGGGATGCATCTTTTGGAAACTGCACTTACAAC
  CTCACCGTCCTTGACTGTTTACAAGGAATCAGAAAGGGATTACAGCATGGGTTTTTTGACTTTGAGACGT
  TTGATGCGGAAGAATATGAACATTATGAGCGAGTTGAAAACGGAGACTTCAACTGGATCGTTCCAGGAAA
  GTTTTTAGCGTTCAGTGGACCGCATCCTAAAAGCAAGATTGAGAATGGTTACCCTCTCCACGCTCCCGAA
  GCCTACTTTCCATACTTCAAAAAGAACAACGTGACCACGATCGTGAGATTGAACAAAAAGATCTACGAGG
  CGAAGCGCTTCACAGACGCTGGCTTCGAGCACTATGACCTGTTCTTCATAGACGGCAGCACCCCCAGCGA
  TAACATCGTGCGGAGATTCCTGAACATCTGTGAGAACACCGAGGGGGCCATCGCGGTCCACTGCAAAGCT
  GGTCTGGGAAGAACCGGGACATTGATAGCCTGTTATGTCATGAAACACTACAGGTTTACACATGCTGAAA
  TCATTGCTTGGATCAGAATTTGCCGACCAGGCTCCATCATCGGACCCCAGCAGCACTTCCTGAAAGAAAA
  ACAAGCATCATTGTGGGTCCAAGGAGACATTTTTAGATCCAAACTAAAAAATAGACCATCCAGTGAGGGA
  AGTATTACAAAAATTATTTCTACCCTGGATGATATGTCTATTGGTGCAAATTTATCCAAATTACAAAGCA
  CAGAAAGGATTGGAGAGAATAATTTTGAAGACGAAGATATGGAAATTAAGAACAACGTAACGCAGGGAGA
  CAAACTACGTGCCTTAAAAAGCCAGAGGCATCCCCGCTCCTCGCCATCCTGTGCATTTAGGTCAGATGAT
  ATGAAAGGACACCAAAGGGCAATGGCCCAGACTTTCAGATTAAGTTCCTCACCGCAACCAACTATGTCGA
  CCATGAAGACCTCCAAAGTGTGTTTGTCCCCTTCAGTGACAGCCAAAAAAATAAGCCGAGGTTCTTTGTC
  TTCAGGAGCAAATATAAGAAGCTTCTCCATAAATTCCCGGCTAGCCAGTTCTCTAGGGAACTTGAACGCC
  GGGACAGAAGAACCTGAGACCAAGAAGACCACATCACTCACCAAGGCCGCTTTCATAGCCAGCCCATTCA
  CCAGCTTCTTGAATGGCAGCACCCAGACACCTGGCAGAAACTACCCTGAGCTCAACAACAACCAGTACAC
  CAGAAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCGGTCTTGGGGGCAACCTGAACAGCTCTCCAGTC
  CCCCAGAGCGCCAAGCCCGAGGAGCACACCACCATCCTTCGACCTTCCTTCCCGGGGTCACTCTCTTCCT
  CCTCAGTGAGATTCCTGAGCCGTTCTATACCTTCCCTTCAGTCTGAATACGTTCATTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080818
• Insert Size: 1812 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080818.2, NP_001074287.1
• RefSeq Size: 4511 bp
• RefSeq ORF: 1812 bp
• Locus ID: 229776
• UniProt ID: Q6GQT0
• Cytogenetics: 3 G1
• Gene Summary: Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. Dephosphorylates SIRT2 around early anaphase. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC-FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis (By similarity). Required for normal hearing (PubMed:29293958).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).