Ythdf3 (NM_172677) Mouse Untagged Clone

CAT#: MC219572

Ythdf3 (untagged) - Mouse YTH domain family 3 (Ythdf3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ythdf3
• Synonyms: 9130022A11Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219572 representing NM_172677
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCCACTAGCGTGGATCAGAGACCTAAAGGGCAAGGAAATAAAGTTTCAGTACAAAATGGTTCGA
  TTCATCAAAAAGATGCTGTAAATGATGATGATTTTGAGCCATACTTAAGTAGCCAGACAAATCAGAAATA
  TAGGAGAGCAAAACAGTTGTTTCATTGTAATAACAGCTATCCACCAATGTCAGATCCATATATGCCTAGT
  TACTATGCTCCGTCCATTGGATTTCCATATTCTCTTGGGGAAGCAGCATGGTCCACGGCTGGAGATCAGC
  CTATGCCATATCTGACAACCTATGGACAAATGAGTAATGGAGAACATCATTATATACCAGATGGTGTTTT
  TAGTCAACCAGGGGCATTAGGAAATACCCCTCCATTTCTTGGTCAACATGGATTTAACTTTTTTCCTGGT
  AATGCTGATTTCTCTACATGGGGAACAAGTGGATCTCAGGGACAATCAACACAAAATTCTGCTTATAGTA
  GCAGTTACGGCTATCCACCTAGTTCTCTTGGGAGAGCTATTACTGATGGACAGGCCGGATTTGGCAATGA
  TACTTTGAGTAAGGTGCCTGGCATTAGTAGTATTGAGCAAGGCATGACTGGACTGAAAATTGGTGGTGAC
  CTGACAGCTGCGGTGACGAAAACTGTAGGTACAGCCTTGAGCAGCAGTGGTATGACTAGCATTGCAACCA
  ATAATGTGCCCCCTGTTAGCAGTGCAGCACCTAAACCAACTTCTTGGGCTGCTATTGCCAGAAAGCCTGC
  AAAACCTCAGCCGAAACTTAAACCCAAGGGCAATGTGGGAATTGGGGGTTCTGCTGTGCCACCACCTCCT
  ATAAAACACAACATGAATATTGGAACTTGGGATGAAAAGGGGTCAGTGGTAAAGGCTCCACCAACCCAAC
  CAGTTCTGCCTCCTCAAACTATAATCCAGCAGCCTCAGCCATTAATTCAACCACCACCATTGGTGCAAAG
  CCAACTGCCTCAACAGCAGCCTCAGCCACCACAACCACAGCAGCAACAAGGACCTCAGCCACAGGCCCAG
  CCTCACCAAGTGCAGTCTCAACAGCCACAGTTGCAGAATCGTTGGGTAGCTCCTCGGAATAGGGGAACCG
  GCTTCAACCAGAACAATGGAACAGGCAGTGAAAACTTTGGTTTAGGTGTTGTGCCTGTTAGTGCTTCACC
  TTCTAGTGTAGAGGTGCATCCAGTGCTGGAAAAACTAAAGGCCATAAACAATTATAATCCCAAAGACTTT
  GATTGGAACCTGAAGAATGGACGTGTGTTTATAATTAAGAGCTATTCTGAGGATGATATACATCGTTCCA
  TCAAGTACTCTATCTGGTGTAGTACTGAGCATGGTAATAAGCGTTTGGATGCAGCTTATCGTTCCCTGAA
  TGGGAAAGGCCCACTCTATTTACTCTTCAGTGTGAATGGCAGTGGACATTTTTGTGGAGTGGCTGAAATG
  AAGTCTGTTGTAGACTATAATGCTTATGCTGGTGTCTGGTCTCAGGATAAGTGGAAGGGCAAATTTGAAG
  TTAAATGGATCTTTGTCAAAGATGTTCCCAATAACCAATTACGACATATTCGCTTAGAAAATAATGACAA
  CAAACCAGTAACCAATTCAAGGGACACTCAAGAAGTACCCCTAGAAAAAGCAAAGCAAGTGCTTAAAATA
  ATCGCTACTTTCAAGCATACCACCTCAATCTTTGATGACTTTGCACATTATGAAAAGCGTCAAGAGGAGG
  AGGAAGCCATGCGAAGGGAGAGAAATAGAAACAAGCAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172677
• Insert Size: 1791 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172677.3, NP_766265.3
• RefSeq Size: 5190 bp
• RefSeq ORF: 1791 bp
• Locus ID: 229096
• UniProt ID: Q8BYK6
• Cytogenetics: 3 A1
• Gene Summary: Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs and promotes RNA translation efficiency (By similarity). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (By similarity). Shares m6A-containing mRNAs targets with YTHDF1 and YTHDF2, and regulates different processes depending on the context (By similarity). Facilitates the translation of targeted mRNAs in cooperation with YTHDF1 by binding to m6A-containing mRNAs and interacting with 40S and 60S ribosome subunits (By similarity). Acts as a negative regulator of type I interferon response by down-regulating interferon-stimulated genes (ISGs) expression: acts by binding to FOXO3 mRNAs and promoting their translation (PubMed:30591559). Binds to FOXO3 mRNAs independently of METTL3-mediated m6A modification (PubMed:30591559). Can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation (By similarity). Recognizes and binds m6A-containing circular RNAs (circRNAs) and promotes their translation (By similarity). circRNAs are generated through back-splicing of pre-mRNAs, a non-canonical splicing process promoted by dsRNA structures across circularizing exons (By similarity).[UniProtKB/Swiss-Prot Function]