Klhl24 (NM_029436) Mouse Untagged Clone

CAT#: MC219544

Klhl24 (untagged) - Mouse kelch-like 24 (Drosophila) (Klhl24), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhl24
• Synonyms: 1110046J11Rik; 4930429H24Rik; 6530402O20Rik; C85082
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219544 representing NM_029436
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTACTAATATTGGGACGCAGATTAAACAGAGAGGATCTTGGGGTGCGTGATTCCCCAGCAACTAAGC
  GTAAAGTTTTTGAAATGGACCCCAAATCTCTGACAGGTCATGAGTATTTTGACTTCTCTTCAGGATCATC
  CCATGCTGAAAACATCCTCCAGATATTTAATGAGTTCCGAGACAGCCGCTTATTCACAGATGTCATTATC
  TGTGTGGAAGGAAAAGAATTCCCATGCCATCGAGCTGTCCTCTCAGCCTGCAGCAGCTACTTCAGAGCCA
  TGTTCTGTAACGACCACAGGGAGAGCCGAGAAATGCTGGTTGAGATCAATGGCATTTTAGCTGAAGCTAT
  GGAATGCTTTCTGCAGTATGTGTATACTGGGAAGGTGAAGATCACAACAGAGAATGTCCAGTACCTCTTC
  GAAACCTCCAGCCTCTTTCAGATTAGTGTTCTCCGTGATGCTTGTGCCAAGTTCTTAGAGGAACAGCTTG
  ATCCTTGCAATTGCCTAGGAATCCAGCGCTTTGCCGACACCCACTCGCTCAAAACACTCTTCACAAAATG
  CAAGACCTTTGCATTACAGACTTTTGAGGATGTGTCCCAGCATGAAGAATTTCTCGAGCTTGACAAAGAT
  GAACTTATTGATTATATCTGCAGTGATGAACTTGTTATTGGTAAAGAGGAGATGGTTTTTGAGGCCGTCA
  TGCGCTGGGTCTACCGGGCGGTTGATCTGCGAAGACCACTGTTACATGAGCTCCTGACGCACGTCAGGCT
  TCCTCTCTTGCATCCCAACTACTTTGTTCAGACAGTTGAAGTGGACCAGTTGATCCAGAATTCCCCCGAG
  TGTTATCAGTTGCTGCACGAAGCAAGGCGGTACCACATACTTGGCAATGAAATGATGTCCCCAAGGACTA
  GGCCACGCAGGTCTACTGGCTATTCAGAGGTGATAGTTGTGGTTGGAGGCTGTGAACGAGTTGGAGGATT
  TAATCTTCCATACACTGAGTGCTATGATCCAGTAACAGGAGAATGGAAGTCCTTGGCCAAGCTTCCAGAA
  TTTACCAAATCCGAGTATGCAGTCTGTGCACTGAGGAATGATATTCTTGTTTCAGGTGGAAGAATCAACA
  GCCGTGATGTCTGGATTTATAATTCACAGTTAAATATCTGGATCAGAGTTGCTTCTCTAAATAAAGGCAG
  ATGGCGTCACAAAATGGCTGTCCTTCTTGGTAAAGTGTATGTTGTTGGCGGCTATGATGGGCAAAACAGG
  CTTAGCAGTGTGGAGTGTTACGATTCCTTTTCAAATCGTTGGACGGAAGTTGCTCCACTTAAGGAAGCAG
  TGAGCTCTCCTGCAGTGACAAGCTGTATAGGAAAATTGTTTGTGATTGGTGGAGGACCTGATGATAATAC
  TTGTTCTGATAAGGTCCAATCTTATGACCCAGAAACCAATTCTTGGCTACTTCGTGCAGCTATCCCTATC
  GCCAAGAGGTGCATCACAGCTGTCTCTTTAAACAACCTGATCTACGTAGCTGGTGGGCTGACCAAGGCAG
  TGTATTGTTACGATCCCGTTGAAGACTACTGGATGCATGTACAAAACACATTCAGCCGGCAGGAAAACTG
  TGGTATGTCTGTTTGTAATGGTAAAATCTATATCCTGGGTGGAAGACGGGAAAATGGAGAAGCCACAGAT
  ACTATTCTCTGTTACGATCCTGCGACAAGTATCATCACAGGAGTGGCTGCAATGCCCAGGCCAGTGTCCT
  ACCACGGCTGTGTAACTATTCATAGATACAATGAGAAGTGCTTTAAGCTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029436
• Insert Size: 1803 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029436.3, NP_083712.4
• RefSeq Size: 6683 bp
• RefSeq ORF: 1803 bp
• Locus ID: 75785
• UniProt ID: Q8BRG6
• Cytogenetics: 16 A3
• Gene Summary: Controls KRT14 levels during keratinocytes differentiation (PubMed:27798626). As part of the BCR(KLHL24) E3 ubiquitin ligase complex, mediates ubiquitination of KRT14 (By similarity). Specifically reduces kainate receptor-mediated currents in hippocampal neurons, most probably by modulating channel properties (By similarity).[UniProtKB/Swiss-Prot Function]