Prdm5 (NM_027547) Mouse Untagged Clone

CAT#: MC219535

Prdm5 (untagged) - Mouse PR domain containing 5 (Prdm5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prdm5
• Synonyms: 4432417F03Rik; 6530401I24Rik; AI197291; E130112L17Rik; PFM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219535 representing NM_027547
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCATGTACGTACCAGACAGGTTCGCCCTGAAGTCGTCCCGGGTCCAGGACGGGATGGGGCTCT
  ACACGGCCCGCCGCGTGCGCAAGGGTGAAAAATTTGGACCCTTCGCTGGGGAGAAGCGAATGCCTGAAGA
  CTTGGATGAAAATATGGACTACAGACTGATGTGGGAGGTACGTGGGAGCAAGGGAGAAGTTCTGTATATT
  TTGGATGCTACCAACCCAAGACACTCCAACTGGCTTCGCTTTGTTCACGAGGCACCATCTCAGGAGCGGA
  AGAACCTGGCTGCCATTCAAGAAGGAGAAAATATTTTCTACTTGGCAGTTGATGATATAGAAACAGATAC
  AGAGCTTTTGATTGGCTACCTGGACAGTGATGTGGAGGCAGAGGAGGAGGAGCAACAAGCTCTGACCATG
  ACCAAAGAAGGCAAAGTTGACCACTCTAAGGGACAGTTGGCAGCTGGAAGTAAAGGTCACCTTGGCTGTG
  AAGAGGACTTTGCCTGTCCACAGTGTGAATCGAGCTTTCCCAGTGAGGAAGTCCTTACTGAGCACCTTCA
  GAGCTTGCACCAGAAGCCCACAGGGGAGAAAGAGTTCAAATGCGAGAACTGCGGGAAGAAATTCCCTGTG
  AGGCAGGCCTTGCAGAGACATTTTGAGCAGCACCGGAAGGCTTGCCGAGGGGAGGCCAGGTTTGTGTGCA
  AAGCCGACAGCTGCGGGAAGAGGCTGAAAAGCAAGGATGCCCTGCGAAGGCACCAGGAAAATGTCCACAC
  CGGTGATCCTAAGAGAAAACTCATATGCTCGGTGTGCAATAGAAAATGTACCTCAGTGTCAAGCCTGCAG
  GAGCACAGGAAGATTCATGAGATATTTGATTGTCAAGAATGTATGAAAAAGTTTATTTCTGCTAATCAGC
  TGAAGCGTCACATGATTACCCACTCAGAAAAGCGGCCTTATAACTGTGAGATCTGTAACAAGTCCTTCAA
  GAGGCTCGATCAAGTGGGCGCCCACAAAGTGATCCACAGTGAGGACAAACCCTACCAGTGCAAGCTCTGT
  GGCAAGGGCTTTGCTCACAGAAACGTTTACAAGAACCACAAGAAGACCCACTCCGAGGAGAGACCTTTCC
  AGTGTGATGCATGTAAAGCCTTGTTCCGCACGCCCTTTTCTCTGCAGAGACACCTGTTAATCCACAACAG
  TGAGAGGACTTTTAAGTGTCACCACTGTGATGCCACATTTAAAAGGAAGGATACATTAAACGTTCATGTC
  CAGGTGGTCCATGAAAGACACAAGAAGTACCGATGTGAGCTGTGCAATAAGGCCTTTGTCACACCTTCAG
  TGCTTAGGAGTCATAAGAAGACACACACAGGAGAAAAGGAGAAAGTCTGCCCATATTGTGGCCAGAAATT
  TGCCAGCAGTGGGACCCTGAGAGTTCACATCCGGAGCCACACAGGTGAGCGCCCCTATCAATGCCCGTAC
  TGTGAAAAAGGTTTCAGTAAAAATGACGGACTGAAGATGCACATTCGTACTCACACCAGGGAGAAGCCCT
  ACCAGTGCTCAGAGTGCAGCAAGGCCTTCAGCCAGAAGCGGGGCCTCGATGAACACAAGAGGACACACAC
  AGGAGAAAAGCCTTTTCAGTGTGACGTATGTGACTTGGCTTTTAGCCTGAAGAAAATGCTTATTCGACAC
  AAGATGACACACAATCCTAACCGTCCGATGGCAGAGTGCCATTTCTGCCATAAGAAGTTTACAAGAAATG
  ACTACCTCAAAGTGCACATGGACAACATCCATGGGGTAGCTGACAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027547
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027547.2, NP_081823.2
• RefSeq Size: 2182 bp
• RefSeq ORF: 1800 bp
• Locus ID: 70779
• UniProt ID: Q9CXE0
• Cytogenetics: 6 C1
• Gene Summary: Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes (By similarity). May regulate the expression of proteins involved in extracellular matrix development and maintenance, connective tissue components and molecules regulating cell migration and adhesion. May cause G2/M arrest and apoptosis in cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).