Otop3 (NM_027132) Mouse Untagged Clone

CAT#: MC219533

Otop3 (untagged) - Mouse otopetrin 3 (Otop3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Otop3
• Synonyms: 2310011E08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219533 representing NM_027132
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGCAGACTTCAGCCCCTGCTGAGCCTGCACCCATGCCTTCTCCAGAAGCAAAGACCACTGAGG
  GAGCATCCTCCTATGACCAAGCAGACATGGAGACTAAGCATGCGGGGTCCCCATGCCCCCCGAAGCAGAA
  GTCCTGGCTGGCTCGGCATTTCTCCCTGCTGTTGCGGAGAGACCGACAGGCCCAGAAGGCCGGGCAGCTC
  TTCTCCGGGCTCCTGGCTCTCAATGTGGTATTCCTGGGTGGGGCTTTTATCTGTAGCATGATATTCAACA
  AGGTGTCTGTCACTCTGGGGGACGTGTGGATCCTGCTGGCTGCACTGAAGGTCCTCTCCCTGTTGTGGCT
  CCTCTACTACACAGTGGGTACCACCCGGAAGCCCCACGCAGTCCTGTACCGTGATCCCCACGCGGGACCC
  ATCTGGGTGAGGGGCTCCCTGGTGCTCTTCGGCAGCTGTACCGTCTGCCTGAACATATTTCGAATGGGTT
  ATGACGTGAGCCACATTCACTGCAAGTCAGAAGTGGAACTCATCTTTCCGGCCATTGAGATTGTCTTTAT
  GATTATTCAGACATGGGTGCTCTGGAGACACTGTAAGGACTGTGTTCAGGTGCAGACCAACTTCACCAGG
  TGTGGACTCATGCTGACTCTGGCCACAAACTTGCTGATGTGGGTTTTGGCTGTGACCAATGACTCCATGC
  ACCGTGAGATTGAGGCTGAGCTCGACGCCCTCATGGAGAAGTTCTCAGTTCTTCAGTGCAGGACCTGCCT
  GAGATGCTGTCTTTTCCTGCCCTATCTCCTCTCAGGTAACGGGACCAACACTTGCATGTGCCTCAACACC
  ACAGTGTGCGAGGTCTTCCGGAAGGGCTATCTGATGCTCTACCCCTTCAGCACTGAGTACTGCCTCATCT
  GCTGTGCTGTGCTCTTCGTCATGTGGAAGAACGTGAGCCGCAGCCTGGCAGCCCACACAGGTGCCCATCC
  CAACAGATCACCCTTCCGCCTGCACGGGACCATCTTTGGGCCACTGCTGGGCCTTTTGGCACTGGTGGCT
  GGCGTATGTGTCTTTGTGCTCTTCCAAATCGAAGCGAGTGGCCCTGACATAGCCCGCCAGTACTTCACCC
  TCTACTATGCGTTCTATGTGGCTGTGCTGCCCACCATGAGCCTGGCGTGCCTGGCTGGTACAGCCATCCA
  TGGGCTGGAGGAGCGCGAATTGGACACGCTCAAAAACCCCACACGCAGCCTGGATGTGGTGCTGTTGATG
  GGCGCTGCACTGGGCCAGATGGGCATCGCCTACTTCTCCATCGTGGCTATCGTGGCCACCCAGCCGCACG
  AGCTACTCAACCAGCTTATCCTGGCCTACTCACTGCTGCTAATCCTGCAGCACATCACTCAGAATCTCTT
  CATCATCGAGGGCCTGCACCGCCGCCCCCTCTGGGAGCCTGCGGTCTCAGGTGTGATGGAGAAGCAGGAT
  GTTGAGCTACCCCGCAGAGGCTCCCTGCGGGAGCTGGGTCAGGACCTACGGAGAGCCTCAAGGGCCTACA
  TCCACTCCTTCAGCCACCTCAACTGGAAACGCAGGATGCTCAAGGAGATCTCTCTCTTCCTCATCCTCTG
  CAACATCACACTGTGGATGATGCCCGCCTTCGGCATACACCCAGAGTTTGAGAACGGGCTGGAAAAGGAT
  TTTTACGGCTACAGGACCTGGTTCACCATTGTTAACTTCGGCCTGCCCCTGGGTGTGTTCTACCGCATGC
  ACTCTGTCGGGGGGCTGGTGGAGGTCTACCTGGGGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027132
• Insert Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027132.2, NP_081408.2
• RefSeq Size: 2422 bp
• RefSeq ORF: 1791 bp
• Locus ID: 69602
• Cytogenetics: 11 E2
• Gene Summary: Proton-selective channel that specifically transports protons into cells. Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes.[UniProtKB/Swiss-Prot Function]