Net1 (NM_019671) Mouse Untagged Clone

CAT#: MC219526

Net1 (untagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Net1
• Synonyms: mNET1; Net1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219526 representing NM_019671
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCAGAGCCGGCGGCTCAGAAGCAGCCCCGACCTCGGAGGAGAAGCCGCAGGGTCTCAATGCTCA
  GCGAGGAACCTGCCGCGGGGCTTCCGGCCGACACACCCGGGCCGGCGGCGAACGAGAGATGCTCCCTTCG
  GAGAGGCAGCTCCTTCACATTTTTAACCCCTGGCCCACACTGGGACTTCACTTTGAAAAGAAAACGAAGA
  GAGAAAGATGATGATGCTGTAAGCCTTAGCAGCCTTGATTTGAAGGAGCCAAGCAATAAGAGAGTTCGGC
  CTCTAGCTCGGGTCACATCTTTGGCAAACTTAATCTCTCCCGTAAGAAATGGAGCAGTCAGGCGCTTCGG
  TCAGACCATACAGTCGTTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTCATTCAGC
  AGGTCCACCGTCCCCACACCCACCAAGAGAAGAAGCAGTGCACTATGGTCGGAAATGCTAGACATCAATA
  TGAAGGAGTCCTTGACCACTCGAGAGATCAAACGTCAGGAGGCCATATATGAATTGTCCCGAGGGGAACA
  GGACTTGATTGAGGATCTCAAGCTCGCTAGAAAGGCCTACCATGACCCCATGTTAAAGCTGTCTATCATG
  TCAGAAGAGGAACTCACGCACATATTTGGAGACTTGGATGCTTATATACCTCTGCACGAAGATCTGTTAG
  CAAGAATTGGAGAAGCAACCAAGCCTGATGGGACTGTGGAGCAGATCGGTCACATTCTCGTGAACTGGTT
  ACCAGGCCTGAATGCCTACAGAGGCTACTGCAGTAACCAGCTGGCGGCCAAGGCTCTTCTGGATCAGAAG
  AAACAAGACCCAAGAGTCCAAGACTTCCTCCAGCGCTGTCTGGAGTCTCCTTTCAGTCGAAAACTAGATC
  TTTGGAGTTTCCTAGATATCCCTCGAAGTCGCCTCGTCAAGTACCCCTTACTGTTAAAAGAAATCCTTAG
  ACACACTCCCAAAGACCACCGTGATGTTCAGCTTCTAGAAGAGGCAATATTGATAATACAAGGAGTTCTT
  TCTGATATCAACTTGAAGAAAGGCGAATCTGAGTGTCAGTATTACATCAACAAGCTGGAGTACCTGGACG
  AGAAGCAGAAGGACCCTAGGATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGAAGAATAAAAG
  CGGCCATAAACTGTACATTTTCCTATTTCAAGACATCTTGGTTTTGACTCGGCCTGTCACCCGAAATGAG
  CGCCACTTGTACCAGGTTTACCGGCAGCCCATCCCAGTCCAGGAGCTGGTGCTGGAAGACCTGCAGGACG
  GGGATGTGCGCATGGGTGGCTCCTTCCGAGGGGCCTTCGGCAACTCAGACAAAGCTAAAAATATCTTTAG
  GGTCCGCTTCCAAGACCCTTCTCCAGGCCACTCCCACACACTGCAAGCCAATGATGTGTTCCATAAGCAG
  CAGTGGTTCAACTGTATTCGCGCGGCCATTGCCCCCTTCCAGCGGGCCGCCAGCCCCTTAGAGCTTCAGG
  GCTTGCCAGACCTCCACGAAGAGTGTGAAGAGAACAACCCTTCTGCTGGGAACCTCAGAGCCCAGCGACG
  GTCATGCGTGGTTCCTGGTGTCATGCAGATAGACGAGGAAAGCGCCCTGGATTGTGGCTCCAGTGTACAG
  ACAGTGGAAGACACCAGGAACATGAAGGCACAGCGGCCTCAGCCTGGCTTACGAAGAGCAAGGGACAAAG
  CACAGTCAGGTGGCAAAAAGAAAGAGACTTTGGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019671
• Insert Size: 1788 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019671.3, NP_062645.2
• RefSeq Size: 3995 bp
• RefSeq ORF: 1788 bp
• Locus ID: 56349
• UniProt ID: Q9Z206
• Cytogenetics: 13 A1
• Gene Summary: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway. Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).