Otop1 (NM_172709) Mouse Untagged Clone

CAT#: MC219521

Otop1 (untagged) - Mouse otopetrin 1 (Otop1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Otop1
• Synonyms: A530025J20Rik; Otp1; tlt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219521 representing NM_172709
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGGGGCCCGGGCGCGCCCTCTTCTCCCGCAGCATCCTCAGGCTCCTCGCGTGCCGCGCCGTCGG
  GGATCGCGGCATGCCCTCTGTCCCCACCTCCCCTAGCCCGAGGGTCTCCACAAGCTTCCGGTCCCCGGCG
  CGGCGCCAGCGTCCCGCAGAAGCTGGCGGAGACTCTGAGCAGCCAGTATGGACTGAACGTGTTCGTGGCG
  GGGCTGCTGTTCCTGCTGGCCTGGGCGGTGCACGCGACGGGTGTGGGCAAGAGCGACCTGCTGTGCGTTC
  TAACCGCGCTTATGCTGCTGCAGCTGCTCTGGATGCTGTGGTACGTGGGTCGCAGCTACATGCAGCGCCG
  CCTCATCCGCCCCAAGGACACGCACGCGGGTGCGCGCTGGCTTCGCGGAAGCATCACGTTGTTCGCGTTT
  ATCACTGTCGTCCTGGGATGCTTGAAAGTCGCATACTTCATTGGATTCTCGGAGTGCTTGTCAGCCACCG
  AGGGAGTTTTCCCAGTCACCCATGCAGTGCATACCCTATTGCAGGTGTATTTCCTCTGGGGCCATGCTAA
  GGATATCATCATGTCTTTCAAAACACTGGAAAGGTTTGGGGTGATCCATTCAGTGTTCACGAACCTCCTA
  CTGTGGGCCAACAGCGTCCTGAATGAATCAAAGCACCAGCTGAATGAGCACAAGGAACGGCTGATCACTC
  TGGGCTTTGGCAACATCACCATCGTTTTGGATGACCACACACCACAGTGTAACTGCACACCACCCGCCCT
  CTGCTCTGCCCTCTCCCATGGGATTTACTATCTGTACCCCTTCAACATTGAGTACCAGATCCTGGCCTCG
  ACCATGCTCTACGTGCTGTGGAAGAACATCGGGCGCAGAGTGGACAGCTCCCAGCACCAGAAGATGCAGT
  GCAGATTCGACGGGGTCCTAGTGGGCTCCGTGCTGGGCTTGACAGTGCTGGCTGCCACCATCGCCGTGGT
  TGTGGTGTACATGATCCACATCGGGCGCTCCAAATCCAAGAGCGAGTCGGCTCTCATCATGTTCTATTTG
  TACGCTATCACGGTGCTGCTGCTTATGGGGGCCGCAGGGCTAGTCGGAAGCTGGATTTACAGGGTGGATG
  AGAAGTCTCTGGATGAGTCAAAGAACCCAGCGCGCAAGCTGGATGTTGACCTGTTGGTGGCCACCGGCTC
  CGGCTCCTGGCTCCTCTCCTGGGGCTCCATTCTGGCCATCGCCTGTGCTGAGACTCGCCCACCGTACACC
  TGGTACAACCTGCCCTACTCGGTCCTGGTGATCGTGGAGAAGTATGTCCAAAACATTTTCATCATCGAGT
  CCGTGCACCTCGAGCCTGAGGGGGTCCCGGAGGATGTGCGCACTCTGCGTGTGGTCACCGTCTGCAGCAG
  CGAGGCTGCCGCACTGGCTGCATCCACTCTCGGGAGCCAGGGGATGGCCCAGGATGGGTCACCTGCTGTC
  AATGGAAATCTGTGTCTGCAGCAGAGGTGTGGGAAAGAGGACCAGGAGTCTGGCTGGGAAGGAGCTACGG
  GGACAACCCGATGTCTGGACTTCCTTCAGGGCGGCATGAAGAGGAGGCTTCTCAGAAACATCACGGCCTT
  TCTGTTTCTTTGCAACATCTCGCTTTGGATTCCCCCTGCCTTTGGCTGCCGTCCCGAGTATGACAACGGA
  TTGGAGGAAATTGTCTTTGGCTTTGAACCCTGGATAATTGTGGTCAACCTGGCCATGCCCTTTTCCATTT
  TCTACCGGATGCACGCAGCTGCTGCCCTCTTTGAGGTCTATTGTAAGATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172709
• Insert Size: 1803 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172709.3, NP_766297.2
• RefSeq Size: 3171 bp
• RefSeq ORF: 1803 bp
• Locus ID: 21906
• UniProt ID: Q80VM9
• Cytogenetics: 5 20.35 cM
• Gene Summary: Proton-selective channel that specifically transports protons into cells (PubMed:29371428). Proton channel activity is only weakly-sensitive to voltage (PubMed:29371428). Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes (PubMed:29371428). In the vestibular system of the inner ear, required for the formation and function of otoconia, which are calcium carbonate crystals that sense gravity and acceleration (PubMed:12651873). Probably acts by maintaining the pH appropriate for formation of otoconia (PubMed:29371428). Regulates purinergic control of intracellular calcium in vestibular supporting cells (PubMed:17606897, PubMed:20554841). May be involved in sour taste perception in sour taste cells by mediating entry of protons within the cytosol (PubMed:29371428). Also involved in energy metabolism, by reducing adipose tissue inflammation and protecting from obesity-induced metabolic dysfunction (PubMed:24379350).[UniProtKB/Swiss-Prot Function]