Ptgs1 (NM_008969) Mouse Untagged Clone

CAT#: MC219515

Ptgs1 (untagged) - Mouse prostaglandin-endoperoxide synthase 1 (Ptgs1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptgs1
• Synonyms: COX; Cox-; Cox-1; Cox-3; COX1; Pgh; PGHS-1; Pghs1; PHS 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219515 representing NM_008969
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCGAAGGAGTCTCTCGCTCTGGTTTCCCCTGCTGCTGCTCCTGCTGCTGCCGCCGACACCCTCGG
  TCCTGCTCGCAGATCCTGGGGTGCCCTCACCAGTCAATCCCTGTTGTTACTATCCGTGCCAGAACCAGGG
  TGTCTGTGTCCGCTTTGGCCTCGACAACTACCAGTGTGATTGTACTCGCACGGGCTACTCAGGCCCCAAC
  TGTACCATCCCTGAGATCTGGACCTGGCTTCGGAATTCTCTGCGGCCCAGCCCCTCGTTCACCCATTTCC
  TGCTGACACATGGATACTGGCTCTGGGAATTTGTGAATGCCACCTTCATCCGAGAAGTACTCATGCGCCT
  GGTACTCACAGTGCGGTCCAACCTTATCCCCAGCCCTCCGACCTACAACTCAGCGCATGACTACATCAGC
  TGGGAGTCCTTCTCCAATGTGAGCTACTATACTCGCATTCTGCCCTCTGTACCCAAAGACTGCCCCACAC
  CCATGGGGACCAAAGGGAAGAAACAGTTACCAGATGTTCAGCTTCTGGCCCAACAGCTGCTGCTGAGAAG
  GGAGTTCATTCCTGCCCCCCAGGGCACCAACATCCTGTTTGCCTTCTTTGCACAACACTTCACCCACCAG
  TTCTTCAAGACCTCTGGAAAGATGGGTCCTGGCTTTACCAAGGCCTTAGGCCACGGGGTAGACCTTGGCC
  ACATTTATGGAGATAATCTGGAACGACAGTATCACCTGCGGCTCTTCAAGGATGGGAAACTTAAGTACCA
  GGTGCTGGACGGAGAGGTGTACCCACCTTCCGTGGAACAGGCGTCCGTGTTGATGCGCTACCCACCAGGT
  GTCCCGCCTGAAAGGCAGATGGCTGTGGGCCAGGAGGTGTTTGGGTTGCTTCCGGGGCTGATGCTCTTCT
  CCACGATCTGGCTTCGTGAACATAACCGCGTGTGCGACCTGCTGAAGGAGGAGCATCCCACGTGGGATGA
  TGAGCAGCTCTTCCAGACCACTCGCCTCATCCTTATAGGAGAAACCATCAAAATTGTCATTGAGGAGTAT
  GTGCAGCACTTGAGTGGCTATTTCCTGCAGCTCAAGTTTGACCCGGAGCTGCTGTTCCGAGCCCAGTTCC
  AATATCGAAACCGCATCGCCATGGAATTTAACCATCTCTATCACTGGCATCCACTCATGCCCAACTCCTT
  CCAAGTGGGCTCACAAGAGTACAGCTACGAGCAGTTTTTATTTAACACTTCTATGCTGGTGGACTATGGG
  GTTGAGGCACTGGTGGATGCCTTCTCTCGCCAGAGGGCTGGCCGGATTGGTGGAGGTAGGAACTTTGACT
  ATCATGTTCTGCATGTGGCTGTGGATGTCATCAAGGAGTCCCGAGAGATGCGCCTACAGCCCTTCAATGA
  ATACCGAAAGAGGTTTGGCTTGAAGCCTTACACCTCTTTCCAGGAGCTCACAGGAGAGAAGGAGATGGCT
  GCTGAGTTGGAGGAGCTGTACGGTGACATCGATGCTTTAGAGTTCTACCCGGGGTTGCTGCTGGAGAAGT
  GCCAGCCCAACTCCATCTTTGGAGAAAGTATGATAGAGATGGGGGCTCCCTTTTCCCTCAAGGGCCTCCT
  AGGGAATCCCATCTGTTCCCCAGAGTACTGGAAACCCAGCACGTTCGGTGGTGACGTGGGCTTCAACCTT
  GTCAACACAGCCTCACTGAAGAAACTGGTCTGCCTCAACACCAAGACCTGCCCCTATGTTTCCTTCCGTG
  TGCCAGATTACCCTGGAGATGACGGGTCTGTCTTAGTGAGACGCTCCACTGAGCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008969
• Insert Size: 1809 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008969.3, NP_032995.1
• RefSeq Size: 2881 bp
• RefSeq ORF: 1809 bp
• Locus ID: 19224
• UniProt ID: P22437
• Cytogenetics: 2 24.19 cM
• Gene Summary: This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. [provided by RefSeq, Jan 2014]