Prodh (NM_011172) Mouse Untagged Clone

CAT#: MC219514

Prodh (untagged) - Mouse proline dehydrogenase (Prodh), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prodh
• Synonyms: Pro-1; Pro1; Ym24d07
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219514 representing NM_011172
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCAAGCGCGTCTTCTTGCTGCGGTCGGTGGCACCACGCGTCGCTGCCCTCTCAACCAAACCGC
  AAGCCCAGGAACAGCCTCCCGCGAGCCCTGAGGCTCTTCGGGGATGTGGGGCGGCCAAGGCTGTGCGGCC
  GCCTGTGCCAGCCGTGGACTTCACCAACACGCAGGAGGCGTATCGCAGCCGGCGGAGTTGGGAGTTGGTG
  CGCAACCTGCTAGTGCTGCGGCTGTGTGCGTCGCCGGTGCTGCTAGCGCACCACGAGCAGTTGTTCCAAG
  TTGCCAGGAAGCTTCTGGGGCAAAGGATGTTCGAGAGATTGATGAAGATGACCTTCTATGGCCATTTTGT
  GGCTGGCGAGGACCAGGAGTCTATCAGGCCTCTGATCCGGCACAACAAAGCCTTTGGTGTTGGCTTTATC
  CTGGACTATGGAGTGGAGGAAGATCTGAGCCCTGAGGAGGCGGAGCGCAAAGAGATGGAGTCATGCACTT
  CTGAAGCAGAGAGAGATGGCAGTGGAGCAAATAAGAGGGAGAAGCAGTATCAGGTGCACCCCGCCTTTGG
  AGACCGCAGAGATGGTGTCATCAGTGCCCGCACCTACTTCTATGCCAATGAAGCCAAGTGTGACAACTAC
  ATGGAGAACTTACTGCAGTGCATCAAGGCCTCAGGTGGAGCCAGTGATGGTGGTTTCTCAGCCATTAAGC
  TCACTGCACTGGGGAGACCACAGTTTCTGCTGCAGTTCTCAGACGTGCTGACCAGGTGGAGACGGTTCTT
  CCATCAAATGGCTGCAGAGCAGGGACAGGCTGGGCGTGCTGCTGTAGACACAAAGCTGGAGGTGGCGGTG
  CTCCAGGACAGCATCGCAAAGATGGGCATCGCATCCAGGGCTGAGATTGAAGGGTGGTTCACGCCAGAGA
  CGCTGGGAGTGTCTGGCACCGTGGACTTGCTGGACTGGAACAGCCTCATTGACAGCAGGACCCGGCTCTC
  CAGGCACTTGGTGGTCCCCAATGTGCAGACTGGCCAGCTGGAGCCCCTGCTGTCACGGTTCACTGAGGAG
  GAAGAGCAGCAGATGAAAAGGATGCTGCAGAGGATGGATGTACTGGCCAAGAAAGCAAAAGAGGCAGGTG
  TGCGCCTGATGATTGATGCTGAGCAGAGCTACTTCCAACCAGCCATCAGCCGCCTGACCCTGGAGATGCA
  GCGCAGGTTCAATGTGGATAAGCCGTTCATCTTCAACACATTCCAGTGCTACCTCAAGGATGCCTATGAC
  AATGTGACCTTGGATATGGAACTGGCTCGCCGTGAGGGCTGGTGTTTTGGGGCCAAGCTGGTACGTGGTG
  CATACATGGCCCAAGAGCGTGTCAGGGCAGCAGAGATCGGTTATGAGGACCCCATCAACCCTACATATGA
  AGCCACCAATGCTATGTACCACAGGTGCCTTAACTATGTTCTGGAGGAGCTGAAGCACAGCACCAAGGCA
  GAGGTGATGGTGGCTTCCCACAACGAGGACACCGTGCACTTCACGTTGTGCAGGATGAAGGAGATAGGCC
  TGCATCCTGCTGATGGTCAGGTGTGCTTCGGACAGCTGCTGGGGATGTGTGACCAAATCAGCTTCCCACT
  AGGCCAGGCAGGCTTTCCTGTGTACAAGTATGTGCCCTATGGCCCTGTGATGGAGGTACTCCCTTACCTG
  TCCCGCCGTGCCCTGGAGAACAGCAGCATCATGAAGGGTGCTCAGCGAGAGAGGCAGCTGCTATGGCAGG
  AGCTCCGCAGGCGGCTGCGCACTGGCAGCCTCTTCCACCATCCGGCCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_011172
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011172.2, NP_035302.2
• RefSeq Size: 2283 bp
• RefSeq ORF: 1800 bp
• Locus ID: 19125
• UniProt ID: Q9WU79
• Cytogenetics: 16 11.19 cM
• Gene Summary: Converts proline to delta-1-pyrroline-5-carboxylate.[UniProtKB/Swiss-Prot Function]