Mtm1 (NM_001164191) Mouse Untagged Clone

CAT#: MC219506

Mtm1 (untagged) - Mouse X-linked myotubular myopathy gene 1 (Mtm1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mtm1
• Synonyms: AF073996; mKIAA4176; Mtm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219506 representing NM_001164191
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTGCATCAGCATCTAAGTATAATTCACACTCCTTGGAGAATGAATCCATTAAGAAAGTGTCTC
  AAGATGGAGTCAGTCAGGATGTGAGTGAGACTGTCCCTCGGCTCCCAGGGGAGTTACTAATTACTGAAAA
  AGAAGTTATTTACATATGTCCTTTCAATGGCCCCATTAAGGGAAGAGTTTACATCACAAATTATCGTCTT
  TATTTAAGAAGTTTGGAAACGGATTCTGCTCTAATACTTGATGTTCCTCTGGGTGTGATATCAAGAATTG
  AAAAAATGGGAGGCGCGACAAGTAGAGGAGAAAATTCCTATGGTCTAGATATTACTTGTAAAGATTTGAG
  AAACCTGAGGTTTGCATTGAAGCAAGAAGGCCACAGCAGAAGAGATATGTTTGAGATCCTTGTAAAACAT
  GCCTTTCCTCTGGCACACAATCTGCCATTATTTGCATTTGTAAATGAAGAGAAGTTTAACGTGGATGGGT
  GGACTGTTTATAATCCAGTTGAAGAATATAGAAGGCAGGGCCTGCCCAATCACCATTGGAGGATAAGTTT
  TATTAACAAGTGCTATGAGCTCTGTGAGACATACCCTGCTCTTTTGGTGGTTCCCTATCGGACCTCAGAT
  GATGATCTTAGGAGGATCGCAACGTTTAGATCCCGAAATCGGCTTCCTGTACTGTCGTGGATTCACCCAG
  AAAACAAAATGGTCATTATGCGCTGCAGTCAGCCTCTTGTCGGTATGAGTGGTAAAAGAAATAAAGATGA
  CGAGAAATACCTGGATGTGATCAGGGAAACTAACAAACAAACTTCTAAGCTCATGATTTATGATGCACGA
  CCCAGTGTAAATGCAGTCGCCAACAAGGCAACAGGAGGAGGATATGAAAGTGATGACGCATATCAAAACT
  CAGAACTTTCCTTCTTAGACATTCATAATATTCATGTTATGCGAGAATCTTTAAAAAAAGTGAAAGATAT
  TGTTTATCCCAACATAGAAGAATCTCATTGGTTGTCCAGTTTGGAGTCTACTCATTGGTTAGAACATATC
  AAGCTTGTTCTGACCGGTGCCATTCAAGTGGCAGACCAAGTGTCTTCAGGAAAGAGCTCGGTACTTGTGC
  ACTGCAGTGACGGATGGGACAGGACCGCTCAGCTGACATCCTTGGCCATGCTGATGTTGGACAGCTTCTA
  CAGAACTATTGAAGGCTTTGAGATATTGGTACAGAAAGAGTGGATAAGTTTTGGCCATAAATTTGCATCT
  AGAATAGGTCATGGTGATAAAAACCATGCTGATGCTGATCGATCTCCTATTTTTCTTCAGTTTATTGACT
  GTGTGTGGCAGATGTCGAAACAGTTCCCCACAGCTTTTGAGTTCAATGAAGGCTTTTTGATTACCGTTTT
  GGATCATCTGTATAGCTGTCGATTTGGTACTTTCTTATTCAACTGTGACTCGGCTCGAGAAAGACAGAAA
  CTTACAGAAAGAACAGTTTCTCTATGGTCGCTAATTAACAGCAATAAAGACAAATTCAAAAACCCCTTCT
  ATACAAAAGAAATCAATCGGGTTTTGTATCCAGTTGCCAGCATGCGTCACTTGGAACTGTGGGTGAATTA
  TTACATCCGATGGAATCCCAGGGTCAAGCAGCAACAGCCCAACCCAGTGGAGCAGCGTTACATGGAGCTT
  TTGGCCTTGCGTGACGATTATATAAAGAGGCTCGAGGAATTGCAGCTGGCCAACTCCGCCAAGCTTGCTG
  ATGCCCCCGCTTCGACTTCCAGTTCGTCACAGATGGTGCCCCATGTGCAGACGCACTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164191
• Insert Size: 1812 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164191.1, NP_001157663.1
• RefSeq Size: 3379 bp
• RefSeq ORF: 1812 bp
• Locus ID: 17772
• UniProt ID: Q9Z2C5
• Cytogenetics: X 36.55 cM
• Gene Summary: Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine-containing peptides. Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome. Plays a role in vacuolar formation and morphology (By similarity). Regulates desmin intermediate filament assembly and architecture. Plays a role in mitochondrial morphology and positioning (PubMed:21135508). Required for skeletal muscle maintenance but not for myogenesis (PubMed:12391329). In skeletal muscles, stabilizes MTMR12 protein levels (PubMed:23818870).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.