Lmnb2 (NM_010722) Mouse Untagged Clone

CAT#: MC219504

Lmnb2 (untagged) - Mouse lamin B2 (Lmnb2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lmnb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219504 representing NM_010722
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTCTGCCGCCCCACGCGGGGCCCGCCACGCCGCTGTCGCCCACGCGTCTGTCTCGGCTGCAGG
  AGAAGGAGGAGCTCCGTGAGCTCAACGACCGCCTGGCACACTACATCGACCGTGTCCGCGCGCTGGAGCT
  GGAGAATGATAGGTTGCTGCTCCGGATCTCCGAGAAGGAGGAGGTGACCACTCGCGAGGTGAGTGGCATC
  AAGACCCTGTACGAGTCAGAGCTGGCTGACGCCCGACGGGTACTGGATGAGACGGCCCGTGAACGTGCCC
  GGCTGCAGATTGAAATTGGAAAGGTGCAGGCTGAGCTAGAGGAGGCCAGGAAGAGTGCCAAGAAGCGGGA
  AGGTGAGCTCACAGTGGCCCAGGGCCGAGTGAAGGATCTGGAATCACTGTTTCACCGGAGTGAGGCTGAG
  CTGGCCACAGCCCTCAGTGACAAGCAGGGCCTGGAGACAGAGGTGGCAGAGCTTCGAGCACAGCTGGCCA
  AGGCAGAAGATGGTCATGCTGTGGCCAAGAAGCAGTTGGAGAAGGAGACGCTGATGCGTGTGGACCTGGA
  GAACCGATGCCAGAGCCTGCAGGAGGAGCTGGCTTTCAGCAAGAGTGTGTTTGAGGAGGAGGTACGGGAG
  ACCCGACGGAGGCACGAGCGGCGCTTGGTGGAGGTGGACAGCAGCCGGCAACAGGAATATGACTTCAAGA
  TGGCTCAGGCCCTGGAGGACCTGCGCAGTCAGCACGATGAGCAAGTGCGCCTGTACCGGGTAGAACTGGA
  GCAGACCTACCAGGCCAAGCTGGACAACGCCAAGCTGCTCTCGGACCAGAATGACAAGGCAGCCCATGCA
  GCCCGCGAGGAGCTCAAGGAGGCCCGCATGCGCGTGGAGTCCCTCAGCTACCAGCTTTTAGGCCTCCAAA
  AGCAGGCCAGTGCTGCAGAGAACCACATCCATGAGCTGGAGGAGGCCTTGGCTGGGGAGCGTGACAAGTT
  CCGCAAGATGCTGGACGCTAAGGAACAGGAGATGACGGAGGTGCGGGACGCCATGCAGCAGCAGCTGGCA
  GAGTACCAAGAGCTGCTGGACATTAAGCTGGCCCTGGACATGGAGATAAGCGCCTACCGCAAGCTGCTGG
  AGGGCGAGGAGGAGAGGCTGAAGCTGTCTCCCAGCCCTTCATCACGGATCACCATCTCTCGGGCCACATC
  GAGCAGCAGCAGCAGCAGCGGGGTTGGCATGTCTGTGGGCCAGGGCCGGGGCAAGCGCCGGCGGCTGGAG
  ACTGAGGACACCTCAGGCTCACCCAGCAGGGCTTCCAGAGTGAGCAGCGGCTCCCGCCTGGCTCAGCAGA
  CTGTGGCCACGGGTGTTGTGAACATCGACGAGGTGGACCCAGAGGGCAGGTTCGTGCGCCTTAAGAACTC
  TTCAGACAAGGACCAGTCTTTGGGGAACTGGAGGATCAAGAGACAGGTTCTGGAGGGTGAGGACATTGCC
  TACAAGTTCACACCCAAGTATGTCCTGCGGGCCGGCCAGACTGTCACGGTGTGGGCAGCTGGCGCAGGGG
  CTACCCACAGTCCCCCATCAACCCTTGTGTGGAAAAGCCAGACCAACTGGGGCCCTGGGGAGAGCTTCCG
  CACTGCCCTGGTCAGTGCCGACGGTGAGGAGGTGGCCGTGAAGGCTGCAAAGCACTCATCTGTCCAGGGG
  AGGGAGAACGGGGAAGAGGAGGAAGAGGAAGAGGCAGAATTCGGTGAAGAGGACCTCTTCCACCAGCAGG
  GGGACCCAAGGACTACCTCAAGGGGCTGCCGACTGATGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_010722
• Insert Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010722.5, NP_034852.3
• RefSeq Size: 3389 bp
• RefSeq ORF: 1848 bp
• Locus ID: 16907
• UniProt ID: P21619
• Cytogenetics: 10 39.72 cM
• Gene Summary: This gene encodes a protein component of the nuclear lamina, which provides a structural framework for the nuclear envelope. Defects in this gene were found to cause abnormalities in the shape of neurons. This locus represents one of two B-type lamin genes that may be partially, but not entirely, functionally redundant in neuronal development. Loss of both B-type lamin genes in keratinocytes results in ichthyosis and a skin barrier defect leading to dehydration. Alternative transcriptional initiation and splicing results in multiple transcript variants and protein isoforms, including an isoform with a shorter N-terminal rod domain that may function in nuclear envelope remodeling during spermatogenesis. A related pseudogene is found on chromosome 5. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: An in-frame AUG is located 19 codons upstream of the annotated translation start site but is not being annotated as a start site since it is in a weak Kozak sequence context and is probably too close to the 5' end of the transcript to be used as a translation start site. CCDS Note: The coding region has been updated to start at an alternate in-frame start codon that is more supported by available transcript data.