Cfi (NM_007686) Mouse Untagged Clone

CAT#: MC219492

Cfi (untagged) - Mouse complement component factor i (Cfi), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cfi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219492 representing NM_007686
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCGCTCATCTCAGTCTCTTTCTCTTGGCTCTCCACTTGAGTTCTTCCAGGTCCCCGTCAGCAT
  CAGATCTGCCTCAGGAGGAGCTGGTCGATCAGAAATGCTTACTGCAGAAATACACGCATCGCTCCTGTAA
  CAAGGTCTTCTGCCAGCCGTGGCAGAGATGTATCGAAGGCACCTGTATCTGCAAACTCCCCTACCAGTGC
  CCAAGGGCTGGCACCCCAGTGTGTGCCATGAATGGAAGGAGCTACCCGACATACTGTCACCAGAAGAGTT
  TCGAATGTCTTCACCCAGAGATCAAGTTTTCACATAATGGAACGTGCGCAGCTGAAGGAAAGTTTAATGT
  TTCCTTAATTTATGGGAGAACAAAAACAGAGGGACTTGTTCAAGTCAAACTTGTGGACCAAGATGAGAGA
  ATGTTCATATGTAAAAACAGCTGGAGCATGGCGGAGGCCAACGTGGCCTGCGTCGACCTTGGATTTCCAC
  TGGGTGTTCGTGACATACAAGGAAGTTTTAATATATCTGGAAATCTCCATATAAACGACACTGAATGCCT
  GCATGTACATTGCCGGGGAGTAGAGACCAGTTTGGCGGAGTGTGCCTTTACGAAGAGGAGAACTGAGTTG
  TCCAATGGCTTGGCGGGGGTAGTGTGTTACAAGCAGGATGCAGATTTCCCAACGAGTCTGTCCTTCCAGT
  GTGTGAATGGGAAGCACATTCCTCAGGAGAAAGCCTGCAACGGTGTCAATGACTGTGGAGACCAAAGCGA
  TGAGCTGTGTTGCAAAGGTTGCCGAGGTAACGCTTCCCTTTGTAAGTCGGGAGTTTGCATTCCAGACCAA
  TACAAGTGTAATGGTGAGGTGGACTGCATCACTGGTGAAGATGAGAGCCGTTGTGAAGAAGACCGACAGC
  AGAACATTCCAAAAGGCCTTGCACGGTCAGCTCAAGGAGAAGCTGAAATTGAAACTGAAGAAACAGAAAT
  GTTGACTCCTGGTATGGACAATGAAAGAAAACGGATAAAGTCCTTATTACCTAAACTCTCCTGTGGAGTC
  AAAAGAAACACTCACACTCGCAGGAAACGAGTGATCGGAGGGAAGCCAGCGAATGTGGGAGACTACCCAT
  GGCAGGTGGCAATTAAGGATGGCCAAAGAATCACCTGTGGTGGCATTTATATCGGTGGCTGTTGGATTCT
  GACTGCTGCGCACTGTGTCAGACCCAGTAGAGCTCACAGTTACCAAGTCTGGACGGCTTTATTAGACTGG
  CTAAAACCTAACTCTCAGTTGGGAATTCAAACGGTGAAGAGAGTTATTGTTCACGAGAAATATAATGGAG
  CCACCTTCCAAAATGACATAGCTTTGATTGAAATGAAAATGCACACGGGCAAGAAAGAATGTGAACTCCC
  CAATTCCGTCCCTGCCTGCGTCCCGTGGTCTCCATATCTGTTCCAACCGAATGACAGATGTATCATCTCT
  GGATGGGGTCGAGGGAAAGATAACCAAAAAGTCTACTCACTCAGGTGGGGCGAAGTTGATCTAATAGGCA
  ACTGCTCTCAGTTTTACCCAGATCGCTACTATGAGAAAGAGATGCAGTGCGCAGGTACGCGTGATGGGTC
  CATTGATGCCTGCAAAGGAGACTCCGGAGGCCCCTTGGTCTGTGAGGATATCAACAATGTCACTTACGTT
  TGGGGCATTGTGAGCTGGGGAGAAAATTGTGGGAAGCCCGAGTTCCCAGGTGTTTACACCAGAGTGGCCA
  ATTATTTTGATTGGATTAGCTATCACGTAGGAAGGTCTCTTGTTTCTCAACACAATGTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_007686
• Insert Size: 1812 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007686.3, NP_031712.2
• RefSeq Size: 2077 bp
• RefSeq ORF: 1812 bp
• Locus ID: 12630
• UniProt ID: Q61129
• Cytogenetics: 3 59.21 cM
• Gene Summary: This gene encodes a serine protease that plays an important role in the classical and alternative complement pathways where it cleaves C4b and C3b components of C3 and C5 convertases. The encoded preproprotein undergoes proteolytic processing to generate an active, disulfide-linked heterodimeric enzyme comprised of heavy and light chains. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).