Gga2 (NM_028758) Mouse Untagged Clone

CAT#: MC219483

Gga2 (untagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 2 (Gga2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gga2
• Synonyms: 1200007E24Rik; mKIAA1080
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219483 representing NM_028758
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGACGGCAGTGGCAGCGGGGACCGGATCCCCGGCAGGGACCGAGTCGGCGGAGGGCGGCCCTG
  GGGCAGCGGCGGCGCTGGAGCTGTGGCTCAACAAAGCCACAGACCCAAGCATGGCGGAGCAGGATTGGTC
  AGCCATCCAGAAGTTCTGTGAGCAGGTGAACACCGACCCCAGTGGCCCGACACATGCACCCTGGCTACTG
  GCCCACAAGATTCAGTCTCCACAGGAGAAGGAAGCCCTTTATGCCCTGACGGTGCTGGAGATATGCATGA
  ACCACTGTGGGGAGAAGTTCCACAGTGAAGTGGCCAAGTTCCGCTTCCTGAACGAGCTGATCAAAGTATT
  GTCCCCAAAGTACCTAGGGGCCTGGGCTACGGAGAAGGTTAAAGGAAGAGTTATTGAAATCCTCTTTAGC
  TGGACCGTCTGGTTTCCGGAAGACATCAAGATCCGAGATGCTTATCAGATGCTGAAGAAGCAAGGAATCA
  TAAAGCAAGATCCCAAACTACCGATGGATAAAATCTTGCCCCCACCGTCTCCCTGGCCGAAGAGCATCTT
  TGATGCTGATGAGGAGAAGTCAAAGCTTCTGACAAGACTTCTCAAGAGTAACCACCCTGAGGATCTTCAG
  GCTGCAAACCGGCTGATCAAGAATTTGGTCAAGGAAGAACAAGAGAAGTCTGAAAAGGTGTCCAGGCGAG
  TCAGTGCAGTAGAGGAAGTCCGCAGCCACGTGAGGGTGCTTCGGGAGATGCTGAGCATGTACCGAAGGCC
  AGGTCATGCCCTGCCCGACCAGCAGGCCCTGCAGGTTGTGTATGAAAGATGTGAGAAGCTGAGGCCCACC
  CTGTTCCGGCTTGCAAGCGACACCACTGATGACGACGATGCTCTGGCGGAGATTCTTCAAGCAAATGACC
  TTCTCACACAGGGGGTTCGGCTGTACAAACAGGTGGTGGAGGGCCGAGTTAGTGCTGGGAACGCAGTGCC
  TGCCGCAGTGGGAGCCATTCCCGCCCCCAGAGCATTCCCGAATCCAGAACCCTGTGGGTTGAACTGTCCA
  CTCATTGACCTGGAGACGCCATCTTTGCTTCATCAGGACCTGGCAGCCTTAGGAATCAACGATGTTCCCA
  CTAGGAACCAGGTTGTTATTCCGAGTTGCTGTAATGACAAGAAGCAGCCGGGTGCCATCACACTCATGGG
  GGGTGGAATCCAGAGTCTTTCTGCAGACCGGAACCTGCTGGATCTCTTCTCGCCACAGCCATCTCCAGGA
  CTGAATTATGTTCCACAAAAAAGCATCCCCAAGGAGGTGCCACCAGGTACCAAGGCCTCTCCAGGTTGGT
  CCTGGGAGGCTGGGCCATTGGCTTCTTCCACAGCCTCACAGAATACACCTCTGGCTCATGTGTTTGTCCC
  TTTGGAGTCTGTGAAACCCAGCAGCTTGCCGCCCATTGTCGTGTATGACCGGAATGGCTTCCGAATTCTG
  CTGCACTTCTCCCAGACTGGGGCCCCTGGCCACCCGGATGTGAAGGTGTTGTTGTTGACCATGATGAGCA
  CTGCCACCCAACCTGTCTGGGATGTCATGTTTCAGGTGGCTGTCCCAAAGTCAATGCGAGTGAAGCTGCA
  GCCGGCATCCAGCTCCAAGCTCCCTGCATTCAGTCCTTTGATGCCTCCAGCAGTGATATCTCAGACGCTG
  TTGCTCGACAACCCACACAAAGAACCTATCCGGTTGCGGTATAAGCTGACTTTCAACCAAGGTGGGCAGC
  CGTTCAGTGAAGTAGGAGAGGTGAAAGACTTTCCAGACCTGGCTGTCTTGAGCACCGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028758
• Insert Size: 1812 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028758.2, NP_083034.1
• RefSeq Size: 4891 bp
• RefSeq ORF: 1812 bp
• Locus ID: 74105
• UniProt ID: Q6P5E6
• Cytogenetics: 7 F2
• Gene Summary: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Regulates retrograde transport of phosphorylated form of BACE1 from endosomes to the trans-Golgi network.[UniProtKB/Swiss-Prot Function]