Ptpn6 (NM_013545) Mouse Untagged Clone

CAT#: MC219479

Ptpn6 (untagged) - Mouse protein tyrosine phosphatase, non-receptor type 6 (Ptpn6), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptpn6
• Synonyms: 70Z-SHP; hcp; Hcph; me; Ptp1C; PTPTY-42; SH-PTP1; SHP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219479 representing NM_013545
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGGTGGTTTCACCGGGACCTCAGCGGGCCTGATGCAGAGACCCTGCTGAAGGGCCGGGGAGTCC
  CTGGGAGCTTCCTGGCTCGGCCCAGCCGCAAGAACCAGGGTGACTTCTCCCTCTCAGTCAGGGTGGATGA
  TCAGGTGACTCATATTCGGATCCAGAACTCAGGGGACTTCTATGACCTGTACGGAGGGGAGAAGTTTGCG
  ACGCTGACAGAGCTGGTCGAGTATTACACGCAGCAGCAGGGCATCCTGCAGGACCGAGATGGCACCATCA
  TCCACCTTAAGTACCCACTGAACTGCTCGGACCCCACCAGTGAGAGGTGGTACCACGGCCACATATCTGG
  AGGGCAGGCGGAGTCACTGCTGCAGGCCAAGGGCGAGCCCTGGACATTTCTTGTGCGTGAGAGTCTCAGC
  CAACCTGGTGATTTTGTGCTCTCTGTGCTCAATGACCAGCCCAAGGCTGGCCCAGGTTCCCCGCTCAGGG
  TCACTCATATCAAGGTTATGTGTGAGGGTGGACGCTATACTGTGGGTGGCTCAGAGACGTTTGACAGCCT
  CACAGACCTGGTGGAGCACTTCAAGAAGACAGGGATTGAGGAGGCCTCGGGTGCCTTTGTCTACCTGCGG
  CAGCCTTACTACGCTACTCGGGTAAACGCAGCTGACATTGAGAATCGGGTCTTGGAACTGAACAAGAAGC
  AGGAGTCGGAGGACACAGCCAAGGCTGGCTTCTGGGAGGAGTTTGAGAGTCTACAAAAGCAGGAGGTAAA
  GAATCTACACCAACGTCTGGAAGGGCAGCGGCCAGAGAACAAGAGCAAGAACCGCTACAAGAACATTCTT
  CCCTTTGACCACAGCCGAGTGATCCTGCAGGGACGTGACAGTAACATCCCAGGCTCTGACTACATCAATG
  CCAACTACGTGAAGAACCAGCTGCTAGGTCCAGATGAGAACTCTAAGACCTACATCGCCAGCCAGGGCTG
  TCTGGATGCCACAGTCAATGACTTCTGGCAGATGGCTTGGCAGGAGAACACTCGTGTCATCGTCATGACT
  ACCAGAGAGGTGGAGAAAGGCCGGAACAAATGTGTCCCATACTGGCCCGAGGTGGGCACTCAGCGTGTCT
  ATGGTCTCTACTCTGTGACCAACAGTAGGGAGCATGACACAGCAGAATACAAACTGCGAACATTACAGAT
  CTCCCCACTAGACAATGGGGACCTGGTTCGGGAGATATGGCACTACCAGTACCTGAGCTGGCCTGACCAT
  GGGGTTCCCAGTGAGCCTGGGGGTGTCCTCAGCTTTCTGGATCAGATCAACCAGCGACAGGAAAGTTTGC
  CTCATGCAGGGCCCATCATTGTGCATTGCAGCGCTGGCATCGGCCGCACGGGCACCATCATCGTCATTGA
  TATGCTTATGGAAAGCATCTCCACCAAGGGGCTAGACTGTGACATTGATATCCAGAAGACCATCCAGATG
  GTACGAGCACAGCGCTCCGGCATGGTGCAGACCGAGGCCCAGTACAAGTTTATTTACGTGGCCATTGCCC
  AGTTCATCGAAACGACCAAGAAGAAACTGGAGATCATACAATCCCAGAAGGGCCAGGAGTCGGAGTATGG
  GAATATCACGTACCCTCCCGCTGTGAGGAGTGCCCACGCCAAAGCCTCGCGTACTTCCTCCAAGCACAAG
  GAGGAGGTGTACGAAAACGTGCATAGCAAGAGCAAGAAGGAAGAGAAAGTAAAGAAGCAGCGGTCGGCAG
  ACAAGGAGAAGAACAAAGGTTCTCTCAAGAGGAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013545
• Insert Size: 1788 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013545.3, NP_038573.2
• RefSeq Size: 2219 bp
• RefSeq ORF: 1788 bp
• Locus ID: 15170
• UniProt ID: P29351
• Cytogenetics: 6 59.17 cM
• Gene Summary: Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 2. It encodes isoform a, which has a shorter and distinct N-terminus, compared to isoform b.