C8a (NM_146148) Mouse Untagged Clone

CAT#: MC219464

C8a (untagged) - Mouse complement component 8, alpha polypeptide (C8a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: C8a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219464 representing NM_146148
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGTGGTTGCTTTCTTTGGCTTGTCTTTGGTGGCATGGCACCCTGGGGTAACTGCACAAGAGAAGG
  TGAACCAGCGGGTCACCCGAGCTGTGACCCCGCAAGCAGTGTCCTGTCAGTTGAGTGACTGGTACAAATG
  GACAGATTGTTTTCCATGTCAGGATAAAAAGTACCGATACCGGAGCCTCTTGCAGCCAAGCAAGTTTGGA
  GGAACCATCTGCAGTGGTGATATCTGGGATGAGGCCAGCTGTGACAGCCCCACACCTTGTCTAAGGCAAG
  CACAGTGTGGACAGGATTTCCAGTGTAGAGAGACAGGTCGCTGCCTGAAACGCCACCTGGTGTGTAACGG
  AGATAACGACTGCCTGGATGGCTCTGATGAGTCCGACTGTGAAGATGTCAGGGTCACTGAGGATGATTGC
  CATCAGTATGAACCAATCCCAGGATCAGAAAGGGCAGCCTTGGGGTACAACATCCTGACTCAAGAGGAAG
  CTCAGAGTGTTTACGATGCTAAGTATTATGGGGGCCAGTGTGAAACTGTGTACAATGGAGACTGGAGAAA
  GCTGCGGTACGACCCCACCTGTGAGCGCCTCTACTATGGGGAGGATGAGAAATACTTTCGGAAGCCCTAC
  AACTTCCTTAAGTACCACTTTGAAGCCCTGGCGGATACTTCAATCTCCTCAGAGTTTTATGATGATGCGA
  ATGATCTTTTTTTTCACATAAAGAATGGCAAGTCCCACTCAGCTGGTGTAACCGTTGGCGTAGCCCCAGT
  AAAAAGTCCTGTCTCAATAGAGGTGACTGGATCCGGGTCCAAAGCATCTTCATTCCTGAACAAGTTAAAC
  AAGTATAATGAGAAGAGATATGGCTTCATGAGAGTGTCTACAAAGATACAGACTGCACAGTTTAAGATGA
  GAAGGAACAATATTGTGCTGGATGAAGGAATGCTGCAGTCATTAATGGAGCTCCCAGAGCAGTTCAACTA
  TGGCATGTACGCCAAGTTCATCAATGACTATGGCACCCACTACATCACCTCTGGAACCATGGGTGGCATT
  TATGAATATGTTATGGTGCTTGACAAAGAGAAAATGAAAACTGAAGGTACTACTGTGGATGAAGTCCAAA
  AATGCATTGGCGGTGGCATTGGCATAGGAATTAAGGATAGTACAATTGAAGGAGTAGGTATATCAGGAGA
  GTTTTGTGAAAACTCTGGAGATGGTGACCGTGACATAAGGAAGAAAATCACAGGAGTGGAGGACATCATT
  TCTCGGGTGCAAGGTGGAAGTTCTGTCTGGGGCAGTGTCTTGACACACAACAGCAGTGCCATTACATATC
  AGTCCTGGGGGAGATCGTTAAAGTATAACCCTGTTGTTATCGATTTTGAGATGCAGCCTATCTACCAGCT
  ACTGCGGCACACAAACTTGGGACCCCTGGAGACGAAACGTCAGAACCTGCGACGGGCTTTGGACCAGTAC
  CTGATGGAATTTAATGCCTGTCGTTGTGGGCCCTGCTTCAACAATGGGGAACCCATACTTGACGGCACCA
  ACTGCCGGTGTCAGTGTAGTATGGGCCGGCAGGGCCTGGCCTGTGAACGCACGGTGATAGAGGGGCTCAA
  AGACTTCAAAGCCGCTGGCCACTGGAGCTGCTGGAGTTCCTGGTCAGAGTGCAGAGGAGGTTCCCAGGAG
  AGGAGGAGACAGTGCAACAACCCACCACCCAAGAATGGAGGTACCCCATGCCTAGGGAGGAACCTGCAGA
  CCCAGGCCTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146148
• Insert Size: 1764 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146148.2, NP_666260.1
• RefSeq Size: 3636 bp
• RefSeq ORF: 1764 bp
• Locus ID: 230558
• UniProt ID: Q8K182
• Cytogenetics: 4 C6
• Gene Summary: This gene encodes the alpha subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the alpha subunit, which associates with the beta and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the beta subunit. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).