Med26 (NM_027485) Mouse Untagged Clone

CAT#: MC219419

Med26 (untagged) - Mouse mediator complex subunit 26 (Med26), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med26
• Synonyms: 5730493L18Rik; AI414941; AW495270; Crsp7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219419 representing NM_027485
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCGGCTCCGGCGTCTCCGCAGCAGATGAGGGACCGGCTGCTACAGGCCATCGACTCCCAGAGCA
  ACATCCGGAACATGGTGGCAGTGTTGGAAGTGATCTCCAGTCTGGAGAGATACCCCATCACCAAAGAGGC
  ACTGGAGGAGACACGACTAGGGAAGCTCATCAATGATGTCCGCAAGAAAACCAAGAATGAGGAGCTGGCC
  AAGCGGGCCAAGAGGCTGCTACGAAGCTGGCAGAAGCTCATCGAGCCTGTACACCAGAACGAAGTGGCAC
  TACGGGCACTGGCAGGAGCTGCAGGCTCTGCCAATGGGGGTGCGCACAACTGCCGACCAGAGATGGGAGT
  GGCTGGTGCACCCAAGAGCATCCATGACCTGAAGAATCGCAACGACATCCAAAGGCTCCCTGGGCAACGG
  CTAGATAGGCTGGGCAGCCGTAAGCGCAGGGGGGACCAGCGTGACCTGGGCCACCCAGGGCCACCCCACA
  AAGTTTCCAAAGGCAGTCCTGACCCTCTGGTTCCCAATGCATCCCCTCTCCCCACCAATGGGATCAGCGG
  GAGCCCAGAGAGCTTACCCAGCCCCCTGGATGGCAGTGGGCACTTGGGCCCTGATGGCAGCCGCCTGGAG
  CCCAGTGATAATGAGAAGCACAGCACCAAGATTCCCGTGAACGCCGTGCGGCCGCGCCCCAGCTCTCCCG
  GCTTGGGCAAACCCCCAGTTCCCTGCCTGCAGACCAAGGCTGCACAGTTGCAGCAGTTGGACAGGGCAGA
  TGAGTCCCCAGGCCCTCCCTATCCCAGGGGGTCCTCTCGCTGCTCCTTCAGCCCCCGGAACTCACGGCAC
  GAAGGCTCCTTTTCACGGCATCGGAGCTCTTACATACCCAAGGGCCAGGTGTCCAGCCCTTCCCCATGGC
  CCCAGCCACCGGACAACACGCAGGTACCATCCCCACTCCCGCTGGCCCAGCCACCCACGCCCCCTGTGCG
  CCGTCAGGAGCTGCTGCCTAATGCTGAGAGCCCTGTGCACTGGCCAGAGCAGTCTGAGGGCCACCCACGG
  CTAACGGGGCCAGCCTGCAGGGCTGGATTTTCTCCAGATTCCTCCAAGGCGGACAGTGATGCTACCTCTT
  CGGGTGGCTCGGACAGCAAAAAGAAAAAGAGGTACCGACCTCGAGACTACACAGTGAACTTGGACGGGCA
  GGTGGCTGAGGCTGGTGTTAAACCTGTGCGGTTAAAAGAGCGGAAGCTCACCTTTGACCCCATGACAAGA
  CAGATCAGGCCTCTGACCCAGAAAGAGCCGGTGCGGGCTGACAGCCCAGTACCCACAGAGCAGCTGCCTA
  GGACAGAGCTGGAGCAGCAGGAGGTCAAGGCCAGCCTCCAGAGCCCTTTTGAGCAGACGAACTGGAAGGA
  GCTATCACGAAATGAGATCATACAGTCCTACCTGAGCCGCCAGAGCAGCTTGCTATCCTCCTCAGGCGCA
  CAGACCCCAGGTGCGCACCACTTTATGGCTGAATACCTGAAGCAGGAGGAGAGCAGTCGGCAAGGGGCCC
  GGCAGCCCCATGTGCTGCTACCACTGCCCACACCCACTGACCTACCAGGGCTCACACGTGAGGTCACGCA
  GGATGATCTGGACAGAATCCAGGCCCAGCAATGGCCAGGGGTTAACGGGTGTGAAGACACACAGGGTAAT
  TGGTATGACTGGACACAGTGTATATCGCTCGACCCTCATGGTGACGATGGGCGCTTGAACATTCTGCCTT
  ATGTCTGCTTGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027485
• Insert Size: 1767 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027485.4, NP_081761.2
• RefSeq Size: 3040 bp
• RefSeq ORF: 1767 bp
• Locus ID: 70625
• UniProt ID: Q7TN02
• Cytogenetics: 8 B3.3
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]