Ppp2r1a (NM_016891) Mouse Untagged Clone

CAT#: MC219405

Ppp2r1a (untagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (Ppp2r1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppp2r1a
• Synonyms: 6330556D22Rik; PP2A; PR65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219405 representing NM_016891
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGCCGACGGTGACGATTCGCTCTATCCTATTGCGGTGCTCATAGATGAACTCCGCAATGAGG
  ACGTTCAGCTTCGTCTCAATAGTATCAAGAAGCTCTCCACAATTGCCTTGGCCCTTGGGGTTGAACGGAC
  CAGAAGTGAGCTCCTGCCCTTCCTTACAGATACCATTTATGATGAAGATGAGGTCCTCTTGGCCTTGGCT
  GAACAGCTGGGAACCTTCACAACTTTGGTGGGAGGGCCTGAGTATGTGCACTGTCTGCTGCCACCCCTTG
  AGTCACTGGCCACAGTGGAAGAGACAGTAGTGCGAGACAAGGCGGTAGAATCCTTGCGGGCCATCTCTCA
  TGAGCACTCACCTTCCGATCTAGAGGCTCACTTTGTGCCTCTGGTAAAGCGGCTGGCGGGTGGAGACTGG
  TTCACCTCCCGCACCTCGGCCTGTGGTCTCTTCTCAGTTTGCTACCCCCGAGTATCCAGTGCCGTGAAGG
  CAGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATGGTGCGGCGGGCCGCTGCCTC
  CAAGCTGGGGGAATTTGCCAAGGTACTGGAGCTGGACAATGTCAAGAGTGAGATCATTCCCATGTTCTCT
  AACCTGGCCTCTGACGAGCAGGACTCGGTGCGGCTGCTGGCAGTGGAGGCATGTGTGAATATTGCCCAGC
  TTCTGCCACAGGAGGACCTGGAGGCCTTAGTGATGCCCACCTTGCGACAGGCTGCTGAGGACAAGTCTTG
  GCGTGTTCGCTACATGGTGGCCGACAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAG
  ACAGATCTGGTGCCTGCCTTCCAGAACCTGATGAAGGACTGTGAGGCCGAGGTGAGGGCCGCAGCCTCCC
  ACAAGGTCAAAGAGTTCTGTGAAAATCTCTCAGCTGACTGCCGGGAGAATGTGATCATGACTCAGATCTT
  GCCCTGCATCAAGGAGCTTGTGTCAGATGCCAACCAACATGTCAAGTCAGCACTGGCTTCAGTCATCATG
  GGCCTCTCTCCCATTCTGGGCAAAGACAACACCATCGAACACCTCTTGCCCCTGTTCTTGGCTCAGCTGA
  AGGATGAGTGTCCTGAAGTCCGACTGAATATCATCTCCAACCTGGATTGTGTGAACGAGGTGATTGGCAT
  CAGGCAGCTCTCTCAGTCCCTGCTTCCTGCCATCGTGGAACTAGCTGAAGATGCCAAGTGGCGAGTGCGG
  CTGGCCATCATTGAATACATGCCTCTGCTGGCTGGACAGCTTGGTGTGGAATTTTTTGATGAGAAACTAA
  ACTCTTTGTGTATGGCCTGGCTAGTGGATCATGTCTATGCTATCCGTGAGGCTGCCACCAGCAACCTTAA
  GAAATTAGTAGAGAAGTTCGGGAAGGAGTGGGCCCATGCCACTATCATCCCCAAGGTCTTAGCCATGTCT
  GGAGACCCTAACTACCTGCACCGAATGACTACACTCTTCTGCATCAATGTGTTGTCTGAGGTCTGTGGAC
  AGGATATCACCACCAAGCACATGCTGCCCACAGTTCTTCGTATGGCAGGGGACCCAGTTGCCAATGTCCG
  CTTCAATGTGGCCAAGTCACTCCAGAAGATAGGACCCATTCTTGATAACAGCACCCTGCAGAGTGAAGTC
  AAGCCCATCCTGGAGAAGCTGACCCAGGACCAGGATGTGGATGTCAAGTACTTTGCCCAGGAGGCTCTGA
  CTGTTCTCTCTCTTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016891
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016891.3, NP_058587.1
• RefSeq Size: 2256 bp
• RefSeq ORF: 1770 bp
• Locus ID: 51792
• UniProt ID: Q76MZ3
• Cytogenetics: 17 A3.2
• Gene Summary: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit (PubMed:10100624). Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (By similarity). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (By similarity).[UniProtKB/Swiss-Prot Function]