Trp73 (NM_001126330) Mouse Untagged Clone

CAT#: MC219403

Trp73 (untagged) - Mouse transformation related protein 73 (Trp73), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trp73
• Synonyms: delta; deltaNp73; p7; p73; TAp; TAp73; Tp73
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219403 representing NM_001126330
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTACGTCGGTGACCCCATGAGACACCTCGCCACGGCCCAGTTCAATTTGCTCAGCAGTGCCATGG
  ACCAGATGGGCAGCCGTGCGGCCCCGGCGAGCCCCTACACCCCGGAGCACGCCGCCAGCGCGCCCACCCA
  CTCGCCCTACGCGCAGCCCAGCTCCACCTTCGACACCATGTCTCCGGCGCCTGTCATCCCTTCCAATACC
  GACTACCCCGGCCCCCACCACTTCGAGGTCACCTTCCAGCAGTCGAGCACTGCCAAGTCGGCCACCTGGA
  CATACTCCCCACTCTTGAAGAAGTTGTACTGTCAGATTGCTAAGACATGCCCCATCCAGATCAAAGTGTC
  CACACCACCACCCCCGGGCACGGCCATCCGGGCCATGCCTGTCTACAAGAAGGCAGAGCATGTGACCGAC
  ATTGTTAAGCGCTGCCCCAACCACGAGCTTGGAAGGGACTTCAATGAAGGACAGTCTGCCCCGGCTAGCC
  ACCTCATCCGTGTAGAAGGCAACAACCTCGCCCAGTACGTGGATGACCCTGTCACCGGAAGGCAGAGTGT
  GGTTGTGCCGTATGAACCCCCACAGGTGGGAACAGAATTTACCACCATCCTGTACAACTTCATGTGTAAC
  AGCAGCTGTGTGGGGGGCATGAATCGGAGGCCCATCCTTGTCATCATCACCCTGGAGACCCGGGATGGAC
  AGGTCCTGGGCCGCCGGTCTTTCGAGGGTCGCATCTGTGCCTGTCCTGGCCGTGACCGCAAAGCTGATGA
  AGACCATTACCGGGAGCAACAGGCTCTGAATGAAAGTACCACCAAAAATGGAGCTGCCAGCAAACGTGCA
  TTCAAGCAGAGCCCCCCTGCCATCCCTGCCCTGGGTACCAACGTGAAGAAGAGACGCCACGGGGACGAGG
  ACATGTTCTACATGCACGTGCGAGGCCGGGAGAACTTTGAGATCTTGATGAAAGTCAAGGAGAGCCTAGA
  ACTGATGGAGCTTGTGCCCCAGCCTTTGGTTGACTCCTATCGACAGCAGCAGCAGCAGCAGCTCCTACAG
  AGGCCGAGTCACCTGCAGCCTCCATCCTATGGGCCCGTGCTCTCCCCAATGAACAAGGTACACGGTGGTG
  TCAACAAACTGCCCTCCGTCAACCAGCTGGTGGGCCAGCCTCCCCCGCACAGCTCAGCAGCTGGGCCCAA
  CCTGGGGCCCATGGGCTCCGGGATGCTCAACAGCCACGGCCACAGCATGCCGGCCAATGGTGAGATGAAT
  GGAGGCCACAGCTCCCAGACCATGGTTTCGGGATCCCACTGCACCCCGCCACCCCCCTATCATGCAGACC
  CCAGCCTCGTCAGTTTTTTGACAGGGTTGGGGTGTCCAAACTGCATCGAGTGCTTCACTTCCCAAGGGTT
  GCAGAGCATCTACCACCTGCAGAACCTTACCATCGAGGACCTTGGGGCTCTGAAGGTCCCTGACCAGTAC
  CGTATGACCATCTGGAGGGGCCTACAGGACCTGAAGCAGAGCCATGACTGCGGCCAGCAACTGCTACGCT
  CCAGCAGCAACGCGGCCACCATCTCCATCGGCGGCTCTGGCGAGCTGCAGCGGCAGCGGGTCATGGAAGC
  CGTGCATTTCCGTGTGCGCCACACCATCACGATCCCCAACCGTGGAGGCGCAGGTGCGGTGACAGGTCCC
  GACGAGTGGGCGGACTTTGGCTTTGACCTGCCTGACTGCAAGTCCCGTAAGCAGCCCATCAAAGAGGAGT
  TCACAGAGACAGAGAGCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001126330
• Insert Size: 1773 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001126330.1, NP_001119802.1
• RefSeq Size: 4756 bp
• RefSeq ORF: 1773 bp
• Locus ID: 22062
• UniProt ID: Q9JJP2
• Cytogenetics: 4 83.79 cM
• Gene Summary: This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) is an alternate promoter product; it lacks a few of 5' exons but has an alternate 5' exon, as compared to variant 1. The resulting isoform b, also known as DNp73alpha, has a shorter and different N-terminus, as compared to isoform a.