Npas1 (NM_008718) Mouse Untagged Clone

CAT#: MC219392

Npas1 (untagged) - Mouse neuronal PAS domain protein 1 (Npas1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Npas1
• Synonyms: bHLHe11; MOP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219392 representing NM_008718
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCCCCTATCCCAGAAGCGGTGGCCGAGGTGAAGTCAAGTGCGGGGGCGGCCGTGGAGCCGGCG
  TCCCCTGGGATTTCCTGCCTGGGCTGATGGTCAAGGCCCCGCCCGGACCCTGCCTGCAGGCGCAGCGCAA
  AGAGAAGTCCCGCAACGCTGCGCGCTGGCGACGCGGGAAGGAAAATCTGGAGTTCTTCGAGCTAGCCAAG
  CTGCTCCCTCTGCCCGGTGCCATCTCCAGCCAGCTGGACAAGGCGTCCATCGTGCGTCTTAGCGTCACCT
  ACCTCCGCCTGCGTCGTTTTGCCGCGCTGGGGGCGCCGCCCTGGGGGTTGCGGGCCGTCGGGCCTCCGGC
  TGGCCTCGCCCCAGGCCGCCGAGGCCCTGTGGCTCTGGTCTCTGAAGTCTTCGAGCAACACCTAGGTGGA
  CACATCCTACAGTCCTTGGATGGCTTCGTGTTCGCTTTGAACCAGGAAGGGAAATTTCTCTACATCTCAG
  AGACAGTGTCCATCTACCTGGGTCTCTCACAGGTGGAGCTGACGGGCAGCAGCGTCTTCGACTACATCCA
  TCCTGGGGACCACTCGGAGGTCCTAGAGCAACTTGGATTGCGGGCTGCAAGCATCGGTCCCCCTACACCA
  CCTTCCGTGTCCTCCTCTTCCTCATCGTCTTCCTCCTCGCTGGTGGACACCCCTGAGATTGAAGCCAGCC
  CCACCGAGGCATCGCCTGCCTTCCGAGCCCAAGAGCGGTCCTTCTTTGTCCGCATGAAGTCCACCCTCAC
  CAAGAGGGGCCTGAATGTCAAAGCCTCGGGGTACAAGGTCATTCATGTGACAGGGCGCCTGAGGGCCCGA
  GCCCTGGGTCTTGTAGCCCTTGGACACACGCTGCCCCCAGCCCCACTGGCTGAGCTGCCTTTGCACGGAC
  ACATGATTGTCTTCCGCCTCAGCCTGGGCCTCACCATCCTTGCTTGTGAGAGCAGAGTTAGCGACCATAT
  GGACATGGGGCCCTCAGAGCTTGTGGGACGCAGCTGCTACCAGTTTGTTCATGGACAGGATGCAACCAGG
  ATCCGCCAAAGCCATCTGGACCTGCTGGACAAAGGGCAGGTGGTGACTGGTTACTACCGTTGGCTGCAGC
  GTGCGGGGGGCTTCGTGTGGCTGCAGTCTGTAGCCACTGTGGCCGGGAACGGGAAGAGCACTGGGGAGCA
  TCACGTGCTGTGGGTCAGTCACGTGCTCAGCAATGCTGAAGGTAGTCAAACACCCCTGGATGCCTTCCAG
  CTTCCAGCTATTGTGTCTCAGGAGGAGCCATCCAGGCCAGGCCCAGAGCCCACAGAGGAAGAGCCTCCAG
  TTGACGGGAAGCAGGCTGTGCCTGCGGACCAGGACAAGGACAAGGACCCTCAGGCCCGAGGCAAACGCAT
  CAAAGTGGAGGCCAGCCCGAAGGAAGCTAGAGGCTCAGAGGACAGTGGAGAAGAGGAGCTCTCGGATCCA
  CCGGCTCCACCTCGGCCAGAATTCACTTCTGTCATCCGGGCGGGAGCCCTGAAGCATGATCCAGTGCTGC
  CGTGGGGCTTGACAACTCCCGGAGACCCCTCACCCGCCCTCCTTCATGCAGGCTTCCTGCCACCCGTTGT
  GCGGGGCCTGTGCACCCCAGGCACCATCCGCTATGGCCCTGCGGAGTTGAGCCTGATGTATCCACATCTG
  CACAGGCTGGGCGCAGGCCCCTCGCTTCCAGAGGCCTTTTACCCTACGCTGGGCTTGCCCTATCCGGGGC
  CCACAGGTACTAGGGTGCAGCGGAAGGGAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008718
• Insert Size: 1785 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008718.2, NP_032744.1
• RefSeq Size: 2091 bp
• RefSeq ORF: 1785 bp
• Locus ID: 18142
• UniProt ID: P97459
• Cytogenetics: 7 9.14 cM
• Gene Summary: May control regulatory pathways relevant to schizophrenia and to psychotic illness. May play a role in late central nervous system development by modulating EPO expression in response to cellular oxygen level. Forms a heterodimer that binds core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) leading to transcriptional repression on its target gene TH (PubMed:27782878).[UniProtKB/Swiss-Prot Function]