Myef2 (NM_001162417) Mouse Untagged Clone

CAT#: MC219391

Myef2 (untagged) - Mouse myelin basic protein expression factor 2, repressor (Myef2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myef2
• Synonyms: 9430071B01; mKIAA1341
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219391 representing NM_001162417
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGATGCCGACAAGTCCGAGGCGGCCGCGGGTGACGACGGCTCGCAGCAGCAACCGGCTGAGCCGC
  GGCGAGACACGCACCCCGGCGAGCCCGAGAAACCGCCGCGCAGCAGCGCCAACGGCGTTAAAATGGAGAA
  TGATGAGTCAGTGAAGGAAGAAAAATCTGATTTAAAAGAAAAGTCTACAGGAAATAAGAAGGCCAATCGG
  TTCCATCCTTATTCAAAAGATAAGAATTCGGGCACTGGAGAAAAGAAAGGTCCAAATCGGAACAGAGTGT
  TCATTAGCAACATACCCTATGACATGAAGTGGCAGGCTATTAAAGATTTGATGAGAGAAAAAGTTGGTGA
  GGTTACATACGTGGAGCTCTTTAAGGATGCGGAAGGAAAATCAAGGGGTTGTGGCGTGGTTGAATTCAAA
  GATGAAGAATTTGTAAAGAAAGCATTAGAAACTATGAACAAATATGACCTTAGTGGAAGGCCTTTGAATA
  TTAAAGAGGATCCGGATGGAGAAAATGCTCGCCGGGCACTGCAGCGAACAGGAACATCATTTCAAGGATC
  ACATGCCTCCGATGTGGGATCTGGGTTGGTGAATTTACCACCTTCCATTCTCAATAATCCAAACATTCCT
  CCTGAGGTTATCAGCAATTTGCAGGCTGGTAGACTTGGTTCCACAATTTTTGTTGCTAATCTTGACTTCA
  AAGTTGGTTGGAAGAAATTAAAGGAAGTGTTCAGCATAGCTGGAACTGTGAAGCGAGCTGATATTAAAGA
  AGACAAGGATGGCAAGAGCAGAGGCATGGGCACTGTCACTTTTGAGCAGGCAATTGAAGCAGTCCAAGCA
  ATTTCCATGTTCAATGGGCAGTTTTTATTTGATAGACCTATGCATGTGAAAATGGATGACAAATCTGTCC
  CTCATGAAGACTACCGTTCACATGATAGTAAGACATCACAGTTACCACGTGGTCTTGGAGGCATTGGAAT
  GGGACTTGGTCCAGGTGGACAGCCTATTAGTGCCAGCCAGCTGAACATAACTGGTGTAATGGGAAATTTG
  GGTCCAAGTGGTATGGGAATGGATGGTCCGGGTTTTGGAGGAGTGAATAGAATTGGAGGAGGAGTTGGGT
  TTGGTGGTCTGGAAGCAATGAATAGCATGGCAGGCTTTGGTGGAGTTGGCCGAATGGGAGAGCTATACCG
  TGGTGCAATGACTAGTAGCATGGAGCGAGATTTCGGACGTGGTGATATTGGATTAAGTCGAGGCTTTGGC
  GATTCCTTTGGTAGACTTGGCAGTGCAATGATTGGAGGGTTTGCAGGAAGAATAGGAGCTTCTAACATGG
  GTCCAGTAGGAACTGGAATAAGCGGCAGCATGAGCGGCATGAGCACTGTGACTGGAGGCATGGGCATGGG
  ACTGGACCGCATGAGCTCCAGCTTCGACAGGATGGGGCCAGGCATTGGAGCCATACTGGAAAGGAGCATC
  GATGTAGACCGAGGGTTTTTATCAGGTCCCATGGGAAGCGGAATGAGAGACAGATTAGGCTCCAAAGGCA
  ACCAGATATTTGTTAGAAATCTTCCTTTTGACTTGACTTGGCAGAAATTAAAAGAGAAATTCAGCCAATG
  TGGTCATGTAATGTTTGCAGAGATAAAGATGGAGAATGGCAAGTCAAAAGGCTGTGGGACAGTCAGGTTT
  GAATCTGCAGAGTCAGCGGAAAAGGCCTGCAGGATCATGAATGGCATCAAGATCAGCGGCAGGGAAATCG
  ATGTGCGCTTGGACCGCAATGCGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001162417
• Insert Size: 1776 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162417.1, NP_001155889.1
• RefSeq Size: 2402 bp
• RefSeq ORF: 1776 bp
• Locus ID: 17876
• UniProt ID: Q8C854
• Cytogenetics: 2 61.16 cM
• Gene Summary: Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).