Cry2 (NM_001113333) Mouse Untagged Clone

CAT#: MC219379

Cry2 (untagged) - Mouse cryptochrome 2 (photolyase-like) (Cry2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cry2
• Synonyms: AV006279; D130054K12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219379 representing NM_001113333
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCTGCTGTGGTGGCAGCGACGGTCCCCGCGCAATCGATGGGCGCGGACGGCGCGTCCTCGG
  TGCACTGGTTCCGCAAAGGACTACGGCTCCACGACAACCCCGCGCTGCTAGCTGCCGTGCGCGGGGCGCG
  CTGTGTGCGCTGCGTCTACATCCTCGACCCGTGGTTCGCGGCCTCCTCGTCTGTGGGCATCAACCGATGG
  AGGTTCCTACTGCAATCTCTGGAAGATCTGGACACAAGCTTAAGAAAGCTGAATTCGCGTCTGTTTGTAG
  TCCGGGGACAGCCAGCTGATGTGTTCCCAAGGCTGTTCAAGGAATGGGGGGTGACCCGTTTGACCTTTGA
  ATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATTATGAAGATGGCCAAGGAGGCTGGCGTG
  GAGGTGGTGACTGAGAACTCTCACACCCTCTATGACCTAGACAGAATCATCGAACTGAATGGGCAGAAAC
  CACCCCTTACCTACAAGCGCTTTCAGGCCCTCATCAGCCGCATGGAGCTGCCCAAGAAGCCCGCGGTGGC
  TGTGAGCAGCCAGCAGATGGAGAGCTGCAGAGCTGAGATCCAGGAGAACCATGACGACACCTATGGCGTG
  CCTTCCCTGGAGGAGCTGGGATTCCCCACGGAAGGACTTGGCCCAGCTGTTTGGCAAGGAGGAGAGACAG
  AAGCTCTGGCCCGCCTGGACAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCTCGGAT
  GAATGCCAATTCCTTACTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGATGCCTCTCCTGC
  CGCCTCTTCTACTACCGCCTGTGGGACTTGTACAAGAAGGTGAAGAGGAACAGCACACCCCCCCTCTCCT
  TATTTGGACAACTCCTGTGGCGAGAATTCTTCTACACAGCGGCCACCAACAACCCCAGGTTTGACCGAAT
  GGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAACCCCGAAGCCCTGGCCAAGTGGGCCGAG
  GGCAAGACAGGCTTCCCTTGGATTGACGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACC
  TGGCCCGGCACGCTGTGGCCTGCTTCCTCACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCG
  GGTATTTGACGAGCTGCTCCTGGATGCCGATTTCAGTGTGAATGCAGGCAGCTGGATGTGGCTGTCCTGC
  AGTGCTTTCTTCCAACAATTCTTCCACTGCTACTGCCCTGTGGGCTTCGGCCGACGTACAGACCCCAGTG
  GGGACTACATCCGGCGGTACCTGCCCAAACTGAAAGGCTTCCCCTCTCGATACATCTATGAGCCCTGGAA
  TGCCCCCGAGTCAGTTCAGAAGGCTGCCAAGTGCATCATTGGCGTGGACTACCCACGGCCCATCGTCAAT
  CATGCAGAGACTAGTCGGCTCAACATTGAACGAATGAAGCAGATCTACCAACAGCTGTCGAGATACCGGG
  GACTCTGTCTATTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCTGGTTC
  AAGCCAAGCTGGGAGCATCAGCAACACAGGCCCCAGAGCACTATCCAGTGGCCCAGCTTCCCCCAAACGC
  AAGCTGGAAGCAGCCGAGGAACCTCCTGGTGAAGAACTGACCAAGCGGGCTAGAGTGACGGAGATGCCTA
  CCCAAGAGCCAGCAAGCAAGGACTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113333
• Insert Size: 1779 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113333.1, NP_001106804.1
• RefSeq Size: 2193 bp
• RefSeq ORF: 1779 bp
• Locus ID: 12953
• Cytogenetics: 2 E1
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]