Alas2 (NM_009653) Mouse Untagged Clone

CAT#: MC219374

Alas2 (untagged) - Mouse aminolevulinic acid synthase 2, erythroid (Alas2), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alas2
• Synonyms: ALAS; Alas-2; ALAS-E; ALASE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219374 representing NM_009653
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCAGCAGCTATGTTGCTACGGTCCTGTCCAGTGCTCTCTCAGGGCCCCACAGGCCTCCTGGGCA
  AAGTGGCTAAAACCTACCAGTTCCTATTTAGTATTGGACGCTGCCCCATCCTGGCCACTCAAGGACCAAC
  CTGTTCTCAAATCCATCTTAAGGCAACCAAGGCTGGAGGAGACTCTCCATCTTGGGCTAAGAGCCATTGT
  CCTTTCATGCTGTCAGAACTCCAAGACAGGAAGAGCAAGATTGTGCAGAGGGCAGCTCCAGAAGTTCAAG
  AGGATGTCAAGACTTTCAAGACAGACCTGCTGAGCACCATGGATTCAACCACCCGAAGCCATTCATTTCC
  TAGTTTCCAGGAGCCAGAGCAGACTGAAGGGGCAGTTCCCCACCTGATTCAGAACAATATGACTGGAAGC
  CAGGCTTTCGGTTATGACCAATTTTTCAGAGACAAGATCATGGAGAAGAAACAGGACCACACCTACCGTG
  TGTTCAAGACTGTGAATCGTTGGGCTAATGCCTACCCCTTTGCCCAACACTTCTCCGAGGCATCTATGGC
  ATCAAAGGATGTTTCTGTTTGGTGTAGTAATGACTATTTGGGCATAAGCAGACACCCTCGTGTCTTGCAG
  GCCATAGAGGAGACCCTGAAGAATCATGGAGCTGGAGCTGGGGGCACTCGCAATATCTCAGGTACCAGCA
  AGTTTCATGTGGAGCTTGAACAGGAGCTGGCTGAACTACACCAGAAAGACTCAGCTCTGCTCTTCTCCTC
  CTGTTTTGTGGCCAATGATTCTACTCTCTTTACACTGGCCAAGCTTCTGCCAGGGTGTGAGATCTACTCA
  GATGCAGGCAATCATGCCTCCATGATCCAAGGCATTCGCAACAGTGGTGCAGCCAAGTTTGTCTTCAGAC
  ACAATGACCCAGGCCACCTGAAGAAACTTCTCGAGAAGTCTGATCCCAAGACACCAAAAATTGTGGCTTT
  TGAGACTGTTCATTCCATGGATGGTGCCATCTGTCCTCTGGAGGAATTGTGTGATGTGGCCCACCAGTAT
  GGAGCCCTGACCTTCGTAGATGAAGTCCATGCTGTAGGACTGTATGGAGCCCGGGGTGCAGGTATCGGGG
  AGCGTGATGGAATTATGCACAAGCTTGACATCATCTCTGGAACTCTTGGCAAGGCCTTTGGTTGCGTCGG
  TGGCTATATAGCCAGCACTCGGGACTTGGTGGACATGGTGCGCTCCTACGCTGCAGGCTTCATCTTTACC
  ACTTCACTGCCTCCCATGGTGCTCTCTGGGGCTCTAGAATCTGTGCGCCTACTCAAGGGAGAGGAGGGTC
  AAGCCCTGAGGCGGGCACACCAGCGCAATGTCAAACACATGCGCCAGCTGCTAATGGACAGGGGCTTTCC
  TGTTATCCCCTGTCCCAGCCACATCATCCCCATCAGGGTGGGTAATGCAGCACTCAACAGCAAGATCTGT
  GATCTTCTGCTCTCCAAGCACAGCATCTATGTGCAGGCCATCAACTACCCAACTGTGCCTCGTGGTGAGG
  AGCTACTGCGCTTGGCCCCCTCCCCCCACCACAGCCCTCAGATGATGGAAAACTTTGTGGAGAAGCTGCT
  GCTGGCCTGGACTGAGGTGGGGCTGCCCCTCCAAGATGTGTCTGTGGCTGCATGCAACTTCTGTCATCGT
  CCTGTGCACTTTGAACTTATGAGCGAGTGGGAGCGATCCTACTTTGGGAACATGGGACCCCAATATGTTA
  CCACCTATGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009653
• Insert Size: 1764 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009653.3, NP_033783.1
• RefSeq Size: 1983 bp
• RefSeq ORF: 1764 bp
• Locus ID: 11656
• UniProt ID: P08680
• Cytogenetics: X 68.46 cM